Dichlorofluorescein (DCF) is an organic dye of the

fluorescein Fluorescein is an organic compound and dye based on the xanthene tricyclic structural motif, formally belonging to triarylmethine dyes family. It is available as a dark orange/red powder slightly soluble in water and alcohol. It is widely used ...

family, being substituted at the 2 and 7 positions by chloride. It is used as an indicator for argentometry by Fajans method. When used as an indicator, upon reaching the equivalence point of a titration reaction the color shifts from colorless towards a faint pink. It is also used in the

cellular antioxidant activity Cellular may refer to: *Cellular automaton, a model in discrete mathematics *Cell biology, the evaluation of cells work and more * ''Cellular'' (film), a 2004 movie *Cellular frequencies, assigned to networks operating in cellular RF bands *Cellu ...

(CAA) assay. Dichlorofluorescin (DCFH) is a probe that is trapped within cells and is easily oxidized to fluorescent dichlorofluorescein (DCF). The method measures the ability of compounds to prevent the formation of DCF by 2,2'-Azobis(2-amidinopropane) dihydrochloride (ABAP)-generated peroxyl radicals in human hepatocarcinoma HepG2 cells. By itself, dichlorofluorescin (DCFH) also quantifies intracellular hydrogen peroxide as well as cellular

oxidative stress Oxidative stress reflects an imbalance between the systemic manifestation of reactive oxygen species and a biological system's ability to readily Detoxification, detoxify the reactive intermediates or to repair the resulting damage. Disturbances ...

References

{{Reflist Fluorone dyes Chloroarenes Spiro compounds Hydroxyarenes Triarylmethane dyes