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Interferon-gamma release assays (IGRAs) are
diagnostic tool Diagnosis is the identification of the nature and cause of a certain phenomenon. Diagnosis is used in many different disciplines, with variations in the use of logic, analytics, and experience, to determine " cause and effect". In systems engin ...
s for latent tuberculosis infection (LTBI). They are surrogate markers of ''Mycobacterium tuberculosis'' infection and indicate a cellular immune response to ''M. tuberculosis'' if the latter is present.


Active vs latent tuberculosis

IGRAs cannot distinguish between latent infection and active tuberculosis (TB) disease, and should not be used as a sole method for diagnosis of active TB, which is a microbiological diagnosis. A positive IGRA result may not necessarily indicate TB infection, but can also be caused by infection with non-tuberculous mycobacteria. A negative IGRA does not rule out active TB disease; a number of studies have shown that up to a quarter of patients with active TB have negative IGRA results.


BCG Status

Because IGRAs are not affected by
Bacille Calmette-Guérin Bacillus Calmette–Guérin (BCG) vaccine is a vaccine primarily used against tuberculosis (TB). It is named after its inventors Albert Calmette and Camille Guérin. In countries where tuberculosis or leprosy is common, one dose is recommende ...
(BCG) vaccination status, IGRAs are useful for evaluation of LTBI in BCG-vaccinated individuals, particularly in settings where BCG vaccination is administered after infancy or multiple (booster) BCG vaccinations are given. In contrast, the specificity of tuberculin skin test (TST) varies depending on timing of BCG and whether repeated (booster) vaccinations are given.


Commercial IGRA test

QuantiFERON, also known as QFT, is the registered trademark of an
interferon gamma release assay Interferon-γ release assays (IGRA) are medical tests used in the diagnosis of some infectious diseases, especially tuberculosis. Interferon-γ (IFN-γ) release assays rely on the fact that T-lymphocytes will release IFN-γ when exposed to specific ...
(IGRA) for
tuberculosis diagnosis Tuberculosis is diagnosed by finding '' Mycobacterium tuberculosis'' bacteria in a clinical specimen taken from the patient. While other investigations may strongly suggest tuberculosis as the diagnosis, they cannot confirm it. A complete medica ...
manufactured by
QIAGEN QIAGEN N.V., the global corporate headquarter of the QIAGEN group, is located in Venlo, The Netherlands. Furthermore, European, American, and Asia regional headquarters are located in respectively Hilden, Germany, Maryland United States, and Sh ...
. The QFT-GIT assay is an
ELISA The enzyme-linked immunosorbent assay (ELISA) (, ) is a commonly used analytical biochemistry assay, first described by Eva Engvall and Peter Perlmann in 1971. The assay uses a solid-phase type of enzyme immunoassay (EIA) to detect the presence ...
-based, whole-blood test that uses peptides from three TB antigens (ESAT-6, CFP-10, and TB7.7) in an in-tube format. The result is reported as quantification of IFN-gamma in
international unit In pharmacology, the international unit (IU) is a unit of measurement for the effect, not mass of a substance; the variance is based on the biological activity or effect, for the purpose of easier comparison across similar ''forms'' of substan ...
s (IU) per mL. An individual is considered positive for ''M. tuberculosis'' infection if the IFN-gamma response to TB antigens is above the test cut-off (after subtracting the background IFN-gamma response in the negative control).


Mantoux vs IGRA test

Since IGRAs are more costly and technically complex to do than the
Mantoux test The Mantoux test or Mendel–Mantoux test (also known as the Mantoux screening test, tuberculin sensitivity test, Pirquet test, or PPD test for purified protein derivative) is a tool for screening for tuberculosis (TB) and for tuberculosis diagn ...
, in their 2011 policy statement, the
WHO Who or WHO may refer to: * Who (pronoun), an interrogative or relative pronoun * Who?, one of the Five Ws in journalism * World Health Organization Arts and entertainment Fictional characters * Who, a creature in the Dr. Seuss book '' Horton He ...
did not recommend replacing the Mantoux test by IGRAs as a public health intervention in low- and middle-income countries.


QuantiFERON-TB (QFT)

QuantiFERON-TB Gold In-Tube (QFT-GIT), the third generation test, has replaced QuantiFERON-TB (QFT) and QuantiFERON-Gold, which are no longer marketed. According to the
U.S. Centers for Disease Control The Centers for Disease Control and Prevention (CDC) is the National public health institutes, national public health agency of the United States. It is a Federal agencies of the United States, United States federal agency, under the United S ...
, in 2001, the QuantiFERON-TB test (QFT) was approved by the
Food and Drug Administration The United States Food and Drug Administration (FDA or US FDA) is a List of United States federal agencies, federal agency of the United States Department of Health and Human Services, Department of Health and Human Services. The FDA is respon ...
(FDA) as an aid for detecting latent ''
Mycobacterium tuberculosis ''Mycobacterium tuberculosis'' (M. tb) is a species of pathogenic bacteria in the family Mycobacteriaceae and the causative agent of tuberculosis. First discovered in 1882 by Robert Koch, ''M. tuberculosis'' has an unusual, waxy coating on its c ...
'' infection. This test is an in vitro diagnostic aid that measures a component of cell-mediated immune reactivity to ''M. tuberculosis''. The test is based on the quantification of
interferon-gamma Interferon gamma (IFN-γ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. The existence of this interferon, which early in its history was known as immune interferon, was described by E. F. Wheelock ...
(IFN-γ) released from sensitized
lymphocytes A lymphocyte is a type of white blood cell (leukocyte) in the immune system of most vertebrates. Lymphocytes include natural killer cells (which function in cell-mediated, cytotoxic innate immunity), T cells (for cell-mediated, cytotoxic adap ...
in whole blood incubated overnight with purified
protein Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues. Proteins perform a vast array of functions within organisms, including catalysing metabolic reactions, DNA replication, respo ...
derivative (PPD) from ''M. tuberculosis'' and control antigens.
Tuberculin Tuberculin, also known as purified protein derivative, is a combination of proteins that are used in the diagnosis of tuberculosis. This use is referred to as the tuberculin skin test and is recommended only for those at high risk. Reliable admi ...
skin testing (TST) has been used for years as an aid in diagnosing latent tuberculosis infection (LTBI) and includes measurement of the delayed type
hypersensitivity Hypersensitivity (also called hypersensitivity reaction or intolerance) refers to undesirable reactions produced by the normal immune system, including allergies and autoimmunity. They are usually referred to as an over-reaction of the immune s ...
response 48–72 hours after
intradermal Intradermal injection, often abbreviated ID, is a shallow or superficial injection of a substance into the dermis, which is located between the epidermis and the hypodermis. For certain substances, administration via an ID route can result in a ...
injection of PPD. TST and QFT do not measure the same components of the immunologic response and are not interchangeable. Assessment of the accuracy of these tests is limited by lack of a standard for confirming LTBI. As a diagnostic test, QFT: # requires
phlebotomy Phlebotomy is the process of making a puncture in a vein, usually in the arm, with a cannula for the purpose of drawing blood. The procedure itself is known as a venipuncture, which is also used for intravenous therapy. A person who performs a p ...
# can be accomplished after a single patient visit # assesses responses to multiple antigens simultaneously # does not boost anamnestic immune responses (see Latent tuberculosis#Boosting). Compared with TST, QFT results are less subject to reader bias and error. In a CDC-sponsored multicenter trial, QFT and TST results were moderately concordant (overall kappa value = 0.60). The level of concordance was adversely affected by prior bacille Calmette-Guérin (BCG) vaccination, immune reactivity to nontuberculous mycobacteria (NTM), and a prior positive TST. In addition to the multicenter study, two other published studies have demonstrated moderate concordance between TST and QFT. However, one of the five sites involved in the CDC study reported less agreement. Although there have been studies confirming the increased future risk of active TB in individuals with positive TST, the same was not true for those with a positive IGRA result. A recently published study demonstrated that a positive IGRA result is predictive of future active TB risk. Moreover, IGRA was at least as sensitive and was more specific compared to traditional TST. In this study of immunocompetent recently exposed close contacts of active TB cases, the progression rate to active disease among untreated QFT positive individuals was significantly greater than for untreated TST positives (14.6% versus 2.3%). Although the numbers were small, all of the close contacts who went on to develop active TB were QFT positive, but only 83% were TST positive. As noted above, prior BCG vaccination can produce false positive TST results. In a study of military personnel returning from missions, about one-half of the positive TSTs were falsely positive. In a more recent study of military returning from missions, Franken et al. reported evidence suggesting false positive TST results are common and that QFT testing could guide more targeted treatment and alleviate unnecessary anti-tuberculous treatment. The FDA's cutpoint for a positive result was established at >0.34 International Units/millilitre (IU/ml), though this has proven functionally problematic in low prevalence areas, such as among US and Canadian healthcare workers. In areas of low risk and low prevalence, the positive predictive value of any test is diminished. In the case of serially screened North American healthcare workers, QFT results just above this cutpoint produce false-positive test results that upon repeat testing revert to negative, where tuberculosis screening is often mandated on an annual basis. Research at Stanford University and the Veterans Administration has reported the use of a retesting (or borderline) zone below 1.1 IU/ml mitigates 76% of the false-positives, or reversions. Limitations of QFT include the need to draw blood and process it within 16 hours after collection and limited laboratory and clinical experience with the assay. There is need for further study of the utility of QFT in predicting the progression to active tuberculosis, particularly in children and immunocompromised hosts. To its disadvantage, QFT can yield false positive results with '' Mycobacterium szulgai'', ''
Mycobacterium kansasii ''Mycobacterium kansasii'' is a bacterium in the ''Mycobacterium'' genus. It is an environmental bacteria that causes opportunistic infections in humans, and is the one of the leading mycobacterial causes of human disease after tuberculosis and ...
'', and ''
Mycobacterium marinum ''Mycobacterium marinum'' is a slow growing mycobacterium (SGM) belonging to the genus ''Mycobacterium'' and the phylum Actinobacteria. The strain marinum was first identified by Aronson in 1926 and it is observed as a pathogenic mycobacterium. F ...
''.


QuantiFERON-TB Gold

The QuantiFERON-TB Gold test (QFT-G) is a whole-blood test for use as an aid in diagnosing ''Mycobacterium tuberculosis'' infection, including latent tuberculosis infection (LTBI) and tuberculosis (TB) disease. This test was approved by the U.S. Food and Drug Administration (FDA) in 2005. Blood samples are mixed with antigens (substances that can produce an immune response) and controls. For QFT-G, the antigens include mixtures of synthetic
peptide Peptides (, ) are short chains of amino acids linked by peptide bonds. Long chains of amino acids are called proteins. Chains of fewer than twenty amino acids are called oligopeptides, and include dipeptides, tripeptides, and tetrapeptides. A ...
s representing two ''M. tuberculosis'' proteins,
ESAT-6 ESAT-6 or Early Secreted Antigenic Target 6 kDa, is produced by ''Mycobacterium tuberculosis'', it is a secretory protein and potent T cell antigen. It is used in tuberculosis diagnosis by the whole blood interferon γ test QuantiFERON-TB Gold, i ...
and CFP-10. After incubation of the blood with antigens for 16 to 24 hours, the amount of interferon-gamma (IFN-gamma) is measured. If the patient is infected with ''M. tuberculosis'', their
white blood cell White blood cells, also called leukocytes or leucocytes, are the cell (biology), cells of the immune system that are involved in protecting the body against both infectious disease and foreign invaders. All white blood cells are produced and de ...
s will release IFN-gamma in response to contact with the TB antigens. The QFT-G results are based on the amount of IFN-gamma that is released in response to the antigens. Clinical evaluation and additional tests (such as a
chest radiograph A chest radiograph, called a chest X-ray (CXR), or chest film, is a projection radiograph of the chest used to diagnose conditions affecting the chest, its contents, and nearby structures. Chest radiographs are the most common film taken in med ...
, sputum smear, and culture) are needed to differentiate between a diagnosis of latent TB or active TB. Advantages of the test are: * Requires a single patient visit to draw a blood sample. * Results can be available within 24 hours. * Does not boost responses measured by subsequent tests, which can happen with tuberculin skin tests (TST). * Is not subject to reader bias that can occur with TST. * Is not affected by prior BCG (bacille Calmette-Guérin) vaccination. Disadvantages and limitations of the test are: * Blood samples must be processed within 16 hours after collection while white blood cells are still viable. * There is limited data on the use of QFT-G in children younger than 17 years of age, among persons recently exposed to ''M. tuberculosis'', and in immunocompromised persons (e.g., impaired immune function caused by HIV infection or acquired immunodeficiency syndrome
IDS IDS may refer to: Computing * IBM Informix Dynamic Server, a relational database management system * Ideographic Description Sequence, describing a Unihan character as a combination of other characters * Integrated Data Store, one of the first da ...
current treatment with immunosuppressive drugs, selected hematological disorders, specific malignancies, diabetes, silicosis, and chronic
kidney failure Kidney failure, also known as end-stage kidney disease, is a medical condition in which the kidneys can no longer adequately filter waste products from the blood, functioning at less than 15% of normal levels. Kidney failure is classified as eit ...
). * Errors in collecting or transporting blood specimens or in running and interpreting the assay can decrease the accuracy of QFT-G. * Variability, a big issue that influences the result adversely. More automation will be needed to accurately measure the QFT response. * Limited data on the use of QFT-G to determine who is at risk for developing TB disease.


QuantiFERON-TB Gold In-Tube

On 10/10/2007 the US FDA gave approval for the Quantiferon TB Gold In Tube to be marketed in the US The FDA states:
''Approval for a modification of the quantiferon-tb gold to an in-tube collection system that consists of three blood collection tubes, nil, tb antigen, and mitogen. The device, as modified, will be marketed under the trade name quantiferon-tb gold in-tube and is indicated for use as an in vitro diagnostic test using a peptide cocktail simulating esat-6, cfp-10 and tb 7.7(p4) proteins to stimulate cells in heparinized whole blood drawn directly into specialized blood collection tubes. Detection of interferon-y by enzyme-linked immunosorbent assay (elisa) is used to identify in vitro responses to these peptide antigens that are associated with mycobacterium tuberculosis infection.''
According to the FDA approved package insert Quantiferon TB Gold In Tube has a consistent specificity of >99% in low risk individuals and a sensitivity as high as 92% in individuals with active disease, depending on setting and extent of disease. The specificity in two studies of a few hundred people is 96-98% in a health immunised population. The package insert also advises that the kit provides three collection tubes which have had antigens dried onto their walls and that these tubes must be transferred to an incubator within 16 hours of blood collection. On 25 June 2010, the US Centers for Disease Control and Prevention (CDC) updated the tuberculosis (TB) testing guidelines providing guidance to US public health officials, clinicians, and laboratory workers regarding screening for and diagnosis of TB infection. The updated guidelines provide new direction for TB control in the US. Previously, QuantiFERON®-TB Gold was able to be used in any situation in which the Tuberculin Skin Test (TST) was used, without preference. The 2010 guidelines establish a new benchmark because they recommend IGRAs as the preferred TB testing method in many patients, including those who are BCG vaccinated or are unlikely to return for TST reading.


Availability

In the United States, the test is widely available from state public health laboratories, hospitals, and commercial laboratories. In January 2008 the CDC advised - via their TB Notes Newsletter - TB controllers and others of a link to a list of laboratories in the US and Canada offering to perform the Quantiferon Gold test. The California Tuberculosis Controllers Association have also provided a list of public health laboratories in California that are testing with Quantiferon.


References

{{Tuberculosis Tuberculosis