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Electroantennography or EAG is a technique for measuring the average output of an insect
The technique is widely applied in screening of insect pheromones by examining the responses to fractions of a compound mixture separated using chromatography.Hummel, H. E. and Miller, T. A. (1984). ''Techniques in pheromone research''. Springer-Verlag, New York. Usually, the wire inserted into the antenna is a thin silver wire that is chlorided in bleach. This is an older practice. Commonly, tungsten wires that have been chemically sharpened are inserted into a single neuron in the antenna. Further detailed examination of the odor response at the olfactory sensory level can be done by
Aphid Olfaction
Walter Leal Lab Home Page
Animal anatomy
antenna
Antenna ( antennas or antennae) may refer to:
Science and engineering
* Antenna (radio), also known as an aerial, a transducer designed to transmit or receive electromagnetic (e.g., TV or radio) waves
* Antennae Galaxies, the name of two collid ...
to its brain for a given odor
An odor (American English) or odour (English in the Commonwealth of Nations, Commonwealth English; American and British English spelling differences#-our, -or, see spelling differences) is caused by one or more volatilized chemical compounds ...
. It is commonly used in electrophysiology while studying the function of the olfactory
The sense of smell, or olfaction, is the special sense through which smells (or odors) are perceived. The sense of smell has many functions, including detecting desirable foods, hazards, and pheromones, and plays a role in taste.
In humans, it ...
pathway in insects. The technique was invented in 1957 by German biologist Dietrich Schneider and shares similarities with electro-olfactography
Electro-olfactography or electroolfactography (EOG) is a type of electrography (electrophysiologic test) that aids the study of olfaction (the sense of smell). It measures and records the changing electrical potentials of the olfactory epithelium ...
.{{Citation , editor-last=Glatz , editor-first=Richard , year=2015 , title=Molecular Basis of Olfaction. Volume 130 of Progress in Molecular Biology and Translational Science , publisher=Academic Press, page=ix , url=https://books.google.com/books?id=LFOZBQAAQBAJ&pg=PR9 , isbn=978-0128029138 , postscript=.
Method
Electroantennography is usually performed either by removing an antenna from the animal and inserting two chlorided silver wires for contact onto the two ends and amplifying the voltage between them while applying an odor puff to see a deflection as in the figure, or by leaving the animal intact and inserting a ground wire ( silver/ silver chloride) or a glass electrode filled with a buffer solution to some part of the body, usually inserted into an eye, and another to the tip of the antenna. A large bore glass electrode can also be placed directly over the tip of the antenna, such as in '' Drosophila melanogaster'' (fruit fly) antenna recordings. The latter method is useful if one is doing an experiment on the animal as a whole while doing the antennogram.
The technique is widely applied in screening of insect pheromones by examining the responses to fractions of a compound mixture separated using chromatography.Hummel, H. E. and Miller, T. A. (1984). ''Techniques in pheromone research''. Springer-Verlag, New York. Usually, the wire inserted into the antenna is a thin silver wire that is chlorided in bleach. This is an older practice. Commonly, tungsten wires that have been chemically sharpened are inserted into a single neuron in the antenna. Further detailed examination of the odor response at the olfactory sensory level can be done by sensilla
A sensillum (plural ''sensilla'') is an arthropod sensory organ protruding from the cuticle of exoskeleton, or sometimes lying within or beneath it. Sensilla appear as small hairs or pegs over an individual's body. Inside each sensillum there are ...
recording.
References
External links
Aphid Olfaction
Walter Leal Lab Home Page
Animal anatomy