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Forensic DNA Analysis
DNA profiling is the determination of a DNA profile for legal and investigative purposes. DNA analysis methods have changed numerous times over the years as technology improves and allows for more information to be determined with less starting material. Modern DNA analysis is based on the statistical calculation of the rarity of the produced profile within a population. While most well known as a tool in forensic investigations, DNA profiling can also be used for non-forensic purposes such as paternity testing and human genealogy research. History The methods for producing a DNA profile were developed by Alec Jeffreys and his team in 1984. Methods Retired methods RFLP analysis The first true method of DNA profiling was restriction fragment length polymorphism analysis. The first use of RFLP analysis in forensic casework was in 1985 in the United Kingdom. This type of analysis used variable number tandem repeats (VNTRs) to distinguish between individuals. VNT ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
DNA Profile
DNA profiling (also called DNA fingerprinting) is the process of determining an individual's DNA characteristics. DNA analysis intended to identify a species, rather than an individual, is called DNA barcoding. DNA profiling is a forensic technique in criminal investigations, comparing criminal suspects' profiles to DNA evidence so as to assess the likelihood of their involvement in the crime. It is also used in paternity testing, to establish immigration eligibility, and in genealogical and medical research. DNA profiling has also been used in the study of animal and plant populations in the fields of zoology, botany, and agriculture. Background Starting in the 1980s, scientific advances allowed the use of DNA as a material for the identification of an individual. The first patent covering the direct use of DNA variation for forensicsUS5593832A was filed by Jeffrey Glassberg in 1983, based upon work he had done while at Rockefeller University in the United States in 1981. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Likelihood Function
The likelihood function (often simply called the likelihood) represents the probability of random variable realizations conditional on particular values of the statistical parameters. Thus, when evaluated on a given sample, the likelihood function indicates which parameter values are more ''likely'' than others, in the sense that they would have made the observed data more probable. Consequently, the likelihood is often written as \mathcal(\theta\mid X) instead of P(X \mid \theta), to emphasize that it is to be understood as a function of the parameters \theta instead of the random variable X. In maximum likelihood estimation, the arg max of the likelihood function serves as a point estimate for \theta, while local curvature (approximated by the likelihood's Hessian matrix) indicates the estimate's precision. Meanwhile in Bayesian statistics, parameter estimates are derived from the converse of the likelihood, the so-called posterior probability, which is calculated via Baye ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Prosecutor's Fallacy
The prosecutor's fallacy is a fallacy of statistical reasoning involving a test for an occurrence, such as a DNA match. A positive result in the test may paradoxically be more likely to be an erroneous result than an actual occurrence, even if the test is very accurate. The fallacy is named because it is typically used by a prosecutor to exaggerate the probability of a criminal defendant's guilt. The fallacy can be used to support other claims as well – including the innocence of a defendant. For instance, if a perpetrator were known to have the same blood type as a given defendant and 10% of the population to share that blood type, then one version of the prosecutor's fallacy would be to claim that, on that basis alone, the probability that the defendant is guilty is 90%. However, this conclusion is only close to correct if the defendant was selected as the main suspect based on robust evidence discovered prior to the blood test and unrelated to it (the blood match may t ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Rapid DNA Act Of 2017
Rapids are sections of a river where the river bed has a relatively steep gradient, causing an increase in water velocity and turbulence. Rapids are hydrological features between a ''run'' (a smoothly flowing part of a stream) and a '' cascade''. Rapids are characterized by the river becoming shallower with some rocks exposed above the flow surface. As flowing water splashes over and around the rocks, air bubbles become mixed in with it and portions of the surface acquire a white color, forming what is called "whitewater". Rapids occur where the bed material is highly resistant to the erosive power of the stream in comparison with the bed downstream of the rapids. Very young streams flowing across solid rock may be rapids for much of their length. Rapids cause water aeration of the stream or river, resulting in better water quality. Rapids are categorized in classes, generally running from I to VI. A Class 5 rapid may be categorized as Class 5.1-5.9. While Class I rapids are ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Mutation
In biology, a mutation is an alteration in the nucleic acid sequence of the genome of an organism, virus, or extrachromosomal DNA. Viral genomes contain either DNA or RNA. Mutations result from errors during DNA replication, DNA or viral replication, mitosis, or meiosis or other types of DNA repair#DNA damage, damage to DNA (such as pyrimidine dimers caused by exposure to ultraviolet radiation), which then may undergo error-prone repair (especially microhomology-mediated end joining), cause an error during other forms of repair, or cause an error during replication (DNA repair#Translesion synthesis, translesion synthesis). Mutations may also result from Insertion (genetics), insertion or Deletion (genetics), deletion of segments of DNA due to mobile genetic elements. Mutations may or may not produce detectable changes in the observable characteristics (phenotype) of an organism. Mutations play a part in both normal and abnormal biological processes including: evolution, cancer ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Familial Searching
DNA profiling (also called DNA fingerprinting) is the process of determining an individual's DNA characteristics. DNA analysis intended to identify a species, rather than an individual, is called DNA barcoding. DNA profiling is a forensic technique in criminal investigations, comparing criminal suspects' profiles to DNA evidence so as to assess the likelihood of their involvement in the crime. It is also used in paternity testing, to establish immigration eligibility, and in genealogical and medical research. DNA profiling has also been used in the study of animal and plant populations in the fields of zoology, botany, and agriculture. Background Starting in the 1980s, scientific advances allowed the use of DNA as a material for the identification of an individual. The first patent covering the direct use of DNA variation for forensicsUS5593832A was filed by Jeffrey Glassberg in 1983, based upon work he had done while at Rockefeller University in the United States in 1981. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Y-STR
A Y-STR is a short tandem repeat (STR) on the Y-chromosome. Y-STRs are often used in forensics, paternity, and genealogical DNA testing. Y-STRs are taken specifically from the male Y chromosome. These Y-STRs provide a weaker analysis than autosomal STRs because the Y chromosome is only found in males, which are only passed down by the father, making the Y chromosome in any paternal line practically identical. This causes a significantly smaller amount of distinction between Y-STR samples. Autosomal STRs provide a much stronger analytical power because of the random matching that occurs between pairs of chromosomes during the zygote making process. Nomenclature Y-STRs are assigned names by the HUGO gene nomenclature committee (HGNC). Some testing companies have different formats for the way STR markers are written. For example, the marker DYS455 may be written as DYS455, DYS 455, DYS#455, or DYS# 455. The scientific standard accepted by HUGO and NIST is DYS455. DYS DYS is a vari ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Y Chromosome
The Y chromosome is one of two sex chromosomes (allosomes) in therian mammals, including humans, and many other animals. The other is the X chromosome. Y is normally the sex-determining chromosome in many species, since it is the presence or absence of Y that determines the male or female sex of offspring produced in sexual reproduction. In mammals, the Y chromosome contains the gene SRY, which triggers male development. The DNA in the human Y chromosome is composed of about 59 million base pairs, making it similar in size to chromosome 19. The Y chromosome is passed only from father to son. With a 30% difference between humans and chimpanzees, the Y chromosome is one of the fastest-evolving parts of the human genome. The human Y chromosome carries an estimated 100–200 genes, with between 45 and 73 of these being protein-coding. All single-copy Y-linked genes are hemizygous (present on only one chromosome) except in cases of aneuploidy such as XYY syndrome or XXYY syndrome. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
