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Deinococcus-Thermus
''Deinococcota'' (synonym, ''Deinococcus-Thermus'') is a phylum of bacteria with a single class, ''Deinococci'', that are highly resistant to environmental hazards, also known as extremophiles. These bacteria have thick cell walls that give them gram-positive stains, but they include a second membrane and so are closer in structure to those of gram-negative bacteria. Taxonomy The phylum ''Deinococcota'' consists of a single class ('' Deinococci'') and two orders: * The '' Deinococcales'' include two families ('' Deinococcaceae'' and'' Trueperaceae''), with three genera, '' Deinococcus'', '' Deinobacterium'' and '' Truepera''.Garrity GM, Holt JG. (2001) Phylum BIV. "Deinococcus–Thermus". In: Bergey’s manual of systematic bacteriology, pp. 395-420. Eds D. R. Boone, R. W. Castenholz. Springer-: New York.Garrity GM, Bell JA, Lilburn TG. (2005) Phylum BIV. The revised road map to the Manual. In: Bergey’s manual of systematic bacteriology, pp. 159-220. Eds Brenner DJ, Krieg NR, ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Deinococcales
''Deinococcota'' (synonym, ''Deinococcus-Thermus'') is a phylum of bacteria with a single class, ''Deinococci'', that are highly resistant to environmental hazards, also known as extremophiles. These bacteria have thick cell walls that give them gram-positive stains, but they include a second membrane and so are closer in structure to those of gram-negative bacteria. Taxonomy The phylum ''Deinococcota'' consists of a single class ('' Deinococci'') and two orders: * The '' Deinococcales'' include two families ('' Deinococcaceae'' and'' Trueperaceae''), with three genera, '' Deinococcus'', '' Deinobacterium'' and '' Truepera''.Garrity GM, Holt JG. (2001) Phylum BIV. "Deinococcus–Thermus". In: Bergey’s manual of systematic bacteriology, pp. 395-420. Eds D. R. Boone, R. W. Castenholz. Springer-: New York.Garrity GM, Bell JA, Lilburn TG. (2005) Phylum BIV. The revised road map to the Manual. In: Bergey’s manual of systematic bacteriology, pp. 159-220. Eds Brenner DJ, Krieg NR, ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Deinococci
''Deinococcota'' (synonym, ''Deinococcus-Thermus'') is a phylum of bacteria with a single class, ''Deinococci'', that are highly resistant to environmental hazards, also known as extremophiles. These bacteria have thick cell walls that give them gram-positive stains, but they include a second membrane and so are closer in structure to those of gram-negative bacteria. Taxonomy The phylum ''Deinococcota'' consists of a single class ('' Deinococci'') and two orders: * The ''Deinococcales'' include two families ('' Deinococcaceae'' and'' Trueperaceae''), with three genera, '' Deinococcus'', '' Deinobacterium'' and '' Truepera''.Garrity GM, Holt JG. (2001) Phylum BIV. "Deinococcus–Thermus". In: Bergey’s manual of systematic bacteriology, pp. 395-420. Eds D. R. Boone, R. W. Castenholz. Springer-: New York.Garrity GM, Bell JA, Lilburn TG. (2005) Phylum BIV. The revised road map to the Manual. In: Bergey’s manual of systematic bacteriology, pp. 159-220. Eds Brenner DJ, Krieg NR, S ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Thermales
''Thermales'' is an order of bacteria belonging to the ''Deinococcota'' phylum. They are particularly resistant to heat, and live in the benthic zone of the Gulf of Mexico. Members of the order ''Thermales'' can be distinguished from all other bacteria through molecular signatures consisting of conserved signature indels (CSIs) and conserved signature proteins (CSPs) that are exclusively present in members of this order. Specifically, six CSIs were identified in the following proteins: DNA topoisomerase I, ABC transporter permease, citrate synthase, phosphoribosylformylglycinamidine synthase, pyruvate dehydrogenase and alpha-glucan phosphorylase. 51 CSPs were also found to be exclusively shared by members of this order. In addition, a 76 aa CSI is present in the protein SecA preprotein translocase which is specific to all members of the order ''Thermales'' as well as ''Hydrogenibacillus schlegelii'' (also an thermophilic species). Based on studies on other CSIs and CSPs, it is ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Thermaceae
''Thermales'' is an order of bacteria belonging to the ''Deinococcota'' phylum. They are particularly resistant to heat, and live in the benthic zone of the Gulf of Mexico. Members of the order ''Thermales'' can be distinguished from all other bacteria through molecular signatures consisting of conserved signature indels (CSIs) and conserved signature proteins (CSPs) that are exclusively present in members of this order. Specifically, six CSIs were identified in the following proteins: DNA topoisomerase I, ABC transporter permease, citrate synthase, phosphoribosylformylglycinamidine synthase, pyruvate dehydrogenase and alpha-glucan phosphorylase. 51 CSPs were also found to be exclusively shared by members of this order. In addition, a 76 aa CSI is present in the protein SecA preprotein translocase which is specific to all members of the order ''Thermales'' as well as ''Hydrogenibacillus schlegelii'' (also an thermophilic species). Based on studies on other CSIs and CSPs, it is ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Meiothermus
''Meiothermus'' is a genus of ''Deinococcota'' bacteria. Members of ''Meiothermus'' can be reliably distinguished from other genera in the family '' Thermaceae'' as well as all other bacteria by the presence of three conserved signature indels (CSIs) found in the proteins: 5-methyltetrahydrofolate–homocysteine methyltransferase, cadmium transporter and polynucleotide phosphorylase and are exclusively shared by species of this genus. ''Meiothermus'' is also different than the '' Thermus'' genus, which it was originally a member of, in their optimum growth temperatures, with ''Meiothermus'' being able to grow in colder environments. ''Meiothermus'' was first isolated with ''Thermus'' in alkaline and neutral hot springs in Kamchatka, Russia and Yellowstone National Park, USA. Characteristics ''Meiothermus'' species of bacteria are gram-negative and are primarily chemoorganoheterotrophs, but some species can use nitrate as an electron acceptor. The ''Meiothermus'' genus wa ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Thermus
''Thermus'' is a genus of thermophilic bacteria. It is one of several bacteria belonging to the ''Deinococcota'' phylum. ''Thermus'' species can be distinguished from other genera in the family ''Thermaceae'' as well as all other bacteria by the presence of eight conserved signature indels (CSIs) found in proteins such as adenylate kinase and replicative DNA helicase as well as 14 conserved signature proteins (CSPs) that are exclusively shared by members of this genus. Phylogeny The currently accepted taxonomy is based on the List of Prokaryotic names with Standing in Nomenclature (LPSN) and the National Center for Biotechnology Information (NCBI). Unassigned species: * "'' T. anatoliensis''" Kacagan et al. 2016 * "'' T. caldophilus''" Taguchi et al. 1983 * "'' T. eggertssonii''" Peters 2008 * "'' T. murrieta''" Benner et al. 2006 * "'' T. nonproteolyticus''" 1992 * "'' T. rehai''" Lin et al. 2002 * "'' T. yunnanensis''" Gong et al. 2005 See also * Bacteria * Biotechnol ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Thermus Aquaticus
''Thermus aquaticus'' is a species of bacteria that can tolerate high temperatures, one of several thermophilic bacteria that belong to the ''Deinococcota'' phylum. It is the source of the heat-resistant enzyme ''Taq'' DNA polymerase, one of the most important enzymes in molecular biology because of its use in the polymerase chain reaction (PCR) DNA amplification technique. History When studies of biological organisms in hot springs began in the 1960s, scientists thought that the life of thermophilic bacteria could not be sustained in temperatures above about . Soon, however, it was discovered that many bacteria in different springs not only survived, but also thrived in higher temperatures. In 1969, Thomas D. Brock and Hudson Freeze of Indiana University reported a new species of thermophilic bacteria which they named ''Thermus aquaticus''. The bacterium was first isolated from Mushroom Spring in the Lower Geyser Basin of Yellowstone National Park, which is near the major ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Extremophilic
An extremophile (from Latin ' meaning "extreme" and Greek ' () meaning "love") is an organism that is able to live (or in some cases thrive) in extreme environments, i.e. environments that make survival challenging such as due to extreme temperature, radiation, salinity, or pH level. These organisms are ecologically dominant in the evolutionary history of the planet. Some spores and cocooned bacteria samples have been dormant for more than 40 million years, extremophiles have continued to thrive in the most extreme conditions, making them one of the most abundant lifeforms. Characteristics In the 1980s and 1990s, biologists found that microbial life has great flexibility for surviving in extreme environments—niches that are acidic, extraordinarily hot or within irregular air pressure for example—that would be completely inhospitable to complex organisms. Some scientists even concluded that life may have begun on Earth in hydrothermal vents far under the ocean's surfac ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Polymerase Chain Reaction
The polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies (complete or partial) of a specific DNA sample, allowing scientists to take a very small sample of DNA and amplify it (or a part of it) to a large enough amount to study in detail. PCR was invented in 1983 by the American biochemist Kary Mullis at Cetus Corporation; Mullis and biochemist Michael Smith, who had developed other essential ways of manipulating DNA, were jointly awarded the Nobel Prize in Chemistry in 1993. PCR is fundamental to many of the procedures used in genetic testing and research, including analysis of ancient samples of DNA and identification of infectious agents. Using PCR, copies of very small amounts of DNA sequences are exponentially amplified in a series of cycles of temperature changes. PCR is now a common and often indispensable technique used in medical laboratory research for a broad variety of applications including biomedical research ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Conserved Signature Indels
Conserved signature inserts and deletions (CSIs) in protein sequences provide an important category of molecular markers for understanding phylogenetic relationships. CSIs, brought about by rare genetic changes, provide useful phylogenetic markers that are generally of defined size and they are flanked on both sides by conserved regions to ensure their reliability. While indels can be arbitrary inserts or deletions, CSIs are defined as only those protein indels that are present within conserved regions of the protein. The CSIs that are restricted to a particular clade or group of species, generally provide good phylogenetic markers of common evolutionary descent. Due to the rarity and highly specific nature of such changes, it is less likely that they could arise independently by either convergent or parallel evolution (i.e. homoplasy) and therefore are likely to represent synapomorphy. Other confounding factors such as differences in evolutionary rates at different sites or amon ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
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