The immobilized whole cell system is an alternative to

enzyme immobilization An immobilized enzyme is an enzyme, with restricted mobility, attached to an inert, insoluble material—such as calcium alginate (produced by reacting a mixture of sodium alginate solution and enzyme solution with calcium chloride). This can prov ...

. Unlike enzyme immobilization, where the enzyme is attached to a solid support (such as calcium alginate or activated PVA or activated PEI), in immobilized whole cell systems, the target cell is immobilized. Such methods may be implemented when the enzymes required are difficult or expensive to extract, an example being

intracellular This glossary of biology terms is a list of definitions of fundamental terms and concepts used in biology, the study of life and of living organisms. It is intended as introductory material for novices; for more specific and technical definitions ...

enzymes. Also, if a series of enzymes are required in the reaction; whole cell immobilization may be used for convenience. This is only done on a commercial basis when the need for the product is more justified. Multiple enzymes may be introduced into the reaction, thus eliminating the need for immobilization of multiple enzymes. Furthermore, intracellular enzymes need not be extracted prior to the reaction; they may be used directly. However, some enzymes may be used for the metabolic needs of the cell, leading to reduced yield of the cell.

References

See also