lactose

Lactose, a disaccharide, is a

sugar

composed of galactose and

glucose

subunits and has the molecular formula C₁₂H₂₂O₁₁. Lactose makes up around 2–8% of

milk

(by weight). The name comes from ' (gen. '), the

Latin

word for milk, plus the suffix ''-ose'' used to name sugars. The compound is a white, water-soluble, non-hygroscopic solid with a mildly sweet taste. It is used in the food industry.

Structure and reactions

Lactose is a disaccharide derived from the condensation of galactose and

glucose

, which form a β-1→4 glycosidic linkage. Its systematic name is β-D-galactopyranosyl-(1→4)-D-glucose. The glucose can be in either the α-pyranose form or the β-pyranose form, whereas the galactose can only have the β-pyranose form: hence α-lactose and β-lactose refer to the anomeric form of the glucopyranose ring alone.
Detection reactions for lactose are the Woehlk- and Fearon's test. Both can be easily used in school experiments to visualise the different lactose content of different dairy products such as whole milk, lactose free milk, yoghurt, buttermilk, coffee creamer, sour cream, kefir etc.

Lactose is hydrolysed to glucose and galactose, isomerised in alkaline solution to

lactulose

, and

catalytically

hydrogenated to the corresponding polyhydric alcohol,

lactitol

. Lactulose is a commercial product, used for treatment of constipation.

Occurrence and isolation

Lactose composes about 2–8% of milk by weight. Several million tons are produced annually as a by-product of the dairy industry.

Whey

or milk plasma is the liquid remaining after milk is curdled and strained, for example in the production of

cheese

. Whey is made up of 6.5% solids, of which 4.8% is lactose, which is purified by crystallisation.
Industrially, lactose is produced from whey permeate – that is whey filtrated for all major

protein

s. The protein fraction is used in infant nutrition and sports nutrition while the permeate can be evaporated to 60–65% solids and crystallized while cooling. Lactose can also be isolated by dilution of whey with

ethanol

.

Dairy products such as milk

yogurt

and

cheese

contain very little lactose. Lactase is used as the bacteria used to make them consume lactose during the manufacturing process.

Metabolism

Infant mammals nurse on their mothers to drink milk, which is rich in lactose. The intestinal villi secrete the

enzyme

lactase

(β-D-galactosidase) to digest it. This enzyme cleaves the lactose molecule into its two subunits, the simple

sugar

s

glucose

and galactose, which can be absorbed. Since lactose occurs mostly in milk, in most mammals, the production of lactase gradually decreases with maturity due to a lack of continuing consumption.

Many people with ancestry in

Europe

, West Asia,

South Asia

, the

Sahel

belt in

West Africa

, East Africa and a few other parts of

Central Africa

maintain lactase production into adulthood. In many of these areas, milk from mammals such as

cattle

,

goats

, and

sheep

is used as a large source of food. Hence, it was in these regions that genes for lifelong lactase production first

evolved

. The genes of adult lactose tolerance have evolved independently in various ethnic groups. By descent, more than 70% of western Europeans can digest lactose as adults, compared with less than 30% of people from areas of Africa, eastern and south-eastern Asia and Oceania. In people who are lactose intolerant, lactose is not broken down and provides food for gas-producing gut flora, which can lead to diarrhea, bloating, flatulence, and other gastrointestinal symptoms.

Biological properties

The

sweetness

of lactose is 0.2 to 0.4, relative to 1.0 for

sucrose

. For comparison, the sweetness of

glucose

is 0.6 to 0.7, of

fructose

is 1.3, of galactose is 0.5 to 0.7, of

maltose

is 0.4 to 0.5, of

sorbose

is 0.4, and of

xylose

is 0.6 to 0.7.

When lactose is completely digested in the

small intestine

, its caloric value is 4 kcal/g, or the same as that of other carbohydrates. However, lactose is not always fully digested in the small intestine. Depending on ingested dose, combination with meals (either solid or liquid), and

lactase

activity in the

intestine

s, the caloric value of lactose ranges from 2 to 4 kcal/g. Undigested lactose acts as dietary fiber. It also has positive effects on absorption of

mineral

s, such as

calcium

and

magnesium

.

The glycemic index of lactose is 46 to 65. For comparison, the glycemic index of

glucose

is 100 to 138, of

sucrose

is 68 to 92, of

maltose

is 105, and of

fructose

is 19 to 27.

Lactose has relatively low cariogenicity among

sugar

s. This is because it is not a substrate for dental plaque formation and it is not rapidly

fermented

by

oral

bacteria

. The buffering capacity of milk also reduces the cariogenicity of lactose.

Applications

Its mild flavor and easy handling properties have led to its use as a carrier and stabiliser of aromas and pharmaceutical products. Lactose is not added directly to many foods, because its solubility is less than that of other sugars commonly used in food. Infant formula is a notable exception, where the addition of lactose is necessary to match the composition of human milk.

Lactose is not fermented by most

yeast

during brewing, which may be used to advantage. For example, lactose may be used to sweeten stout beer; the resulting beer is usually called a milk stout or a cream stout.

Yeast belonging to the genus ''Kluyveromyces'' have a unique industrial application as they are capable of fermenting lactose for ethanol production. Surplus lactose from the whey by-product of dairy operations is a potential source of alternative energy.

Another significant lactose use is in the pharmaceutical industry. Lactose is added to tablet and capsule drug products as an ingredient because of its physical and functional properties. For similar reasons it can be used to dilute illicit drugs such as cocaine or heroin.

History

The first crude isolation of lactose, by Italian physician Fabrizio Bartoletti (1576–1630), was published in 1633. In 1700, the Venetian pharmacist Lodovico Testi (1640–1707) published a booklet of testimonials to the power of milk sugar (''saccharum lactis'') to relieve, among other ailments, the symptoms of arthritis. In 1715, Testi's procedure for making milk sugar was published by Antonio Vallisneri. Lactose was identified as a

sugar

in 1780 by

Carl Wilhelm Scheele

.

In 1812, Heinrich Vogel (1778–1867) recognized that glucose was a product of hydrolyzing lactose. In 1856,

Louis Pasteur

crystallized the other component of lactose, galactose. By 1894,

Emil Fischer

had established the configurations of the component sugars.

Lactose was named by the French chemist Jean Baptiste André Dumas (1800–1884) in 1843.
In 1856,

Louis Pasteur

named galactose "lactose". In 1860,

Marcellin Berthelot

renamed it "galactose", and transferred the name "lactose" to what is now called lactose.Marcellin Berthelot, ''Chimie organique fondée sur la synthèse'' rganic chemistry based on synthesis(Paris, France: Mallet-Bachelier, 1860), vol. 2
pp. 248–249
an
pp. 268–270.
/ref> It has a formula of C₁₂H₂₂O₁₁ and the hydrate formula C₁₂H₂₂O₁₁·H₂O, making it an

isomer

of

sucrose

.

See also

*Lactose intolerance
*

Nectar

*Sugars in wine
*

lac operon

*

Lactic acid

References

External links

*

{{Authority control

Disaccharides
Excipients
Sugar substitutes