Plasmodium glutamate dehydrogenase precipitation

Counterimmunoelectrophoresis is a

laboratory technique A laboratory (; ; colloquially lab) is a facility that provides controlled conditions in which scientific or technological research, experiments, and measurement may be performed. Laboratory services are provided in a variety of settings: physici ...

used to evaluate the binding of an

antibody An antibody (Ab), also known as an immunoglobulin (Ig), is a large, Y-shaped protein used by the immune system to identify and neutralize foreign objects such as pathogenic bacteria and viruses. The antibody recognizes a unique molecule of the ...

to its

antigen In immunology, an antigen (Ag) is a molecule or molecular structure or any foreign particulate matter or a pollen grain that can bind to a specific antibody or T-cell receptor. The presence of antigens in the body may trigger an immune respons ...

, it is similar to immunodiffusion, but with the addition of an applied

electrical field An electric field (sometimes E-field) is the physical field that surrounds electrically charged particles and exerts force on all other charged particles in the field, either attracting or repelling them. It also refers to the physical field f ...

across the diffusion medium, usually an agar or

polyacrylamide Polyacrylamide (abbreviated as PAM) is a polymer with the formula (-CH2CHCONH2-). It has a linear-chain structure. PAM is highly water-absorbent, forming a soft gel when hydrated. In 2008, an estimated 750,000,000 kg were produced, mainly f ...

gel. The effect is rapid migration of the antibody and antigen out of their respective wells towards one another to form a line of precipitation, or a

precipitin A precipitin is an antibody which can precipitate out of a solution upon antigen binding. Precipitin reaction The precipitin reaction provided the first quantitative assay for antibody. The precipitin reaction is based upon the interaction of an ...

line, indicating binding.

References

External links

* * https://web.archive.org/web/20070613005107/http://www.lib.mcg.edu/edu/esimmuno/ch4/electro.htm Immunologic tests Blood tests {{immunology-stub

See also

References

External links