American Conference may refer to:
*American Conference of Governmental Industrial Hygienists, professional organization in the field of industrial hygiene
*American Unitarian Conference, a religious organization founded in 2000 by Unitarian Universalists
*Pan-American Conference, an international trade summit held in Bogotá, Colombia in 1948
*In school sports:
**American Conference (CIF), a California high-school organization
**American Athletic Conference, an NCAA Division I conference reorganized in 2013 from the former Big East Conference
*In professional American football:
**American Football Conference (AFC) of the National Football League
**American Conference of the Arena Football League (1987–2008), Arena Football League
**All-America Football Conference, a league which played from 1946 to 1949
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