Flow Cytometry
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Flow Cytometry
Flow cytometry (FC) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. The sample is focused to ideally flow one cell at a time through a laser beam, where the light scattered is characteristic to the cells and their components. Cells are often labeled with fluorescent markers so light is absorbed and then emitted in a band of wavelengths. Tens of thousands of cells can be quickly examined and the data gathered are processed by a computer. Flow cytometry is routinely used in basic research, clinical practice, and clinical trials. Uses for flow cytometry include: * Cell counting * Cell sorting * Determining cell characteristics and function * Detecting microorganisms * Biomarker detection * Protein engineering detection * Diagnosis of health disorders such as blood cancers * Measur ...
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Cytometry
Cytometry is the measurement of number and characteristics of cells. Variables that can be measured by cytometric methods include cell size, cell count, cell morphology (shape and structure), cell cycle phase, DNA content, and the existence or absence of specific proteins on the cell surface or in the cytoplasm. Cytometry is used to characterize and count blood cells in common blood tests such as the complete blood count. In a similar fashion, cytometry is also used in cell biology research and in medical diagnostics to characterize cells in a wide range of applications associated with diseases such as cancer and AIDS. Cytometric devices Image cytometers Image cytometry is the oldest form of cytometry. Image cytometers operate by statically imaging a large number of cells using optical microscopy. Prior to analysis, cells are commonly stained to enhance contrast or to detect specific molecules by labeling these with fluorochromes. Traditionally, cells are viewed within a hemocyt ...
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Göttingen
Göttingen (, , ; nds, Chöttingen) is a college town, university city in Lower Saxony, central Germany, the Capital (political), capital of Göttingen (district), the eponymous district. The River Leine runs through it. At the end of 2019, the population was 118,911. General information The origins of Göttingen lay in a village called ''Gutingi, ''first mentioned in a document in 953 AD. The city was founded northwest of this village, between 1150 and 1200 AD, and adopted its name. In Middle Ages, medieval times the city was a member of the Hanseatic League and hence a wealthy town. Today, Göttingen is famous for its old university (''Georgia Augusta'', or University of Göttingen, "Georg-August-Universität"), which was founded in 1734 (first classes in 1737) and became the most visited university of Europe. In 1837, seven professors protested against the absolute sovereignty of the House of Hanover, kings of Kingdom of Hanover, Hanover; they lost their positions, but be ...
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