Second Generation Multiplex

{{technical, date=June 2012 Second Generation Multiplex is a DNA profiling system used in the United Kingdom to set up the

UK National DNA Database The United Kingdom National DNA Database (NDNAD; officially the ''UK National Criminal Intelligence DNA Database'') is a national DNA Database that was set up in 1995. In 2005 it had 3.1 million profiles and in 2020 it had 6.6 million profiles ...

in 1995. It is manufactured by ABI (Applied Biosystems). It contains primers for the following STR (

Short Tandem Repeat A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from one to six or more base pairs) are repeated, typically 5–50 times. Microsatellites occur at thousands of locations within an organism's genome. ...

) loci. VWA (HUMVWF31/A), D8 (D8S1179), D21 (D21S11), D18 (D18S51), THO (HUMTHO1), FGA (HUMFIBRA) Also contains primers for the

Amelogenin Amelogenins are a group of protein isoforms produced by alternative splicing or proteolysis from the ''AMELX'' gene, on the X chromosome, and also the ''AMELY'' gene in males, on the Y chromosome. They are involved in amelogenesis, the developme ...

sex indicating test. The primers are tagged with the following fluorescent dyes for detection under electrophoresis. * 5-FAM *JOE *NED Its use in the United kingdom as the DNA profiling system used by The

was superseded by the

Second Generation Multiplex Plus Second Generation Multiplex Plus (SGM Plus), is a DNA profiling system developed by Applied Biosystems. It is an updated version of Second Generation Multiplex. SGM Plus has been used by the UK National DNA Database since 1998. An SGM Plus profile ...

SGM+ DNA profiling system in 1998 Biometrics DNA profiling techniques Genetics in the United Kingdom Law enforcement in the United Kingdom