The yeast deletion project, formally the ''Saccharomyces'' Genome Deletion Project, is a project to create data for a near-complete collection of
gene-deletion mutants of the yeast ''
Saccharomyces cerevisiae
''Saccharomyces cerevisiae'' () (brewer's yeast or baker's yeast) is a species of yeast (single-celled fungus microorganisms). The species has been instrumental in winemaking, baking, and brewing since ancient times. It is believed to have been o ...
''. Each strain carries a precise deletion of one of the
gene
In biology, the word gene (from , ; "...Wilhelm Johannsen coined the word gene to describe the Mendelian units of heredity..." meaning ''generation'' or ''birth'' or ''gender'') can have several different meanings. The Mendelian gene is a ba ...
s in the
genome
In the fields of molecular biology and genetics, a genome is all the genetic information of an organism. It consists of nucleotide sequences of DNA (or RNA in RNA viruses). The nuclear genome includes protein-coding genes and non-coding ge ...
. This allows researchers to determine what each gene does by comparing the mutated yeast to the behavior of normal yeast. Gene deletion, or
gene knockout
A gene knockout (abbreviation: KO) is a genetic technique in which one of an organism's genes is made inoperative ("knocked out" of the organism). However, KO can also refer to the gene that is knocked out or the organism that carries the gene kno ...
, is one of the main ways in which the function of genes are discovered. Many of the deletion mutations are sold by the biotech firm
Invitrogen
Invitrogen is one of several brands under the Thermo Fisher Scientific corporation. The product line includes various subbrands of biotechnology products, such as machines and consumables for polymerase chain reaction, reverse transcription, clo ...
.
See also
*
Synthetic genetic array Synthetic genetic array analysis (SGA) is a high-throughput screening, high-throughput technique for exploring synthetic lethality, synthetic lethal and synthetic sick genetic interactions (Synthetic lethality, SSL). SGA allows for the systematic co ...
References
*
External links
* http://www-deletion.stanford.edu/YDPM/YDPM_index.html
* http://www-sequence.stanford.edu/group/yeast_deletion_project/project_desc.html
* http://www-sequence.stanford.edu/group/yeast_deletion_project/references.html
Genome projects
Yeasts
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