A micrograph or photomicrograph is a

photograph A photograph (also known as a photo, image, or picture) is an image created by light falling on a photosensitive surface, usually photographic film or an electronic image sensor, such as a CCD or a CMOS chip. Most photographs are now creat ...

or digital image taken through a

microscope A microscope () is a laboratory instrument used to examine objects that are too small to be seen by the naked eye. Microscopy is the science of investigating small objects and structures using a microscope. Microscopic means being invisibl ...

or similar device to show a

magnified ''Magnified'' is the second album by the American alternative rock band Failure. Production Drummer Robert Gauss departed during the recording of the album; the remaining drum parts were played by John Dargahi and Greg Edwards. ''Magnified'' dif ...

image of an object. This is opposed to a macrograph or photomacrograph, an image which is also taken on a microscope but is only slightly magnified, usually less than 10 times. Micrography is the practice or art of using microscopes to make photographs. A micrograph contains extensive details of microstructure. A wealth of information can be obtained from a simple micrograph like behavior of the material under different conditions, the phases found in the system, failure analysis, grain size estimation, elemental analysis and so on. Micrographs are widely used in all fields of microscopy.

Types

Photomicrograph

A light micrograph or photomicrograph is a micrograph prepared using an

optical microscope The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of micros ...

, a process referred to as ''photomicroscopy''. At a basic level, photomicroscopy may be performed simply by connecting a

camera A camera is an optical instrument that can capture an image. Most cameras can capture 2D images, with some more advanced models being able to capture 3D images. At a basic level, most cameras consist of sealed boxes (the camera body), with a ...

to a microscope, thereby enabling the user to take photographs at reasonably high

magnification Magnification is the process of enlarging the apparent size, not physical size, of something. This enlargement is quantified by a calculated number also called "magnification". When this number is less than one, it refers to a reduction in si ...

. Scientific use began in England in 1850 by Prof Richard Hill Norris

FRSE Fellowship of the Royal Society of Edinburgh (FRSE) is an award granted to individuals that the Royal Society of Edinburgh, Scotland's national academy of science and letters, judged to be "eminently distinguished in their subject". This so ...

for his studies of blood cells. Roman Vishniac was a pioneer in the field of photomicroscopy, specializing in the photography of living creatures in full motion. He also made major developments in light-interruption photography and color photomicroscopy. Photomicrographs may also be obtained using a USB microscope attached directly to a home computer or laptop.

Electron micrograph

An electron micrograph is a micrograph prepared using an

electron microscope An electron microscope is a microscope that uses a beam of accelerated electrons as a source of illumination. As the wavelength of an electron can be up to 100,000 times shorter than that of visible light photons, electron microscopes have a ...

Magnification and micron bars

Micrographs usually have micron bars, or magnification ratios, or both.

Magnification Magnification is the process of enlarging the apparent size, not physical size, of something. This enlargement is quantified by a calculated number also called "magnification". When this number is less than one, it refers to a reduction in si ...

is a ratio between the size of an object on a picture and its real size. Unfortunately, magnification can be a misleading parameter as it depends on the final size of a printed picture and therefore varies with picture size. A ''scale bar'', or ''micron bar'', is a line of known length displayed on a picture. The bar can be used for measurements on a picture. When the picture is resized the bar is also resized making it possible to recalculate the magnification. Ideally, all pictures destined for publication/presentation should be supplied with a scale bar; the magnification ratio is optional. All but one (limestone) of the micrographs presented on this page do not have a micron bar; supplied magnification ratios are likely incorrect, as they were not calculated for pictures at the present size.

Micrography as art

The microscope has been mainly used for scientific discovery. It has also been linked to the arts since its invention in the 17th century. Early adopters of the microscope, such as

Robert Hooke Robert Hooke FRS (; 18 July 16353 March 1703) was an English polymath active as a scientist, natural philosopher and architect, who is credited to be one of two scientists to discover microorganisms in 1665 using a compound microscope that h ...

and Antonie van Leeuwenhoek, were excellent illustrators. Cornelius Varley's graphic microscope made sketching from a microscope easier with a camera-lucida-like mechanism. After the invention of

photography Photography is the visual art, art, application, and practice of creating durable images by recording light, either electronically by means of an image sensor, or chemically by means of a light-sensitive material such as photographic film. It i ...

in the 1820s the microscope was later combined with the camera to take pictures instead of relying on an artistic rendering. Since the early 1970s individuals have been using the microscope as an artistic instrument. Websites and traveling art exhibits such as the

Nikon Small World Nikon Small World (Nikon's Small World) is a photographic contest run by Nikon Instruments and a forum for showcasing the beauty and complexity of life as seen through the light microscope. It serves as a window into a world that can only be ...

and Olympus Bioscapes have featured a range of images for the sole purpose of artistic enjoyment. Some collaborative groups, such as the

Paper Project The Paper Project is a fusion project that blends art, science, and technology into engaging 2D and 3D experiences. About the project The Paper Project is a multidimensional art and science project exploring the microscopic structure of paper u ...

have also incorporated microscopic imagery into

tactile art Tactile may refer to: * Tactile, related to the sense of touch * Haptics (disambiguation) * Tactile (device), a text-to-braille translation device See also * Tangibility Tangibility is the property of being able to be perceived by touch. A c ...

pieces as well as 3D immersive rooms and dance performances. In 2015, photographer and gemologist Danny J. Sanchez photographed mineral and gemstone interiors in works referred to as "otherworldly."

Photomicrography in smartphones

Bellina and Missoni first published a paper in 2009 describing the method of photomicrography in a smartphone with free-hand technique. An operator just need to focus her/his smartphone camera on the eyepiece of a microscope and capture the photo. However, later, various commercial and home-made adapters were introduced to ease focusing. A home-made adapter was also made using scrap materials and a Coca-cola aluminum can.

Gallery

Image:Colpoda400xm2.jpg, Measurements of a large ''

Colpodium ''Colpodium'' is a genus of plants in the grass family, native primarily to Asia but with a few species on certain mountains in Africa. ; SpeciesMill, R. R. 1985. ''Eremopoa, Nephelochloa, Catabrosella, Colpodium, Hyalopoa, Catabrosa, Paracolpod ...

'' at 400x. Image:Amoeba400XM.jpg, Measurements of a large

amoeba An amoeba (; less commonly spelled ameba or amœba; plural ''am(o)ebas'' or ''am(o)ebae'' ), often called an amoeboid, is a type of cell or unicellular organism with the ability to alter its shape, primarily by extending and retracting pseudo ...

at 400x. File:Wilson A. Bentley snowflake, 1890.jpg, Snowflake micrograph by

Wilson Bentley Wilson Alwyn Bentley (February 9, 1865 – December 23, 1931), also known as Snowflake Bentley, was an American meteorologist and photographer, who was the first known person to take detailed photographs of snowflakes and record their featu ...

, 1890. File:Misc pollen.jpg, An image of

pollen Pollen is a powdery substance produced by seed plants. It consists of pollen grains (highly reduced microgametophytes), which produce male gametes (sperm cells). Pollen grains have a hard coat made of sporopollenin that protects the gametop ...

taken from a scanning electron microscope.

References

External links

Shots with a Microscope
– a basic, comprehensive guide to photomicrography
Scientific photomicrographs
– free scientific quality photomicrographs by Doc. RNDr. Josef Reischig, CSc.
Seeing Beyond the Human Eye
Video produced by Off Book (web series)
Solomon C. Fuller bio

Photomicrography by Danny J. Sanchez

Dennis Kunkel Microscopy

Andrew Paul Leonard, APL Microscopic

Cell Centered Database - Montage

Nikon Small World

Olympus Bioscapes

{{Authority control Photographic techniques Microscopy