Coronaviruses are a group of related RNA viruses that cause diseases in mammals and birds. In humans and birds, they cause respiratory tract infections that can range from mild to lethal. Mild illnesses in humans include some cases of the common cold (which is also caused by other viruses, predominantly rhinoviruses), while more lethal varieties can cause SARS, MERS and COVID-19, which is causing the COVID-19 pandemic, ongoing pandemic. In cows and pigs they cause diarrhea, while in mice they cause hepatitis and encephalomyelitis. Coronaviruses constitute the subfamily ''Orthocoronavirinae'', in the family ''Coronaviridae'', order ''Nidovirales'' and realm ''Riboviria''. They are enveloped viruses with a Positive-strand RNA virus, positive-sense single-stranded RNA genome and a nucleocapsid of helical symmetry. The genome size of coronaviruses ranges from approximately 26 to 32 kilobases, one of the largest among RNA viruses. They have characteristic club-shaped Spike protein, spikes that project from their surface, which in electron micrographs create an image reminiscent of the stellar corona, from which their name derives.

Etymology

The name "coronavirus" is derived from Latin ''wiktionary:corona#Latin, corona'', meaning "crown" or "wreath", itself a borrowing from Ancient Greek, Greek ''korṓnē'', "garland, wreath". The name was coined by June Almeida and David Tyrrell (physician), David Tyrrell who first observed and studied human coronaviruses. The word was first used in print in 1968 by an informal group of virologists in the journal ''Nature (journal), Nature'' to designate the new family of viruses. The name refers to the characteristic appearance of virions (the infective form of the virus) by electron microscopy, which have a fringe of large, bulbous surface projections creating an image reminiscent of the solar corona or halo. This Morphology (biology), morphology is created by the viral spike peplomers, which are proteins on the surface of the virus. The scientific name ''Coronavirus'' was accepted as a genus name by the International Committee for the Nomenclature of Viruses (later renamed International Committee on Taxonomy of Viruses) in 1971. As the number of new species increased, the genus was split into four genera, namely ''Alphacoronavirus'', ''Betacoronavirus'', ''Deltacoronavirus (genus), Deltacoronavirus'', and ''Gammacoronavirus'' in 2009. The common name coronavirus is used to refer to any member of the subfamily ''Orthocoronavirinae''. As of 2020, 45 species are officially recognised.

History

The earliest reports of a coronavirus infection in animals occurred in the late 1920s, when an acute respiratory infection of domesticated chickens emerged in North America. Arthur Schalk and M.C. Hawn in 1931 made the first detailed report which described a new Avian infectious bronchitis, respiratory infection of chickens in North Dakota. The infection of new-born chicks was characterized by gasping and listlessness with high mortality rates of 40–90%. Leland David Bushnell and Carl Alfred Brandly isolated the virus that caused the infection in 1933. The virus was then known as Avian coronavirus, infectious bronchitis virus (IBV). Charles D. Hudson and Fred Robert Beaudette cultivated the virus for the first time in 1937. The specimen came to be known as the Beaudette strain. In the late 1940s, two more animal coronaviruses, JHM that causes brain disease (murine encephalitis) and Murine coronavirus, mouse hepatitis virus (MHV) that causes hepatitis in mice were discovered. It was not realized at the time that these three different viruses were related. Human coronaviruses were discovered in the 1960s using two different methods in the United Kingdom and the United States. E.C. Kendall, Malcolm Bynoe, and David Tyrrell (physician), David Tyrrell working at the Common Cold Unit of the Medical Research Council (United Kingdom), British Medical Research Council collected a unique common cold virus designated B814 in 1961. The virus could not be cultivated using standard techniques which had successfully cultivated rhinoviruses, Adenoviridae, adenoviruses and other known common cold viruses. In 1965, Tyrrell and Bynoe successfully cultivated the novel virus by Serial passage, serially passing it through organ culture of Embryo, human embryonic trachea. The new cultivating method was introduced to the lab by Bertil Hoorn. The isolated virus when intranasally Inoculation, inoculated into volunteers caused a cold and was inactivated by ether which indicated it had a Viral envelope, lipid envelope. Dorothy Hamre and John Procknow at the University of Chicago isolated a novel cold from medical students in 1962. They isolated and grew the virus in kidney tissue culture, designating it 229E. The novel virus caused a cold in volunteers and, like B814, was inactivated by ether. TEM of coronavirus OC43

Scottish people, Scottish virologist June Almeida at St Thomas' Hospital in London, collaborating with Tyrrell, compared the structures of IBV, B814 and 229E in 1967. Using Transmission electron microscopy, electron microscopy the three viruses were shown to be morphologically related by their general shape and distinctive club-like Peplomer, spikes. A research group at the National Institutes of Health, National Institute of Health the same year was able to isolate another member of this new group of viruses using organ culture and named one of the samples OC43 (OC for organ culture). Like B814, 229E, and IBV, the novel cold virus OC43 had distinctive club-like spikes when observed with the electron microscope. The IBV-like novel cold viruses were soon shown to be also morphologically related to the mouse hepatitis virus. This new group of viruses were named coronaviruses after their distinctive morphological appearance. Human coronavirus 229E and human coronavirus OC43 continued to be studied in subsequent decades. The coronavirus strain B814 was lost. It is not known which present human coronavirus it was. Other human coronaviruses have since been identified, including Severe acute respiratory syndrome coronavirus, SARS-CoV in 2003, Human coronavirus NL63, HCoV NL63 in 2003, Human coronavirus HKU1, HCoV HKU1 in 2004, Middle East respiratory syndrome-related coronavirus, MERS-CoV in 2013, and SARS-CoV-2 in 2019. There have also been a large number of animal coronaviruses identified since the 1960s.''''

Microbiology

Structure

Coronaviruses are large, roughly spherical particles with unique surface projections. Their size is highly variable with average diameters of 80 to 120 Nanometre, nm. Extreme sizes are known from 50 to 200 nm in diameter. The total molecular mass is on average 40,000 Dalton (unit), kDa. They are enclosed in an envelope embedded with a number of protein molecules. The lipid bilayer envelope, membrane proteins, and nucleocapsid protect the virus when it is outside the host cell. The viral envelope is made up of a lipid bilayer in which the coronavirus membrane protein, membrane (M), coronavirus envelope protein, envelope (E) and spike protein, spike (S) Viral structural protein, structural proteins are anchored. The molar ratio of E:S:M in the lipid bilayer is approximately 1:20:300. The E and M protein are the structural proteins that combined with the lipid bilayer to shape the viral envelope and maintain its size. S proteins are needed for interaction with the host cells. But human coronavirus NL63 is peculiar in that its M protein has the binding site for the host cell, and not its S protein. The diameter of the envelope is 85 nm. The envelope of the virus in electron micrographs appears as a distinct pair of electron-dense shells (shells that are relatively opaque to the electron beam used to scan the virus particle). The coronavirus membrane protein, M protein is the main structural protein of the envelope that provides the overall shape and is a Bitopic protein, type III membrane protein. It consists of 218 to 263 Amino acid residues and forms a layer 7.8 nm thick. It has three domains, a short N-terminus, N-terminal ectodomain, a triple-spanning transmembrane domain, and a C-terminus, C-terminal Endoplasm, endodomain. The C-terminal domain forms a matrix-like lattice that adds to the extra-thickness of the envelope. Different species can have either ''N''- or ''O''-linked glycans in their protein amino-terminal domain. The M protein is crucial during the assembly, budding, envelope formation, and pathogenesis stages of the virus lifecycle. The coronavirus envelope protein, E proteins are minor structural proteins and highly variable in different species. There are only about 20 copies of the E protein molecule in a coronavirus particle. They are 8.4 to 12 kDa in size and are composed of 76 to 109 amino acids. They are integral proteins (i.e. embedded in the lipid layer) and have two domains namely a transmembrane domain and an extramembrane C-terminal domain. They are almost fully α-helical, with a single α-helical transmembrane domain, and form pentameric (five-molecular) ion channels in the lipid bilayer. They are responsible for virion assembly, Intracellular transport, intracellular trafficking and morphogenesis (budding). SARS-CoV MERS-CoV genome organization and S-protein domains

SARS-CoV MERS-CoV genome organization and S-protein domains

The spikes are the most distinguishing feature of coronaviruses and are responsible for the corona- or halo-like surface. On average a coronavirus particle has 74 surface spikes. Each Peplomer, spike is about 20 nm long and is composed of a Protein trimer, trimer of the Sprotein. The S protein is in turn composed of an S1 and S2 Protein domain, subunit. The homotrimeric Sprotein is a Membrane fusion protein, class I fusion protein which mediates the Viral entry, receptor binding and Lipid bilayer fusion, membrane fusion between the virus and host cell. The S1 subunit forms the head of the spike and has the receptor-binding domain (RBD). The S2 subunit forms the stem which anchors the spike in the viral envelope and on protease activation enables fusion. The two subunits remain noncovalently linked as they are exposed on the viral surface until they attach to the host cell membrane. In a functionally active state, three S1 are attached to two S2 subunits. The subunit complex is split into individual subunits when the virus binds and fuses with the host cell under the action of proteases such as cathepsin family and TMPRSS2, transmembrane protease serine 2 (TMPRSS2) of the host cell. 15010_2020_1486_Fig3_HTML

S1 proteins are the most critical components in terms of infection. They are also the most variable components as they are responsible for host cell specificity. They possess two major domains named N-terminal domain (S1-NTD) and C-terminal domain (S1-CTD), both of which serve as the receptor-binding domains. The NTDs recognize and bind sugars on the surface of the host cell. An exception is the Murine coronavirus, MHV NTD that binds to a protein receptor carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1). S1-CTDs are responsible for recognizing different protein receptors such as angiotensin-converting enzyme 2 (ACE2), aminopeptidase N (APN), and dipeptidyl peptidase 4 (DPP4). A subset of coronaviruses (specifically the members of betacoronavirus Embecovirus, subgroup A) also has a shorter spike-like surface protein called hemagglutinin esterase (HE). The HE proteins occur as homodimers composed of about 400 amino acid residues and are 40 to 50 kDa in size. They appear as tiny surface projections of 5 to 7 nm long embedded in between the spikes. They help in the attachment to and detachment from the host cell. Inside the envelope, there is the Capsid, nucleocapsid, which is formed from multiple copies of the nucleocapsid (N) protein, which are bound to the positive-sense single-stranded RNA genome in a continuous Beads-on-a-string type conformation. Coronavirus nucleocapsid protein, N protein is a phosphoprotein of 43 to 50 kDa in size, and is divided into three conserved domains. The majority of the protein is made up of domains 1 and 2, which are typically rich in arginines and lysines. Domain 3 has a short carboxy terminal end and has a net negative charge due to excess of acidic over basic amino acid residues.

Genome

Coronaviruses contain a Positive-sense single-stranded RNA virus, positive-sense, single-stranded RNA genome. The genome size for coronaviruses ranges from 26.4 to 31.7 Kilobase#Length measurements, kilobases. The genome size is one of the largest among RNA viruses. The genome has a Five-prime cap, 5′ methylated cap and a Polyadenylation, 3′ polyadenylated tail. The genome organization for a coronavirus is Five prime untranslated region, 5′-leader-UTR-replicase (ORF1ab)-spike (S)-envelope (E)-membrane (M)-nucleocapsid (N)-Three prime untranslated region, 3′UTR-poly (A) tail. The open reading frames 1a and 1b, which occupy the first two-thirds of the genome, encode the replicase polyprotein (pp1ab). The replicase polyprotein self cleaves to form 16 Viral nonstructural protein, nonstructural proteins (nsp1–nsp16). The later reading frames encode the four major structural proteins: spike protein, spike, coronavirus envelope protein, envelope, coronavirus envelope protein, membrane, and coronavirus nucleocapsid protein, nucleocapsid. Interspersed between these reading frames are the reading frames for the accessory proteins. The number of accessory proteins and their function is unique depending on the specific coronavirus.

Replication cycle

Cell entry

Infection begins when the viral spike protein attaches to its complementary host cell receptor. After attachment, a protease of the host cell Proteolysis, cleaves and activates the receptor-attached spike protein. Depending on the host cell protease available, cleavage and activation allows the Viral entry, virus to enter the host cell by endocytosis or direct fusion of the viral envelope with the Lipid bilayer, host membrane.

Genome translation

On entry into the Host (biology), host cell, the virus particle is Uncoating, uncoated, and its genome enters the Cytoplasm, cell cytoplasm. The coronavirus RNA genome has a 5′ methylated cap and a 3′ polyadenylated tail, which allows it to act like a messenger RNA and be directly translated by the host cell's ribosomes. The host ribosomes translate the initial overlapping Reading frame, open reading frames ORF1a and ORF1b of the virus genome into two large overlapping polyproteins, pp1a and pp1ab. The larger polyprotein pp1ab is a result of a Ribosomal frameshift, -1 ribosomal frameshift caused by a slippery sequence (UUUAAAC) and a downstream Nucleic acid secondary structure, RNA pseudoknot at the end of open reading frame ORF1a. The ribosomal frameshift allows for the continuous translation of ORF1a followed by ORF1b. The polyproteins have their own C30 Endopeptidase, proteases, Papain, PLpro (nsp3) and C30 Endopeptidase, 3CLpro (nsp5), which cleave the polyproteins at different specific sites. The cleavage of polyprotein pp1ab yields 16 nonstructural proteins (nsp1 to nsp16). Product proteins include various replication proteins such as RNA-dependent RNA polymerase (nsp12), RNA helicase (nsp13), and exoribonuclease (nsp14).

Replicase-transcriptase

Replication-transcription complex for Coronaviruses cropped

A number of the nonstructural proteins coalesce to form a Protein complex, multi-protein replicase-transcriptase complex (RTC). The main replicase-transcriptase protein is the RNA-dependent RNA polymerase (RdRp). It is directly involved in the DNA replication, replication and Transcription (biology), transcription of RNA from an RNA strand. The other nonstructural proteins in the complex assist in the replication and transcription process. The exoribonuclease nonstructural protein, for instance, provides extra fidelity to replication by providing a Proofreading (biology), proofreading function which the RNA-dependent RNA polymerase lacks. ''Replication'' – One of the main functions of the complex is to replicate the viral genome. RdRp directly mediates the DNA replication, synthesis of negative-sense genomic RNA from the positive-sense genomic RNA. This is followed by the replication of positive-sense genomic RNA from the negative-sense genomic RNA. Transcription of nested mRNAs

''Transcription'' – The other important function of the complex is to transcribe the viral genome. RdRp directly mediates the Transcription (biology), synthesis of negative-sense subgenomic RNA molecules from the positive-sense genomic RNA. This process is followed by the transcription of these negative-sense subgenomic RNA molecules to their corresponding positive-sense Messenger RNA, mRNAs. The subgenomic mRNAs form a "Subgenomic mRNA, nested set" which have a common 5'-head and partially duplicate 3'-end. ''Recombination'' – The replicase-transcriptase complex is also capable of genetic recombination when at least two viral genomes are present in the same infected cell. RNA recombination appears to be a major driving force in determining genetic variability within a coronavirus species, the capability of a coronavirus species to jump from one host to another and, infrequently, in determining the emergence of novel coronaviruses. The exact mechanism of recombination in coronaviruses is unclear, but likely involves template switching during genome replication.

Assembly and release

The replicated positive-sense genomic RNA becomes the genome of the Viral shedding, progeny viruses. The mRNAs are gene transcripts of the last third of the virus genome after the initial overlapping reading frame. These mRNAs are translated by the host's ribosomes into the structural proteins and many accessory proteins. RNA translation occurs inside the endoplasmic reticulum. The viral structural proteins S, E, and M move along the secretory pathway into the Vesicular-tubular cluster, Golgi intermediate compartment. There, the Mproteins direct most protein-protein interactions required for the assembly of viruses following its binding to the nucleocapsid. Progeny viruses are then released from the host cell by exocytosis through secretory vesicles. Once released the viruses can infect other host cells.

Transmission

Infected carriers are able to Transmission (medicine), shed viruses into the environment. The interaction of the coronavirus spike protein with its complementary Viral entry, cell receptor is central in determining the tissue tropism, infectivity, and Host tropism, species range of the released virus. Coronaviruses mainly target Epithelium, epithelial cells.'''' They are transmitted from one host to another host, depending on the coronavirus species, by either an Bioaerosol, aerosol, fomite, or Fecal–oral route, fecal-oral route. Human coronaviruses infect the epithelial cells of the respiratory tract, while animal coronaviruses generally infect the epithelial cells of the Gastrointestinal tract, digestive tract.'''' Severe acute respiratory syndrome–related coronavirus, SARS coronavirus, for example, infects the human epithelial cells of the lungs via an aerosol route by binding to the angiotensin-converting enzyme 2 (ACE2) receptor. Transmissible gastroenteritis virus, Transmissible gastroenteritis coronavirus (TGEV) infects the pig epithelial cells of the digestive tract via a fecal-oral route by binding to the Alanine aminopeptidase (APN) receptor.

Classification

Coronaviruses form the subfamily ''Orthocoronavirinae,'' which is one of two sub-families in the family ''Coronaviridae,'' order ''Nidovirales,'' and realm ''Riboviria.'' They are divided into the four genera: ''Alphacoronavirus'', ''Betacoronavirus'', ''Gammacoronavirus'' and ''Deltacoronavirus''. Alphacoronaviruses and betacoronaviruses infect mammals, while gammacoronaviruses and deltacoronaviruses primarily infect birds.' * Genus: ''Alphacoronavirus''; ** Species: ''Alphacoronavirus 1'' (Transmissible gastroenteritis virus, TGEV, Feline coronavirus, Canine coronavirus), ''Human coronavirus 229E'', ''Human coronavirus NL63'', ''Miniopterus bat coronavirus 1'', ''Miniopterus bat coronavirus HKU8'', ''Porcine epidemic diarrhea virus'', ''Rhinolophus bat coronavirus HKU2'', ''Scotophilus bat coronavirus 512'' * Genus ''Betacoronavirus''; ** Species: ''Betacoronavirus 1'' (Bovine coronavirus, ''Bovine Coronavirus'', ''Human coronavirus OC43''), ''Hedgehog coronavirus 1,'' ''Human coronavirus HKU1'', ''Middle East respiratory syndrome-related coronavirus,'' ''Murine coronavirus'', ''Pipistrellus bat coronavirus HKU5'', ''Rousettus bat coronavirus HKU9'', ''Severe acute respiratory syndrome–related coronavirus'' (''SARS-CoV'', ''SARS-CoV-2''), ''Tylonycteris bat coronavirus HKU4'' * Genus ''Gammacoronavirus''; ** Species: ''Avian coronavirus,'' ''Beluga whale coronavirus SW1'' * Genus ''Deltacoronavirus (genus), Deltacoronavirus'' ** Species: ''Bulbul coronavirus HKU11'', Porcine coronavirus HKU15, ''Porcine'' ''coronavirus HKU15''

Origin

The most recent common ancestor (MRCA) of all coronaviruses is estimated to have existed as recently as 8000 BCE, although some models place the common ancestor as far back as 55 million years or more, implying long term coevolution with bat and avian species. The most recent common ancestor of the alphacoronavirus line has been placed at about 2400 BCE, of the betacoronavirus line at 3300 BCE, of the gammacoronavirus line at 2800 BCE, and the deltacoronavirus line at about 3000 BCE. Bats and birds, as warm-blooded flying vertebrates, are an ideal natural reservoir for the coronavirus gene pool (with Bat-borne virus, bats the reservoir for alphacoronaviruses and betacoronavirusand birds the reservoir for gammacoronaviruses and deltacoronaviruses). The large number and global range of bat and avian species that host viruses have enabled extensive evolution and dissemination of coronaviruses. Many human coronaviruses have their origin in bats. The human coronavirus NL63 shared a common ancestor with a bat coronavirus (ARCoV.2) between 1190 and 1449 CE. The human coronavirus 229E shared a common ancestor with a bat coronavirus (GhanaGrp1 Bt CoV) between 1686 and 1800 CE. More recently, alpaca coronavirus and human coronavirus 229E diverged sometime before 1960. MERS-CoV emerged in humans from bats through the intermediate host of camels. MERS-CoV, although related to several bat coronavirus species, appears to have diverged from these several centuries ago. The most closely related bat coronavirus and SARS-CoV diverged in 1986. The ancestors of SARS-CoV first infected leaf-nose bats of the genus ''Hipposideridae''; subsequently, they spread to horseshoe bats in the species ''Rhinolophidae'', then to Asian palm civets, and finally to humans. Unlike other betacoronaviruses, bovine coronavirus of the species ''Betacoronavirus 1'' and subgenus ''Embecovirus'' is thought to have originated in rodents and not in bats. In the 1790s, equine coronavirus diverged from the bovine coronavirus after a Cross-species transmission, cross-species jump. Later in the 1890s, human coronavirus OC43 diverged from bovine coronavirus after another cross-species spillover event. It is speculated that the 1889–1890 flu pandemic, flu pandemic of 1890 may have been caused by this spillover event, and not by the Orthomyxoviridae, influenza virus, because of the related timing, neurological symptoms, and unknown causative agent of the pandemic. Besides causing respiratory infections, human coronavirus OC43 is also suspected of playing a role in Demyelinating disease, neurological diseases. In the 1950s, the human coronavirus OC43 began to diverge into its present genotypes. Phylogenetically, mouse hepatitis virus (''Murine coronavirus''), which infects the mouse's liver and Central nervous system viral disease, central nervous system, is related to human coronavirus OC43 and bovine coronavirus. Human coronavirus HKU1, like the aforementioned viruses, also has its origins in rodents.

Infection in humans

Coronaviruses vary significantly in risk factor. Some can kill more than 30% of those infected, such as Middle East respiratory syndrome-related coronavirus, MERS-CoV, and some are relatively harmless, such as the common cold. Coronaviruses can cause colds with major symptoms, such as fever, and a sore throat from swollen adenoids. Coronaviruses can cause pneumonia (either direct viral pneumonia or secondary bacterial pneumonia) and bronchitis (either direct viral bronchitis or secondary bacterial bronchitis). The human coronavirus discovered in 2003, SARS coronavirus, SARS-CoV, which causes severe acute respiratory syndrome (SARS), has a unique pathogenesis because it causes both upper respiratory tract infection, upper and lower respiratory tract infections. Six species of human coronaviruses are known, with one species subdivided into two different strains, making seven strains of human coronaviruses altogether. Four human coronaviruses produce symptoms that are generally mild, even though it is contended they might have been more aggressive in the past: #Human coronavirus OC43 (HCoV-OC43), Betacoronavirus, β-CoV #Human coronavirus HKU1 (HCoV-HKU1), β-CoV #Human coronavirus 229E (HCoV-229E), Alphacoronavirus, α-CoV #Human coronavirus NL63 (HCoV-NL63), α-CoV– Three human coronaviruses produce potentially severe symptoms: #Severe acute respiratory syndrome coronavirus (SARS-CoV), β-CoV (identified in 2003) #Middle East respiratory syndrome-related coronavirus (MERS-CoV), β-CoV (identified in 2012) #Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), β-CoV (identified in 2019) These cause the diseases commonly called Severe acute respiratory syndrome, SARS, Middle East respiratory syndrome, MERS, and COVID-19 respectively.

Common cold

Although the common cold is usually caused by rhinoviruses, in about 15% of cases the cause is a coronavirus. The human coronaviruses HCoV-OC43, HCoV-HKU1, HCoV-229E, and HCoV-NL63 continually circulate in the human population in adults and children worldwide and produce the generally mild symptoms of the common cold. The four mild coronaviruses have a seasonal incidence occurring in the winter months in temperate climates. There is no preponderance in any season in tropical climates.

Severe acute respiratory syndrome (SARS)

In 2003, following the outbreak of severe acute respiratory syndrome (SARS) which had begun the prior year in Asia, and secondary cases elsewhere in the world, the World Health Organization (WHO) issued a press release stating that a novel coronavirus identified by several laboratories was the causative agent for SARS. The virus was officially named the SARS coronavirus (SARS-CoV). More than 8,000 people from 29 countries and territories were infected, and at least 774 died.

Middle East respiratory syndrome (MERS)

In September 2012, a new type of coronavirus was identified, initially called Novel Coronavirus 2012, and now officially named Middle East respiratory syndrome coronavirus (MERS-CoV). The World Health Organization issued a global alert soon after. The WHO update on 28 September 2012 said the virus did not seem to pass easily from person to person. However, on 12 May 2013, a case of human-to-human transmission in France was confirmed by the French Ministry of Social Affairs and Health.''Nouveau coronavirus—Point de situation : Un nouveau cas d'infection confirmé''
''(Novel coronavirus—Status report: A new case of confirmed infection)'' 12 May 2013, social-sante.gouv.fr In addition, cases of human-to-human transmission were reported by the Ministry of Health in Tunisia. Two confirmed cases involved people who seemed to have caught the disease from their late father, who became ill after a visit to Qatar and Saudi Arabia. Despite this, it appears the virus had trouble spreading from human to human, as most individuals who are infected do not transmit the virus. By 30 October 2013, there were 124 cases and 52 deaths in Saudi Arabia. After the Dutch Erasmus MC, Erasmus Medical Centre sequenced the virus, the virus was given a new name, Human Coronavirus–Erasmus Medical Centre (HCoV-EMC). The final name for the virus is Middle East respiratory syndrome coronavirus (MERS-CoV). The only U.S. cases (both survived) were recorded in May 2014. In May 2015, an outbreak of MERS-CoV occurred in the Republic of Korea, when a man who had traveled to the Middle East, visited four hospitals in the Seoul area to treat his illness. This caused one of the largest outbreaks of MERS-CoV outside the Middle East. As of December 2019, 2,468 cases of MERS-CoV infection had been confirmed by laboratory tests, 851 of which were fatal, a mortality rate of approximately 34.5%.

Coronavirus disease 2019 (COVID-19)

In December 2019, a pneumonia outbreak was reported in Wuhan, China. On 31 December 2019, the outbreak was traced to a novel strain of coronavirus, which was given the interim name 2019-nCoV by the World Health Organization, later renamed SARS-CoV-2 by the International Committee on Taxonomy of Viruses. As of , there have been at least COVID-19 pandemic deaths, confirmed deaths and more than COVID-19 pandemic cases, confirmed cases in the COVID-19 pandemic. The Wuhan strain has been identified as a new strain of Betacoronavirus from group 2B with approximately 70% genetic similarity to the SARS-CoV. The virus has a 96% similarity to a bat coronavirus, so it is widely suspected to originate from bats as well.

Coronavirus HuPn-2018

During a surveillance study of archived samples of Malaysian viral pneumonia patients, virologists identified a strain of canine coronavirus which has infected humans in 2018.

Infection in animals

Coronaviruses have been recognized as causing pathological conditions in veterinary medicine since the 1930s. They infect a range of animals including swine, cattle, horses, camels, cats, dogs, rodents, birds and bats. The majority of animal related coronaviruses infect the Gastrointestinal tract, intestinal tract and are transmitted by a fecal-oral route. Significant research efforts have been focused on elucidating the viral pathogenesis of these animal coronaviruses, especially by virologists interested in veterinary and zoonotic diseases.

Farm animals

Coronaviruses infect domesticated birds. Infectious bronchitis virus (IBV), a type of coronavirus, causes avian infectious bronchitis. The virus is of concern to the Poultry farming, poultry industry because of the high mortality from infection, its rapid spread, and its effect on production. The virus affects both meat production and egg production and causes substantial economic loss. In chickens, infectious bronchitis virus targets not only the respiratory tract but also the urogenital tract. The virus can spread to different organs throughout the chicken. The virus is transmitted by aerosol and food contaminated by feces. Different vaccines against IBV exist and have helped to limit the spread of the virus and its variants. Infectious bronchitis virus is one of a number of strains of the species ''Avian coronavirus''. Another strain of avian coronavirus is turkey coronavirus (TCV) which causes enteritis in turkeys. Coronaviruses also affect other branches of animal husbandry such as pig farming and the Cattle raising. Swine acute diarrhea syndrome coronavirus (SADS-CoV), which is related to Rhinolophus bat coronavirus HKU2, bat coronavirus HKU2, causes diarrhea in pigs. Porcine epidemic diarrhea virus (PEDV) is a coronavirus that has recently emerged and similarly causes diarrhea in pigs. Transmissible gastroenteritis virus (TGEV), which is a member of the species ''Alphacoronavirus 1'', is another coronavirus that causes diarrhea in young pigs. In the cattle industry bovine coronavirus (BCV), which is a member of the species ''Betacoronavirus 1'' and related to HCoV-OC43, is responsible for severe profuse enteritis in young calves.

Domestic pets

Coronaviruses infect domestic pets such as cats, dogs, and ferrets. There are two forms of feline coronavirus which are both members of the species ''Alphacoronavirus 1''. Feline enteric coronavirus is a pathogen of minor clinical significance, but spontaneous mutation of this virus can result in feline infectious peritonitis (FIP), a disease with high mortality. There are two different coronaviruses that infect dogs. Canine coronavirus (CCoV), which is a member of the species ''Alphacoronavirus 1'', causes mild gastrointestinal disease. Canine coronavirus#Canine respiratory coronavirus, Canine respiratory coronavirus (CRCoV), which is a member of the species ''Betacoronavirus 1'' and related to HCoV-OC43, cause respiratory disease. Similarly, there are two types of coronavirus that infect ferrets. Ferret coronavirus, Ferret enteric coronavirus causes a gastrointestinal syndrome known as epizootic catarrhal enteritis (ECE), and a more lethal systemic version of the virus (like FIP in cats) known as ferret systemic coronavirus (FSC).

Laboratory animals

Coronaviruses infect laboratory animals. Mouse hepatitis virus (MHV), which is a member of the species ''Murine coronavirus'', causes an epidemic Murinae, murine illness with high mortality, especially among colonies of laboratory mice. Prior to the discovery of SARS-CoV, MHV was the best-studied coronavirus both ''in vivo'' and ''in vitro'' as well as at the molecular level. Some strains of MHV cause a progressive Demyelinating disease, demyelinating encephalitis in mice which has been used as a murine model for multiple sclerosis. Rat coronavirus, Sialodacryoadenitis virus (SDAV), which is a strain of the species ''Murine coronavirus'', is highly infectious coronavirus of laboratory rats, which can be transmitted between individuals by direct contact and indirectly by aerosol. Rabbit enteric coronavirus causes acute gastrointestinal disease and diarrhea in young European rabbits. Mortality rates are high.

Prevention and treatment

A COVID-19 vaccine, number of vaccines using different methods have been developed against human coronavirus SARS-CoV-2. Biological target, Antiviral targets against human coronaviruses have also been identified such as viral proteases, polymerases, and entry proteins. COVID-19 drug development, Drugs are in development which target these proteins and the different steps of viral replication. Vaccines are available for animal coronaviruses IBV, TGEV, and Canine CoV, although their effectiveness is limited. In the case of outbreaks of highly contagious animal coronaviruses, such as PEDV, measures such as Culling, destruction of entire herds of pigs may be used to prevent transmission to other herds.