The epididymis (/ɛpɪˈdɪdɪmɪs/; plural: epididymides /ɛpɪdɪˈdɪmədz/ or /ɛpɪˈdɪdəmɪdz/) is a tube that connects a testicle to a vas deferens in the male reproductive system. It is present in all male reptiles, birds, and mammals. It is a single, narrow, tightly-coiled tube (in adult humans, six to seven meters in length)[1] connecting the efferent ducts from the rear of each testicle to its vas deferens.


The epididymis can be divided into three main regions:

  • The head (Latin: Caput). The head of the epididymis receives spermatozoa via the efferent ducts of the mediastinium of the testis. It is characterized histologically by a thick epithelium with long stereocilia (described below) and a little smooth muscle[2]. It is involved in absorbing fluid to make the sperm more concentrated. The concentration of the sperm here is dilute.
  • The body (Latin: Corpus). This has an intermediate epithelium and smooth muscle thickness[2].
  • The tail (Latin: Cauda). This has the thinnest epithelium of the three regions and the greatest quantity of smooth muscle[2].

In reptiles, there is an additional canal between the testis and the head of the epididymis and which receives the various efferent ducts. This is, however, absent in all birds and mammals.[3]


The epididymis is covered by a two layered pseudostratified epithelium. The epithelium is separated by a basement membrane from the connective tissue wall which has smooth muscle cells. The major cell types in the epithelium are:

  • Main cells: columnar cells that, with the basal cells, form the majority of the epithelium. These cells extend from the lumen to the basal lamina,[4] They also have non-motile stereocilia, which are long and branching in the head region and shorter in the tail region.[4] They also secrete carnitine, sialic acid, glycoproteins, and glycerylphosphorylcholine into the lumen.
  • Basal cells: shorter, pyramid-shaped cells which contact the basal lamina but taper off before their apical surfaces reach the lumen.[4] These are thought to be undifferentiated precursors of principal cells.[4]
  • Apical cells: predominantly found in the head region[4]
  • Clear cells: predominant in the tail region[4]
  • Intraepithelial lymphocytes: distributed throughout the tissue.[4]
  • Intraepithelial macrophages[5][6]


The stereocilia of the epididymis are long cytoplasmic projections that have no motility and which aid in absorption.

These numerous apical modifications are often referred to as "stereocilia", as under the light microscope they look like cilia or the stereocilia of the inner ear. However, as electron microscopy has revealed them to be structurally and functionally more similar to the long, absorptive microvilli of other epithelia, some now refer to them as stereovilli.[7] These membrane extensions increase the surface area of the cell, allowing for greater absorption and secretion.[8]

The stereocilia in the epididymis are shaped by an internal actin network with no microtubule structure, and unlike true cilia are non-motile.[9] Because sperm are initially non-motile as they leave the seminiferous tubules, large volumes of fluid are secreted to propel them to the epididymis. The core function of the stereocilia is to resorb 90% of this fluid as the spermatozoa start to become motile. This absorption creates a fluid current that moves the immobile sperm from the seminiferous tubules to the epididymis. Spermatozoa do not reach full motility until they reach the vagina, where the alkaline pH is neutralized by acidic vaginal fluids.


In the embryo, the epididymis develops from tissue that once formed the mesonephros, a primitive kidney found in many aquatic vertebrates. Persistence of the cranial end of the mesonephric duct will leave behind a remnant called the appendix of the epididymis. In addition, some mesonephric tubules can persist as the paradidymis, a small body caudal to the efferent ductules.

A Gartner's duct is a homologous remnant in the female.


Role in storage of sperm and ejaculant

Spermatozoa formed in the testis enter the caput epididymis, progress to the corpus, and finally reach the cauda region, where they are stored. Sperm entering the caput epididymis are incomplete—they lack the ability to swim forward (motility) and to fertilize an egg. It stores the sperm for 2–3 months. During their transit in the epididymis, sperm undergo maturation processes necessary for them to acquire these functions.[10] Final maturation is completed in the female reproductive tract (capacitation).

The epididymis secretes some proteins that blocks the receptors on the plasma membrane of sperm head which renders sperm infertile inside the male tract (decapacitation).

During ejaculation, sperm flow from the lower portion of the epididymis (which functions as a storage reservoir). They have not been activated by products from the prostate gland, and they are unable to swim, but are transported via the peristaltic action of muscle layers within the vas deferens, and are mixed with the diluting fluids of the seminal vesicles and other accessory glands prior to ejaculation (forming semen).

Clinical significance


An inflammation of the epididymis is called epididymitis. It is much more common than testicular inflammation, termed orchitis.

Surgical removal

Epididymotomy is the placing of an incision into the epididymis and is sometimes considered as a treatment option for acute suppurating epididymitis. Epididymectomy is the surgical removal of the epididymis sometimes performed for post-vasectomy pain syndrome and for refractory cases of epididymitis.

Popular culture

Ghostbusters II

In the 1989 film, Egon Spengler (played by the late Harold Ramis) responds to a jibe made by Peter Venkman (Bill Murray) about his female colleagues being attracted to the size of his cranium with "I think they're more interested in my epididymis."[11]


See also


  1. ^ Kim, Howard H.; Goldstein, Marc (2010). "Chapter 53: Anatomy of the epididymis, vas deferens, and seminal vesicle". In Graham, Sam D.; Keane, Thomas E.; Glenn, James F. Glenn's urological surgery (7th ed.). Philadelphia: Lippincott Williams & Wilkins. p. 356. ISBN 978-0-7817-9141-0. 
  2. ^ a b c Bacha, William; Bacha, Linda (2012). Color Atlas of Veterinary Histology. Wiley-Blackwell. p. 226. ISBN 0470958510. 
  3. ^ Romer, Alfred Sherwood; Parsons, Thomas S. (1977). The Vertebrate Body. Philadelphia, PA: Holt-Saunders International. pp. 394–395. ISBN 0-03-910284-X. 
  4. ^ a b c d e f g Kierszenbaum, Abraham L. (2002). Histology and cell biology : an introduction to pathology. St. Louis: Mosby. p. 556. ISBN 0-323-01639-1. 
  5. ^ Da Silva N, Cortez-Retamozo V, Reinecker HC, et al. (May 2011). "A dense network of dendritic cells populates the murine epididymis". Reproduction. 141 (5): 653–63. doi:10.1530/REP-10-0493. PMC 3657760Freely accessible. PMID 21310816. 
  6. ^ Shum WW, Smith TB, Cortez-Retamozo V, et al. (May 2014). "Epithelial basal cells are distinct from dendritic cells and macrophages in the mouse epididymis". Biology of Reproduction. 90 (5): 90. doi:10.1095/biolreprod.113.116681. PMC 4076373Freely accessible. PMID 24648397. 
  7. ^ Ross, Michael H.; Pawlina, Wojciech (2011). Histology: A Text and Atlas. Lippincott Williams & Wilkins. pp. 110–112. ISBN 978-0-7817-7200-6. 
  8. ^ How sperm are re-absorbed into the body. vasectomy-information.com
  9. ^ Efferent Ducts and Epididymis. umdnj.edu[unreliable medical source?]
  10. ^ Jones RC (April 1999). "To store or mature spermatozoa? The primary role of the epididymis". International Journal of Andrology. 22 (2): 57–67. doi:10.1046/j.1365-2605.1999.00151.x. PMID 10194636. 
  11. ^ http://www.imdb.com/title/tt0097428/quotes?item=qt0407325

External links