Roombia Truncata
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Roombia Truncata
''Roombia truncata'' is a species of katablepharids, which are heterotrophic single-celled organisms. It was the first katablepharid to be generally available in cell culture, starting around 2009. The culture consists of ''Roombia'', the diatom ''Navicula ''Navicula'' is a genus of boat-shaped diatom (single-celled photosynthetic organisms), comprising over 1,200 species, though many ''Navicula'' species likely do not belong in the genus strictly speaking. ''Navicula'' is Latin for "small ship", ...'', and unidentified bacteria. ''Navicula'' provides the main food source for ''Roombia'', although ''Roombia'' also feeds on the bacteria. The Roombia Truncata was identified through SSU rRNA gene analysis as a typical component of eukaryotes however its complete set of RNA transcripts show mgR2-like CCr (Light sensitive ion channel) unlike other cryptists within this branch of the eukaryotic tree of life. ''Roombia'' was named after the Roomba robotic vacuum cleaner. Re ...
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Katablepharid
The kathablepharids or katablepharids () are a group of heterotrophic flagellates closely related to cryptomonads. First described by Heinrich Leonhards Skuja in 1939, kathablepharids were named after the genus '' Kathablepharis''. This genus is corrected to ''Katablepharis'' under botanical nomenclature, but the original spelling is maintained under zoological nomenclature. They are single-celled protists with two anteriorly directed flagella, an anterior cytostome for ingesting eukaryotic prey, and a sheath that covers the cell membrane. They have extrusomes known as ejectisomes, as well as tubular mitochondrial cristae. Evolution Besides the known katablepharid diversity, dozens of environmental DNA sequences (both freshwater and marine) seem to represent further katablepharids which have not been cultured or formally described. Through molecular phylogenetic analyses, they are consistently recovered as the sister clade to cryptomonads, an assemblage of flagellates containin ...
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Light Microscopy
Microscopy is the technical field of using microscopes to view subjects too small to be seen with the naked eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy, along with the emerging field of X-ray microscopy. Optical microscopy and electron microscopy involve the diffraction, reflection, or refraction of electromagnetic radiation/electron beams interacting with the specimen, and the collection of the scattered radiation or another signal in order to create an image. This process may be carried out by wide-field irradiation of the sample (for example standard light microscopy and transmission electron microscopy) or by scanning a fine beam over the sample (for example confocal laser scanning microscopy and scanning electron microscopy). Scanning probe microscopy involves the interaction of a scanning probe with the surface of the object of interest. The de ...
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Transmission Electron Micrograph
Transmission electron microscopy (TEM) is a microscopy technique in which a beam of electrons is transmitted through a specimen to form an image. The specimen is most often an ultrathin section less than 100 nm thick or a suspension on a grid. An image is formed from the interaction of the electrons with the sample as the beam is transmitted through the specimen. The image is then magnified and Focus (optics), focused onto an imaging device, such as a fluorescent screen, a layer of photographic film, or a Detectors for transmission electron microscopy, detector such as a scintillator attached to a charge-coupled device or a direct electron detector. Transmission electron microscopes are capable of imaging at a significantly higher Optical resolution, resolution than Optical microscope, light microscopes, owing to the smaller de Broglie wavelength of electrons. This enables the instrument to capture fine detail—even as small as a single column of atoms, which is thousands o ...
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Heterotrophic
A heterotroph (; ) is an organism that cannot produce its own food, instead taking nutrition from other sources of organic carbon, mainly plant or animal matter. In the food chain, heterotrophs are primary, secondary and tertiary consumers, but not producers. Living organisms that are heterotrophic include all animals and fungi, some bacteria and protists, and many parasitic plants. The term heterotroph arose in microbiology in 1946 as part of a classification of microorganisms based on their type of nutrition. The term is now used in many fields, such as ecology, in describing the food chain. Heterotrophs occupy the second and third trophic levels of the food chain while autotrophs occupy the first trophic level. Heterotrophs may be subdivided according to their energy source. If the heterotroph uses chemical energy, it is a chemoheterotroph (e.g., humans and mushrooms). If it uses light for energy, then it is a photoheterotroph (e.g., green non-sulfur bacteria). Heterotrop ...
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Cell Culture
Cell culture or tissue culture is the process by which cell (biology), cells are grown under controlled conditions, generally outside of their natural environment. After cells of interest have been Cell isolation, isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. They need to be kept at body temperature (37 °C) in an incubator. These conditions vary for each cell type, but generally consist of a suitable vessel with a substrate or rich growth medium, medium that supplies the essential nutrients (amino acids, carbohydrates, vitamins, minerals), growth factors, hormones, and gases (Carbon dioxide, CO2, Oxygen, O2), and regulates the physio-chemical environment (Buffer solution, pH buffer, osmotic pressure, temperature). Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture. T ...
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Navicula
''Navicula'' is a genus of boat-shaped diatom (single-celled photosynthetic organisms), comprising over 1,200 species, though many ''Navicula'' species likely do not belong in the genus strictly speaking. ''Navicula'' is Latin for "small ship", and also a term in English for a boat-shaped incense-holder.Oxford English Dictionary, "Navicula. 3" ''Navicula'' is a Cosmopolitan distribution, cosmopolitan genus and species are present in both freshwater and marine environments, typically attached to surfaces (i.e. Benthic zone, benthic). Description ''Navicula'' species are pennate diatoms. Their valves are typically elliptical, though some species have more pinched ends than others. ''Navicula'' cells have two Chloroplast, chloroplasts, one along each side of the valve along the girdle bands. Mobility ''Navicula'' diatoms are highly motile and move through a gliding movement This is done through excretion of Extracellular polymeric substance, extracellular polymeric substances (E ...
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Roomba
A Roomba is an autonomous robotic vacuum cleaner made by the company iRobot, and was first introduced in September 2002. Roombas have a set of sensors which help them navigate the floor area of a home. These sensors can detect the presence of obstacles and steep drops (e.g., to avoid falling down stairs). As of 2024, iRobot markets models of their fourth through tenth generation, while continuing to provide support and to sell accessories for their previous series. Various models of the Roomba have different features, including tangle-free brushes, separate sweep canisters, more powerful vacuums, obstacle avoidance, and performance maps displayed via smartphone apps. Newer models also have a camera, which works in conjunction with onboard mapping and navigation software to systematically cover all floor areas, move from room to room, avoid obstacles such as pet waste and charging cables, and find charging stations. History Engineer Joe Jones first conceived the idea fo ...
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