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BioPlex (biophysical interactions of ORFeome-based complexes) is an open access resource for studying
BioPlex (biophysical interactions of ORFeome-based complexes) is an open access resource for studying protein
Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues. Proteins perform a vast array of functions within organisms, including catalysing metabolic reactions, DNA replication, res ...
-protein interactions. It is the result of collaborations between Harvard Medical School
Harvard Medical School (HMS) is the graduate medical school of Harvard University and is located in the Longwood Medical Area of Boston, Massachusetts. Founded in 1782, HMS is one of the oldest medical schools in the United States and is consi ...
and Biogen
Biogen Inc. is an American multinational biotechnology company based in Cambridge, Massachusetts, specializing in the discovery, development, and delivery of therapies for the treatment of neurological diseases to patients worldwide.
History
...
. BioPlex 1.0 reported 23,744 interactions among 7,668 proteins. BioPlex 2.0 extended those observations to detect over 29,000 new interactions.
The basic technology is to express a "bait" protein in human cells. Those bait proteins interact with other proteins, and then the complexes of the bait and "prey" proteins are isolated by affinity purification
Affinity chromatography is a method of separating a biomolecule from a mixture, based on a highly specific macromolecular binding interaction between the biomolecule and another substance. The specific type of binding interaction depends on the ...
. The interacting prey proteins are identified using mass spectrometry.
Use in research
Determining the interaction partners of poorly characterized proteins can provide clues to the function of those proteins, and knowing which "protein community" a disease gene resides in can give better context for its action.See also
* Human proteome project *Interactome In molecular biology, an interactome is the whole set of molecular interactions in a particular cell. The term specifically refers to physical interactions among molecules (such as those among proteins, also known as protein–protein interactions, ...
*Proteome
The proteome is the entire set of proteins that is, or can be, expressed by a genome, cell, tissue, or organism at a certain time. It is the set of expressed proteins in a given type of cell or organism, at a given time, under defined conditions. ...
References
{{reflist Proteomics Biological databases