Brachyspira

''Brachyspira'' is a genus of bacteria classified within the phylum Spirochaetota.See the List of Prokaryotic names with Standing in Nomenclature. Data extracted from
See the NCBIbr>webpage on Spirochaetes
Data extracted from

''Brachyspira species'' include pathogens in pigs, birds, dogs, and humans.

'' B. pilosicoli'' colonizes millions of humans worldwide, leading to human intestinal spirochaetosis, a chronic, intermittent watery diarrhea vastly underdiagnosed because of the lack of a simple diagnostic tool for clinicians. Multiplex qPCRs are promising diagnostic tools, as Brachyspira do not grow on conventional media.

'' B. pilosicoli'' also cause avian spirochetosis: birds might be considered as the natural reservoir.

'' B. hyodysenteriae'' leads to diarrheal disease in growing pigs worldwide, causing the so-called swine dysentery, typhlocolitis or porcine intestinal spirochaetosis, which contributes to major "production losses" in agrobusiness.

Some species like '' B. innocens'' or '' B. intermedia'' seem to be less virulent.

Phylogeny

The currently accepted taxonomy is based on the List of Prokaryotic names with Standing in Nomenclature (LPSN) and National Center for Biotechnology Information (NCBI).

Evolutionary hypothesis

It is interesting to consider that Brachyspira could be the missing link between independent gram-negatives and eventually internalized organisms like Mitochondria. One could imagine the following phylogenetic pathway: gram-negative free dwellers -> spirochetes attached to cell cytoskeleton and expressing porins creating cytoplasmic bridges and genome complementarity between parasite and mother cell -> rickettsia with full internalization --> permanent intracellular host = mitochondrion

Pathogenesis of human intestinal spirochetosis (HIS)

Brachyspira bacteria have evolved a parasitic lifestyle through genomic reduction (~2.5 to 3.3 Mb) compared to other gram negative bacteria (~5 Mb).

Humans become infected through dirty water ingestion, possibly by swimming in waters containing the bacteria or by direct oral exposure to contaminated feces (outdoor tribes, raw egg eaters, slum inhabitants with no sanitation, MSM).

Genome homologies between Borellia, Treponema and Brachyspira imply that Brachyspira is expected to:
* import carbohydrates and short fatty acids (6->3 carbons) for its energetic needs from the colon lumen,
* swim to (viscophily) and through (viscotaxy) mucin layers thanks to its spiroid shape and flagellum (see film ),
* attach to colonocytes apically and to each other laterally,
* thereby creating a continuous layer of bacterial cells which can withstand feces movement in vivo: this is the pathognomonic brush borderbr>
seen in histology on colonic biopsies
*it is still to be elucidated if Brachyspira is, as Borrelia, able to attach to decorin and progress in loose connective tissue and invade other tissues,
*as for borrelia and syphilis, Brachyspira may be able to translocate to seminal vesicles where it would find another niche "outside" the body with mucins to invade, epithelia to attach and glucose available. Brachyspira may be a sexually transmittable disease in MSM communities via ano-oral route but also penetrative route.

Once attached apically to the enterocyte, hidden to the natural and acquired immunity by the mucous layer and occupying a niche that other bacteria cannot use, Brachyspira most likely expresses at its apex porin

allowing it to import from the colonocyte's cytoplasm the amino acids and nucleic acids necessary to replicate.

It has also been demonstrated that Brachyspira creates an environment which is favorable to its locomotion by upregulating mucin expression: it creates its own niche.

Clinical manifestations in human medicine

Publications now tend to point out that Brachyspira colonization should ''not'' be considered harmless commensalism:
* Chronic diarrhea
* Irritable bowel syndrome
* Acute intestinal pain
* Ulcerative colitis
* Post translocation spirochetemia and cardiogenic shock

Antibiotic treatment and resistances in human medicine

Treament with 10 days co-amoxicilline 1g bid + metronidazole 500 tid seems to have very good results on abdominal symptoms. It is advised to administer '' Saccharomyces boulardii'' once a day during this course of antibiotherapy.

Doxycycline resistance has been documented and should be avoided.

Antibiotic treatment and resistance in veterinary medicine

Veterinary antibiotics used to treat pigs with dysentery due to ''Brachyspira species'' include the lincosamide lincomycin, the ionophore salinomycin, the quinoxaline carbadox, the pleuromodulins tiamulin and valnemulin, as well as the aminoglycoside gentamicin, an important antibiotic used in humans.

''Brachyspira'' resistance to the above antibiotics has been increasingly reported. While no Clinical and Laboratory Standards Institute (CLSI) antimicrobial breakpoints for ''Brachyspira'' have been established, resistance to the pleuromodulins tiamulin and valnemulin is considered at MIC ≥ 2 µg/ml. Resistance to pleuromodulins is important, because they are antibiotics of "last resort"; as of 2001, they were the only antibiotics with sufficient minimum inhibitory concentration (MIC) values left to treat swine dysentery in Sweden, per the National Veterinary Institute in Uppsala.

Antibiotic resistance varies by geographic region and is not developing as rapidly in U.S. isolates as has been seen in isolates from other countries.Clothier KA, Kinyon JM, Frana TS, Naberhaus N, Bower L, Strait EL, Schwartz K.Species characterization and minimum inhibitory concentration patterns of Brachyspira species isolates from swine with clinical disease.J Vet Diagn Invest. 2011 Nov;23(6):1140-5.
Tiamulin resistance was first described in 1996 in Hungary, and subsequently reported from other countries in Europe and Asia,.
In Spain, 7.4% of Brachyspira isolates were reported to be venamulin-resistant and 17.6% were tiamulin-resistant in 2009. In Sweden, 10-15% of ''B. pilosicoli'' isolates between 2002 and 2010 were resistant to tiamulin (MICs >4 μg/ml), and a gradual increase in tiamulin MICs was seen in ''B. hyodysenteriae'' between 1990 and 2003, which has since plateaued.

Decreased susceptibility to lincomycin, but not to tiamulin was found among Polish isolates.

In the US, resistance of Brachyspira species collected 2008–2010 was common only against lincomycin (80% had MIC of 32 or 64), MIC's were moderately high against gentamicin, while resistance to valnemulin(4.7%) and tiamulin (3.2% of isolates) was yet uncommon, as reported in the only U.S. study to date, from Iowa.

The use of pleuromodulins in U.S. food animals is not separately reported in the U.S. Food and Drug Administration's annual Animal Drug User Fee Act (ADUFA) report, "Antimicrobials Sold or Distributed for Use in Food-Producing Animals". However, the amount of 190 tonnes of lincosamides used is substantial per ADUFA; antibiotics used in the U.S. in food animals in 2011 was: Ionophores 4,123,259 kg, aminoglycosides 214,895 kg, and Lincosamides 190,101 kg.

Microbiologic identification

Brachyspira are capable of hemolysis, the degree of which has been used to characterize them, with ''B. hyodysenteriae'' showing strong beta hemolysis while ''B. pilosicoli, B. intermedia, B. murdochii,'' and ''B. innocens'' have been described as weakly hemolytic. However, in a recent study from Iowa State University, all (10/10) ''B. intermedia'' isolates, 91% (9/11) of ''Brachyspira spp.'' isolates, and 20% (2/6) of ''B. pilosicoli'' isolates from farms in North Carolina (36), Iowa (23), Minnesota (9), Nebraska (3), Michigan (2), Illinois (2), Missouri (1), North Dakota (1), South Dakota (1), and Ohio (1), demonstrated strong beta-hemolysis.

Recently quantitative PCR seems to be a more sensitive way to identify Brachyspira, which is globally a very fastidious bacterium to grow.

Change in ecology

In the U.S.A. Brachyspira-associated pig disease and isolation of ''Brachyspira species'' from swine with diarrheal disease largely disappeared from swine herds in the late 1990s and early 2000s, but returned in the mid-2000s for unknown reasons.

A 2011 study of isolates from Midwestern swine herds described major changes in Brachyspira spp frequency and hemolysis, i.e. pathogenicity: the majority of isolated Brachyspira species were previously considered minimally pathogenic or commensal, like ''Brachyspira murdochi'' (27%)or novel/unclassifiable Brachyspira species (25%), while only 40.5% of 79 isolates from diseased pigs could be confirmed as the classic pathogens ''B. hyodysenteriae'' or ''Brachyspira pilosicoli'' by PCR.Clothier KA, Kinyon JM, Frana TS, Naberhaus N, Bower L, Strait EL, Schwartz K.Species characterization and minimum inhibitory concentration patterns of ''Brachyspira'' species isolates from swine with clinical disease.J Vet Diagn Invest. 2011 Nov;23(6):1140-5.
Brachyspira species previously capable of weak hemolysis only, like ''B. intermedia'' and ''B. pilosicoli'' were found to produce strong hemolysis. They were also frequently identified from diseased swine which suggests they are emerging pathogens.

A compelling explanation for this change in epidemiology and ecology is selection by the increasing use of antibiotics in pigs (e.g. as growth promoters), since B. murdochii and unclassifiable Brachyspira spp. are less susceptible to antimicrobials than the previously established Brachyspira pathogens.

See also

* List of bacteria genera
* List of bacterial orders

References

{{Taxonbar, from=Q896648

Spirochaetes
Gram-negative bacteria