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Thioglycolate
Thioglycolate
broth is a multipurpose, enriched, differential medium used primarily to determine the oxygen requirements of microorganisms. Sodium thioglycolate in the medium consumes oxygen and permits the growth of obligate anaerobes.[1] This, combined with the diffusion of oxygen from the top of the broth, produces a range of oxygen concentrations in the medium along its depth. The oxygen concentration at a given level is indicated by a redox-sensitive dye such as resazurine that turns pink in the presence of oxygen.

Thioglycolate
Thioglycolate
broth medium is recommended to isolate strict anaerobes should an anaerobic infection be suspected.[2]

This allows the differentiation of obligate aerobes, obligate anaerobes, facultative anaerobes, microaerophiles, and aerotolerant organisms. For example, obligately anaerobic Clostridium
Clostridium
species will be seen growing only in the bottom of the test tube. Thioglycolate
Thioglycolate
broth is also used to recruit macrophages to the peritoneal cavity of mice when injected intraperitoneally.[3] It recruits numerous macrophages, but does not activate them.[3] References[edit]

^ Cain, Donna. "Fluid Thioglycollate [sic] Broth". Collin College.  ^ Cheesbrough, Monica (2006). District Laboratory Practice in Tropical Countries, Part 2. Cambridge University
Cambridge University
Press. p. 126. ISBN 0-521-67631-2. Retrieved 2009-09-14.  ^ a b Leijh PC; van Zwet TL; ter Kuile MN; van Furth R (November 1984). "Effect of thioglycolate on phagocytic and microbicidal activities of peritoneal macrophages". Infection and Immunity. 46 (2): 448–452. PMC 261553 . PMID 6500699. 

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Growth media / agar plates

Selective media

Gram positive

Actinobacteria

Mycobacterium tuberculosis

Löwenstein–Jensen medium Middlebrook 7H9 Broth Middlebrook 7H10 Agar Middlebrook 7H11 Agar

Mycoplasma pneumoniae

Eaton's agar

Firmicutes

Corynebacterium diphtheriae

Hoyle's agar

Enterococcus

Bile esculin agar

Lactobacillus

MRS agar

Lactococcus

M17 agar

Staphylococcus

Mannitol salt agar

Gram negative

Alphaproteobacteria

Brucella

Brucella
Brucella
agar Farrell's medium

Betaproteobacteria

Neisseria

Thayer-Martin agar

Gammaproteobacteria

Bordetella

Bordet-Gengou agar

Enterobacteriaceae

VRBD agar

Haemophilus influenzae/Legionella pneumophila

Buffered charcoal yeast extract agar

Pseudomonas aeruginosa

Cetrimide agar

Salmonella

XLT agar

DCA agar Salmonella/Shigella

XLD agar

Differential media

Lactose
Lactose
fermenting gram negative

MacConkey agar/Sorbitol-MacConkey agar Eosin methylene blue

Hektoen enteric agar sulfur

Bismuth sulfite agar

Fungal media

Czapek medium Dermatophyte test medium Potato dextrose agar Sabouraud agar

Nonselective media

Chocolate agar Nutrient agar Plate count agar

Other/ungrouped media

Cystine lactose electrolyte deficient agar Cystine tryptic agar Endo agar Lysine iron agar slant Müller-Hinton agar/PNP agar R2a agar Simmons' citrate agar Trypticase soy agar Tryptic soy broth TSI slant

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