The Trolox equivalent antioxidant capacity (TEAC) assay measures the
antioxidant capacity of a given substance, as compared to the standard,
Trolox
Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) is a water-soluble analog of vitamin E sold by Hoffman-LaRoche. It is an antioxidant like vitamin E and it is used in biological or biochemical applications to reduce oxidative stre ...
. Most commonly, antioxidant capacity is measured using the
ABTS
In biochemistry, ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) is a chemical compound used to observe the reaction kinetics of specific enzymes. A common use for it is in the enzyme-linked immunosorbent assay (ELISA) to detect the ...
Decolorization Assay. Other antioxidant capacity assays which use Trolox as a standard include the diphenylpicrylhydrazyl (
DPPH
DPPH is a common abbreviation for the organic chemical compound 2,2-diphenyl-1-picrylhydrazyl. It is a dark-colored crystalline powder composed of stable free radical molecules. DPPH has two major applications, both in laboratory research: one is ...
), oxygen radical absorbance capacity (
ORAC ORAC or Orac may refer to:
* Oxygen radical absorbance capacity, a scalar value derived in the laboratory for comparing the antioxidant content of different foods or nutritional supplements
* Office of the Registrar of Aboriginal Corporations, for ...
) and ferric reducing ability of plasma (
FRAP) assays. The TEAC assay is often used to measure the antioxidant capacity of foods, beverages and nutritional supplements.
References
Biochemistry detection reactions
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