Chromatography column
   HOME

TheInfoList



OR:

A Chromatography column is a device used in
chromatography In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the ''mobile phase'', which carries it through a system (a ...
for the separation of chemical compounds. A chromatography column contains the stationary phase, allowing the
mobile phase In analytical and organic chemistry, elution is the process of extracting one material from another by washing with a solvent; as in washing of loaded ion-exchange resins to remove captured ions. In a liquid chromatography experiment, for exa ...
to pass through it. Chromatography columns of different types are used in both
gas Gas is one of the four fundamental states of matter (the others being solid, liquid, and plasma). A pure gas may be made up of individual atoms (e.g. a noble gas like neon), elemental molecules made from one type of atom (e.g. oxygen), or ...
and
liquid chromatography In chemical analysis, chromatography is a laboratory technique for the Separation process, separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the ''mobile phase'', which carries it ...
.


Materials

* Liquid chromatography: Traditional chromatography columns were made of glass. Modern columns are mostly made of
borosilicate glass Borosilicate glass is a type of glass with silica and boron trioxide as the main glass-forming constituents. Borosilicate glasses are known for having very low coefficients of thermal expansion (≈3 × 10−6 K−1 at 20 °C), ma ...
,
acrylic glass Poly(methyl methacrylate) (PMMA) belongs to a group of materials called engineering plastics. It is a transparent thermoplastic. PMMA is also known as acrylic, acrylic glass, as well as by the trade names and brands Crylux, Plexiglas, Acrylite, ...
or
stainless steel Stainless steel is an alloy of iron that is resistant to rusting and corrosion. It contains at least 11% chromium and may contain elements such as carbon, other nonmetals and metals to obtain other desired properties. Stainless steel's corros ...
. To prevent the stationary phase from leaking out of the column interior a polymer, stainless steel or ceramic net is usually applied. Depending on the application material- and size-requirements may change. * Gas chromatography (GC): Older columns were made of glass or metal packed with particles of a solid stationary phase. More recently, narrower diameter (capillary) columns have been made using fused silica coated on the inside with a film of the stationary phase material. GC columns are typically very long to take advantage of their low resistance to the flow of carrier gas. The materials of the column and the stationary phase must be suitable for GC operating temperatures, which may range as high as 300°C or more.


Sizes

While small-scale columns range from inner
diameters In geometry, a diameter of a circle is any straight line segment that passes through the center of the circle and whose endpoints lie on the circle. It can also be defined as the longest chord of the circle. Both definitions are also valid fo ...
of 0.5 cm and withstand pressures of up to 130
MPa MPA or mPa may refer to: Academia Academic degrees * Master of Performing Arts * Master of Professional Accountancy * Master of Public Administration * Master of Public Affairs Schools * Mesa Preparatory Academy * Morgan Park Academy * Mound ...
,UHPLC in Life Sciences, Davy Guillarme, Jean-Luc Veuthey, Roger M. Smith, Royal Society of Chemistry, 2012 industrial large scale columns reach diameters of up to 2 m and operate at considerable lower pressures (below 1 MPa). While it is favorable to view the packed bed of a column large scale columns are manufactured from steel due to its superior resilience. Chromatography columns can be used as stand-alone devices or in combination with manual or automated chromatography systems. Medium to large columns are almost exclusively operated together with automated systems to decrease the risk of process failure and loss of product.


Different columns for different scales


Small scale

Transitions between scales are always fluent. There is no sharp cut that defines the end of small- and the beginning of medium/pilot scale. However, chromatography columns with an inner diameter (ID) of up to 5 cm are generally considered small scale or laboratory scale columns. Small scale chromatography columns are mostly intended for
design of experiments The design of experiments (DOE, DOX, or experimental design) is the design of any task that aims to describe and explain the variation of information under conditions that are hypothesized to reflect the variation. The term is generally associ ...
(DoE);
proof of concept Proof of concept (POC or PoC), also known as proof of principle, is a realization of a certain method or idea in order to demonstrate its feasibility, or a demonstration in principle with the aim of verifying that some concept or theory has prac ...
;
validation (drug manufacture) Validation is the process of establishing documentary evidence demonstrating that a procedure, process, or activity carried out in testing and then production maintains the desired level of compliance at all stages. In the pharmaceutical industry, ...
or
research and development Research and development (R&D or R+D), known in Europe as research and technological development (RTD), is the set of innovative activities undertaken by corporations or governments in developing new services or products, and improving existi ...
experiments. Columns of this scale category are distinguished by their small dimensions in comparison to chromatography columns intended for larger scales as well as relatively high pressure tolerance and selection of materials in contact with the liquid phase. This is especially important for applications in the
biopharmaceutical A biopharmaceutical, also known as a biological medical product, or biologic, is any pharmaceutical drug product manufactured in, extracted from, or semisynthesized from biological sources. Different from totally synthesized pharmaceuticals, t ...
industry which underlie close scrutiny by regulatory agencies (U.S.
Food and Drug Administration The United States Food and Drug Administration (FDA or US FDA) is a List of United States federal agencies, federal agency of the United States Department of Health and Human Services, Department of Health and Human Services. The FDA is respon ...
;
European Medicines Agency The European Medicines Agency (EMA) is an agency of the European Union (EU) in charge of the evaluation and supervision of medicinal products. Prior to 2004, it was known as the European Agency for the Evaluation of Medicinal Products or Euro ...
).


See also

*
Separation process A separation process is a method that converts a mixture or a solution (chemistry), solution of chemical substances into two or more distinct product mixtures, a scientific process of separating two or more substance in order to obtain purity. At ...


References


External links

*{{Commonscatinline, Column chromatography
Learn More About Chromatography Columns
Chromatography