RLS may refer to:

Education

* Royal Latin School, a grammar school in Buckingham, England * Stevenson School, formerly Robert Louis Stevenson School, California

Science and technology

* Recursive least squares filter, in minimisation * Regularized least squares, for solving least-squares problems * Reef Life Survey, a marine monitoring program in Tasmania * Restless legs syndrome, a medical disorder

* Real-life slash, a subgenre of real person fiction * Rial (disambiguation), plural abbreviation Rls, Middle Eastern currency units * Robert Louis Stevenson, Scottish writer {{disambiguation