Further development
The physical and functional separation of the inner cell mass from the trophectoderm (TE) is a special feature of mammalian development and is the first cell lineage specification in these embryos. Following fertilization in the oviduct, the mammalian embryo undergoes a relatively slow round of cleavages to produce an eight-cell morula. Each cell of the morula, called a blastomere, increases surface contact with its neighbors in a process called compaction. This results in a polarization of the cells within the morula, and further cleavage yields aRegulation of cellular specification
Since segregation of pluripotent cells of the inner cell mass from the remainder of the blastocyst is integral to mammalian development, considerable research has been performed to elucidate the corresponding cellular and molecular mechanisms of this process. There is primary interest in which transcription factors and signaling molecules direct blastomere asymmetric divisions leading to what are known as inside and outside cells and thus cell lineage specification. However, due to the variability and regulative nature of mammalian embryos, experimental evidence for establishing these early fates remains incomplete. At the transcription level, the transcription factors Oct4, Nanog, Cdx2, and Tead4 have all been implicated in establishing and reinforcing the specification of the ICM and the TE in early mouse embryos. *Oct4: ''Oct4'' is expressed in the ICM and participate in maintaining its pluripotency, a role that has been recapitulated in ICM derived mouse embryonic stem cells.Nichols J, Zevnik B, Anastassiadis K, Niwa H, Klewe-Nebenius D, Chambers I, Sch€oler H, Smith A. 1998. Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4. Cell 95:379–391. ''Oct4'' genetic knockout cells both in vivo and in culture display TE morphological characteristics. It has been shown that one transcriptional target of Oct4 is the ''Fgf4'' gene. This gene normally encodes a ligand secreted by the ICM, which induces proliferation in the adjacent polar TE. *Nanog: ''Nanog'' is also expressed in the ICM and participates in maintaining its pluripotency. In contrast with ''Oct4'', studies of ''Nanog''-null mice do not show the reversion of the ICM to a TE-like morphology, but demonstrate that loss of ''Nanog'' prevents the ICM from generating primitive endoderm. *Cdx2: ''Cdx2'' is strongly expressed in the TE and is required for maintaining its specification. Knockout mice for the ''Cdx2'' gene undergo compaction, but lose the TE epithelial integrity during the late blastocyst stage. Furthermore, ''Oct4'' expression is subsequently raised in these TE cells, indicating Cdx2 plays a role in suppressing ''Oct4'' in this cell lineage. Moreover, embryonic stem cells can be generated from ''Cdx2''-null mice, demonstrating that Cdx2 is not essential for ICM specification.Strumpf D, Mao CA, Yamanaka Y, Ralston A, Chawengsaksophak K, Beck F, Rossant J. 2005. Cdx2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst. Development 132:2093–2102. *Tead4: Like ''Cdx2'', ''Tead4'' is required for TE function, although the transcription factor is expressed ubiquitously. ''Tead4''-null mice similarly undergo compaction, but fail to generate the blastocoel cavity. Like ''Cdx2''-null embryos, the Tead4-null embryos can yield embryonic stem cells, indicating that Tead4 is dispensable for ICM specification. Recent work has shown that ''Tead4'' may help to upregulate Cdx2 in the TE and its transcriptional activity depends on the coactivator Yap. Yap's nuclear localization in outside cells allows it to contribute to TE specificity, whereas inside cells sequester Yap in the cytoplasm through a phosphorylation event. Together these transcription factors function in a positive feedback loop that strengthens the ICM to TE cellular allocation. Initial polarization of blastomeres occurs at the 8-16 cell stage. An apical-basolateral polarity is visible through the visualization of apical markers such as Par3, Par6, and aPKC as well as the basal marker E-Cadherin. The establishment of such a polarity during compaction is thought to generate an environmental identity for inside and outside cells of the embryo. Consequently, stochastic expression of the above transcription factors is amplified into a feedback loop that specifies outside cells to a TE fate and inside cells to an ICM fate. In the model, an apical environment turns on ''Cdx2'', which upregulates its own expression through a downstream transcription factor, Elf5. In concert with a third transcription factor, Eomes, these genes act to suppress pluripotency genes like ''Oct4'' and ''Nanog'' in the outside cells. Thus, TE becomes specified and differentiates. Inside cells, however, do not turn on the ''Cdx2'' gene, and express high levels of ''Oct4'', ''Nanog'', and ''Sox2''. These genes suppress ''Cdx2'' and the inside cells maintain pluripotency generate the ICM and eventually the rest of the embryo proper. Although this dichotomy of genetic interactions is clearly required to divide the blastomeres of the mouse embryo into both the ICM and TE identities, the initiation of these feedback loops remains under debate. Whether they are established stochastically or through an even earlier asymmetry is unclear, and current research seeks to identify earlier markers of asymmetry. For example, some research correlates the first two cleavages during embryogenesis with respect to the prospective animal and vegetal poles with ultimate specification. The asymmetric division of epigenetic information during these first two cleavages, and the orientation and order in which they occur, may contribute to a cell's position either inside or outside the morula.Stem cells
Blastomeres isolated from the ICM of mammalian embryos and grown in culture are known as embryonic stem (ES) cells. These pluripotent cells, when grown in a carefully coordinated media, can give rise to all three germ layers (ectoderm, endoderm, and mesoderm) of the adult body.Robertson, Elizabeth , et al. Germ-line transmission of genes introduced into cultured pluripotential cells by retroviral vector. Nature 323, 445 – 448 (2 October 1986) For example, the transcription factor LIF4 is required for mouse ES cells to be maintained in vitro.Smith AG, Heath JK, Donaldson DD, Wong GG, Moreau J, Stahl M and Rogers D (1988) Inhibition of pluripotential embryonic stem cell differentiation by purified polypeptides. Nature, 336, 688–690 Blastomeres are dissociated from an isolated ICM in an early blastocyst, and their transcriptional code governed by ''Oct4'', ''Sox2'', and ''Nanog'' helps maintain an undifferentiated state. One benefit to the regulative nature in which mammalian embryos develop is the manipulation of blastomeres of the ICM to generate knockout mice. In mouse, mutations in a gene of interest can be introduced retrovirally into cultured ES cells, and these can be reintroduced into the ICM of an intact embryo. The result is a chimeric mouse, which develops with a portion of its cells containing the ES cell genome. The aim of such a procedure is to incorporate the mutated gene into the germ line of the mouse such that its progeny will be missing one or both alleles of the gene of interest. Geneticists widely take advantage of this ICM manipulation technique in studying the function of genes in the mammalian system.Additional images
See also
*References
{{DEFAULTSORT:Inner Cell Mass Developmental biology