NOBOX

Homeobox protein NOBOX, also known as newborn ovary homeobox protein, is a protein that in humans is encoded by the ''NOBOX'' gene. The official symbol (NOBOX) and the official full name (NOBOX oogenesis homeobox) are maintained by the HGNC. The ''NOBOX'' gene is conserved in chimpanzee, Rhesus monkey, cow, mouse, and rat. There are 175 organisms that have orthologs with human gene ''NOBOX.'' It is capable of regulating other genes that are important in the development of follicles. Follicles do not develop and oocytes decrease in its absence which lead to infertility.

Discovery

NOBOX is an ''in silico'' subtraction discovery when Suzumori ''et al''. searched for novel genes involved in early mammalian folliculogenesis in 2002. It is one of the several genes that appeared in the search in expressed sequence tag (EST) databases of mouse. It was then cloned and characterised for its genomic structure.

Gene location

The human ''NOBOX'' is located in chromosome 7q35 while the mouse ''NOBOX'' is in proximal chromosome 6.

Protein structure

The human ''NOBOX'' is a 14 kb protein and encoded by 8 exons. It has a proline rich C terminus and contains putative SH3 and WW domains. This C terminus is believed to be critical in its transcriptional activities when bound to oocyte-specific genes. NOBOX belongs to the family of proteins that contains homeodomain. Homeodomain is a stretch of 32 specific amino acids in primates downstream the NOBOX Arg303 residue and is very well-conserved among the species. It contains an asparagine residue at position 51 which is important for its interactions with DNA base pairs.

Function

''NOBOX'' is a homeobox gene that is preferentially expressed in oocytes. In mice, it is essential for folliculogenesis and regulation of oocyte-specific genes. Regulation of these oocyte-specific genes is thru direct binding of NOBOX to its promoter regions via the specific consensus sequences, the NOBOX DNA binding elements (NBEs). There are three NBEs that have been identified: 5'-TAATTG-3', 5'-TAGTTG-3', and 5'-TAATTA-3'. Knockout study of ''NOBOX'' against wild-type ovaries in newborn female mice revealed that 74% (28/38 genes) were downregulated more than 5-fold and 15% (5/33 genes) were upregulated more than 5-fold. However, microRNA population is not affected by ''NOBOX'' in newborn ovaries. NOBOX also plays an important role in the suppression of male-determining genes such as ''DMRT1, Dmrt1''. Its deficiency can cause rapid loss of postnatal oocytes and during its absence in female mice, follicles are replaced by fibrous tissue. Recently, a new role of NOBOX in controlling the G2-M DNA damage checkpoint, G2/M arrest was discovered.

Mutations and clinical significance

A mutation in the NOBOX gene is associated with premature ovarian failure, premature ovarian failure (POF), also known as premature ovarian insufficiency (POI). It is a condition which ovaries loss its normal function before the age of 40. It is a heritable disease in up to 30% of patients which is characterised by secondary infertility, amenorrhea, hypoestrogenism, and elevated follicle-stimulating hormone levels in the serum (FSH>40IU/liter). It affects ≈1% of women below 40 years old. A study conducted on 96 white women with POF revealed one case of Zygosity, heterozygous mutation in the NOBOX homeodomain, p.Arg355His, in one patient. This mutation was absent in the control population and significantly disrupts the binding of NOBOX to the NBE. Arg355 is critical to DNA binding and is conserved in the homeodomain of the NOBOX from zebrafish to humans. Moreover, its significant negative effect suggests that NOBOX homeodomain may function as a dimer but its rare occurrence suggests a low contribution to POF. Further investigations on POF were conducted on Caucasian, African, Chinese, and Japanese women diagnosed with POF. Several NOBOX loss-of-function mutations were observed in Caucasian and African women accounting to 6.2%, 5.6% and 6.4%. These results suggest that ''NOBOX'' gene is a strong autosomal candidate for POF and its genetic mechanism involves haploinsufficiency. However, these mutations were not found in Chinese and Japanese women making it a less common explanation for POF in the region.

The POF syndrome is a highly heterogenous clinical disorder but a recent study showed the first Zygosity, homozygous mutation associated with NOBOX loss-of-function. One patient out of 96 population diagnosed with POF in China was found with one novel homozygous truncating variant in the ''NOBOX'' gene. This truncated variant caused a defective transcriptional activation of Growth differentiation factor-9, GDF9, a well-known target of NOBOX, which led to the lost ability of NOBOX to induce G2-M DNA damage checkpoint, G2/M arrest. This finding disagrees that mutation is a less common explanation for POF in Asian population.

Understanding the mutations in NOBOX homeodomain is important to researchers and clinicians to develop diagnostic and therapeutic approaches for POF such as genetic control of mammalian reproductive life-span, regulation of fertility, and generation of mature eggs in the lab.

Interactions

# Bone morphogenetic protein 15, GDF9
# Oct-4, POU5F1
# DNMT1, DNMT10
# Forkhead box L2, FOXL2
#FIGLA
#R-spondin 2, RSPO2
#DMRT1

References

Further reading

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{{Transcription factors, g3

Transcription factors