A ganglioside is a molecule composed of a
glycosphingolipid (
ceramide
Ceramides are a family of waxy lipid molecules. A ceramide is composed of N-acetylsphingosine and a fatty acid. Ceramides are found in high concentrations within the cell membrane of eukaryotic cells, since they are component lipids that make u ...
and
oligosaccharide) with one or more
sialic acids (e.g.
''N''-acetylneuraminic acid, NANA) linked on the
sugar chain. NeuNAc, an acetylated derivative of the carbohydrate sialic acid, makes the head groups of gangliosides anionic at
pH 7, which distinguishes them from
globosides.
The name ''ganglioside'' was first applied by the German scientist
Ernst Klenk
Ernst Klenk (14 October 1896, Pfalzgrafenweiler – 29 December 1971, Cologne) was a German biochemist, known as a pioneer in research on biolipids, their metabolism, and diseases caused by biolipid disorders.
Biography
Klenk's father had a farm ...
in 1942 to lipids newly isolated from
ganglion cells of the brain. More than 60 gangliosides are known, which differ from each other mainly in the position and number of
NANA
Nana, Nanna, Na Na or NANA may refer to:
People and fictional characters
* Nana (given name), including a list of people and characters with the given name
* Nana (surname), including a list of people and characters with the surname
* Nana ( ...
residue
Residue may refer to:
Chemistry and biology
* An amino acid, within a peptide chain
* Crop residue, materials left after agricultural processes
* Pesticide residue, refers to the pesticides that may remain on or in food after they are applie ...
s. It is a component of the cell
plasma membrane that modulates cell
signal transduction events, and appears to concentrate in
lipid rafts.
Recently, gangliosides have been found to be highly important molecules in
immunology
Immunology is a branch of medicineImmunology for Medical Students, Roderick Nairn, Matthew Helbert, Mosby, 2007 and biology that covers the medical study of immune systems in humans, animals, plants and sapient species. In such we can see ther ...
. Natural and semisynthetic gangliosides are considered possible therapeutics for neurodegenerative disorders.
Location
Gangliosides are present and concentrated on cell surfaces, with the two hydrocarbon chains of the ceramide moiety embedded in the plasma membrane and the oligosaccharides located on the extracellular surface, where they present points of recognition for extracellular molecules or surfaces of neighboring cells. They are found predominantly in the nervous system where they constitute 6% of all phospholipids.
Function
The oligosaccharide groups on gangliosides extend well beyond the surfaces of the cell membranes, and act as distinguishing surface markers that can serve as specific determinants in cellular recognition and cell-to-cell communication. These carbohydrate head groups also act as specific receptors for certain
pituitary glycoprotein hormones
A hormone (from the Greek participle , "setting in motion") is a class of signaling molecules in multicellular organisms that are sent to distant organs by complex biological processes to regulate physiology and behavior. Hormones are required fo ...
and certain bacterial protein toxins such as
cholera toxin
Cholera toxin (also known as choleragen and sometimes abbreviated to CTX, Ctx or CT) is AB5 multimeric protein complex secreted by the bacterium ''Vibrio cholerae''. CTX is responsible for the massive, watery diarrhea characteristic of cholera i ...
.
The functions of gangliosides as specific determinants suggest its important role in the growth and differentiation of tissues as well as in
carcinogenesis
Carcinogenesis, also called oncogenesis or tumorigenesis, is the formation of a cancer, whereby normal cells are transformed into cancer cells. The process is characterized by changes at the cellular, genetic, and epigenetic levels and abnor ...
. It has been found that tumor formation can induce the synthesis of a new complement of ganglioside, and very low concentrations of a specific ganglioside can induce differentiation of
cultured neuronal tumor cells.
Common gangliosides
* One
NANA
Nana, Nanna, Na Na or NANA may refer to:
People and fictional characters
* Nana (given name), including a list of people and characters with the given name
* Nana (surname), including a list of people and characters with the surname
* Nana ( ...
("M")
**
GM1
GM1 (monosialotetrahexosylganglioside) the "prototype" ganglioside, is a member of the ganglio series of gangliosides which contain one sialic acid residue. GM1 has important physiological properties and impacts neuronal plasticity and repair ...
**
GM2
**
GM3
GM3 (monosialodihexosylganglioside) is a type of ganglioside. The letter G refers to ganglioside, and M is for monosialic acid as it has one sialic acid only. The numbering is based on its relative mobility in electrophoresis among other monosi ...
* Two NANAs ("D")
**
GD1a
**
GD1b
**
GD2
GD2 is a disialoganglioside expressed on tumors of neuroectodermal origin, including human neuroblastoma and melanoma, with highly restricted expression on normal tissues, principally to the cerebellum and peripheral nerves in humans.
The rela ...
**
GD3
* Three NANAs ("T")
**
GT1b
**
GT3
* Four NANAs ("Q")
**
GQ1
Structures of the common gangliosides
GM2-1 = aNeu5Ac(2-3)bDGalp(1-?)bDGalNAc(1-?)bDGalNAc(1-?)bDGlcp(1-1)Cer
GM3 = aNeu5Ac(2-3)bDGalp(1-4)bDGlcp(1-1)Cer
GM2,GM2a(?) = N-Acetyl-D-galactose-beta-1,4-
-Acetylneuraminidate- alpha-2,3-Galactose-beta-1,4-glucose-alpha-ceramide
GM2b(?) = aNeu5Ac(2-8)aNeu5Ac(2-3)bDGalp(1-4)bDGlcp(1-1)Cer
GM1,GM1a = bDGalp(1-3)bDGalNAc
Neu5Ac(2-3)DGalp(1-4)bDGlcp(1-1)Cer
asialo-GM1,GA1 = bDGalp(1-3)bDGalpNAc(1-4)bDGalp(1-4)bDGlcp(1-1)Cer
asialo-GM2,GA2 = bDGalpNAc(1-4)bDGalp(1-4)bDGlcp(1-1)Cer
GM1b = aNeu5Ac(2-3)bDGalp(1-3)bDGalNAc(1-4)bDGalp(1-4)bDGlcp(1-1)Cer
GD3 = aNeu5Ac(2-8)aNeu5Ac(2-3)bDGalp(1-4)bDGlcp(1-1)Cer
GD2 = bDGalpNAc(1-4)
Neu5Ac(2-8)aNeu5Ac(2-3)DGalp(1-4)bDGlcp(1-1)Cer
GD1a = aNeu5Ac(2-3)bDGalp(1-3)bDGalNAc(1-4)
Neu5Ac(2-3)DGalp(1-4)bDGlcp(1-1)Cer
GD1alpha = aNeu5Ac(2-3)bDGalp(1-3)bDGalNAc(1-4)
Neu5Ac(2-6)
''N''-Acetylneuraminic acid (Neu5Ac or NANA) is the predominant sialic acid found in human cells, and many mammalian cells. Other forms, such as N-Glycolylneuraminic acid, may also occur in cells.
This residue is negatively charged at physiolog ...
DGalp(1-4)bDGlcp(1-1)Cer
GD1b = bDGalp(1-3)bDGalNAc(1-4)
Neu5Ac(2-8)aNeu5Ac(2-3)DGalp(1-4)bDGlcp(1-1)Cer
GT1a = aNeu5Ac(2-8)aNeu5Ac(2-3)bDGalp(1-3)bDGalNAc(1-4)
Neu5Ac(2-3)DGalp(1-4)bDGlcp(1-1)Cer
GT1,GT1b = aNeu5Ac(2-3)bDGalp(1-3)bDGalNAc(1-4)
Neu5Ac(2-8)aNeu5Ac(2-3)DGalp(1-4)bDGlcp(1-1)Cer
OAc-GT1b = aNeu5Ac(2-3)bDGalp(1-3)bDGalNAc(1-4)aXNeu5Ac9Ac(2-8)aNeu5Ac(2-3)]bDGalp(1-4)bDGlcp(1-1)Cer
GT1c = bDGalp(1-3)bDGalNAc(1-4)
Neu5Ac(2-8)aNeu5Ac(2-8)aNeu5Ac(2-3)DGalp(1-4)bDGlcp(1-1)Cer
GT3 = aNeu5Ac(2-8)aNeu5Ac(2-8)aNeu5Ac(2-3)bDGal(1-4)bDGlc(1-1)Cer
GQ1b = aNeu5Ac(2-8)aNeu5Ac(2-3)bDGalp(1-3)bDGalNAc(1-4)
Neu5Ac(2-8)aNeu5Ac(2-3)DGalp(1-4)bDGlcp(1-1)Cer
GGal = aNeu5Ac(2-3)bDGalp(1-1)Cer
where
* aNeu5Ac = N-acetyl-alpha-neuraminic acid
* aNeu5Ac9Ac = N-acetyl-9-O-acetylneuraminic acid
* bDGalp = beta-D-galactopyranose
* bDGalpNAc = N-acetyl-beta-D-galactopyranose
* bDGlcp = beta-D-glucopyranose
* Cer = ceramide (general N-acylated sphingoid)
Pathology
Gangliosides are continuously synthesized and degraded in cells. They are degraded to ceramides by sequential removal of sugar units in the oligosaccharide group, catalyzed by a set of highly specific
lysosomal
A lysosome () is a membrane-bound organelle found in many animal cells. They are spherical vesicles that contain hydrolytic enzymes that can break down many kinds of biomolecules. A lysosome has a specific composition, of both its membrane pro ...
enzymes. Mutations in genes coding for these enzymes leads to the accumulation of partially broken down gangliosides in lysosomes, which results in a group of diseases called
gangliosidosis. For example, the fatal
Tay–Sachs disease
Tay–Sachs disease is a genetic disorder that results in the destruction of nerve cells in the brain and spinal cord. The most common form is infantile Tay–Sachs disease, which becomes apparent around three to six months of age, with the baby ...
arises as a genetic defect which leads to no functional
hexosaminidase A produced, causing GM2 to accumulate in lysosomes. Ultimately the
ganglion cells in the nervous system swell enormously, disturbing the normal functions of neurons.
Gangliosides are also involved in several diseases:
*
Influenza, in which
haemagglutinin of
influenza virus exploits certain gangliosides to enter and infect the cells expressing them.
*
Guillain–Barré syndrome, which has been linked to the production of anti-ganglioside
antibodies
An antibody (Ab), also known as an immunoglobulin (Ig), is a large, Y-shaped protein used by the immune system to identify and neutralize foreign objects such as pathogenic bacteria and viruses. The antibody recognizes a unique molecule of the ...
.
*
Cholera
Cholera is an infection of the small intestine by some strains of the bacterium ''Vibrio cholerae''. Symptoms may range from none, to mild, to severe. The classic symptom is large amounts of watery diarrhea that lasts a few days. Vomiting and ...
*
Tetanus
Tetanus, also known as lockjaw, is a bacterial infection caused by ''Clostridium tetani'', and is characterized by muscle spasms. In the most common type, the spasms begin in the jaw and then progress to the rest of the body. Each spasm usually ...
*
Botulism
Botulism is a rare and potentially fatal illness caused by a toxin produced by the bacterium ''Clostridium botulinum''. The disease begins with weakness, blurred vision, feeling tired, and trouble speaking. This may then be followed by weaknes ...
*
Leprosy
Leprosy, also known as Hansen's disease (HD), is a long-term infection by the bacteria '' Mycobacterium leprae'' or '' Mycobacterium lepromatosis''. Infection can lead to damage of the nerves, respiratory tract, skin, and eyes. This nerve dam ...
*
Obesity, where inadequate ganglioside expression in mediobasal hypothalamic neurons deregulates neuronal leptin and insulin signaling.
References
External links
*
Overview of gangliosides at lipidlibrary.co.uk
{{Autoantigens
Glycolipids