cycloheximide

Cycloheximide is a naturally occurring fungicide produced by the bacterium '' Streptomyces griseus''. Cycloheximide exerts its effects by interfering with the translocation step in protein synthesis (movement of two tRNA molecules and mRNA in relation to the ribosome), thus blocking eukaryotic translational elongation. Cycloheximide is widely used in biomedical research to inhibit protein synthesis in eukaryotic cells studied ''in vitro'' (''i.e.'' outside of organisms). It is inexpensive and works rapidly. Its effects are rapidly reversed by simply removing it from the culture medium.

Due to significant toxic side effects, including DNA damage, teratogenesis, and other reproductive effects (including birth defects and toxicity to sperm), cycloheximide is generally used only in ''in vitro'' research applications, and is not suitable for human use as a therapeutic compound. Although it has been used as a fungicide in agricultural applications, this application is now decreasing as the health risks have become better understood.

Because cycloheximide rapidly breaks down in a basic environment, decontamination of work surfaces and containers can be achieved by washing with a non-harmful alkali solution such as soapy water or aqueous sodium bicarbonate.

It is classified as an extremely hazardous substance in the United States as defined in Section 302 of the U.S. Emergency Planning and Community Right-to-Know Act (42 U.S.C. 11002), and is subject to strict reporting requirements by facilities which produce, store, or use it in significant quantities.

Discovery

Cycloheximide was reported in 1946 by Alma Joslyn Whiffen-Barksdale at the Upjohn Company.

Experimental applications

Cycloheximide can be used as an experimental tool in molecular biology to determine the half-life of a protein. Treating cells with cycloheximide in a time-course experiment followed by western blotting of the cell lysates for the protein of interest can show differences in protein half-life. Cycloheximide treatment provides the ability to observe the half-life of a protein without confounding contributions from transcription or translation.

Mitochondrial protein synthesis is resistant to inhibition by cycloheximide. On the other hand chloramphenicol inhibits mitochondrial (and bacterial) protein synthesis, but synthesis on cytoplasmic ribosomes is resistant. Before genomes were available, these inhibitors were used to determine which mitochondrial proteins were synthesized in the mitochondria from mitochondrial genes.

Cycloheximide is used as a plant growth regulator to stimulate ethylene production. It is used as a rodenticide and other animal pesticide. It is also used in media to detect unwanted bacteria in beer fermentation by suppressing yeasts and molds growth in test medium.

The translational elongation freezing properties of cycloheximide are also used for ribosome profiling / translational profiling. Translation is halted via the addition of cycloheximide, and the DNA/RNA in the cell is then nuclease treated. The ribosome-bound parts of RNA can then be sequenced.

Cycloheximide has also been used to make isolation of bacteria from environmental samples easier.

Spectrum of fungal susceptibility

Cycloheximide has been used to isolate dermatophytes and inhibit the growth of fungi in brewing test media. The following represents susceptibility data for a few commonly targeted fungi:
* ''Candida albicans'': 12.5 μg/ml
* ''Mycosphaerella graminicola'': 47.2 μg/ml – 85.4 μg/ml
* ''Saccharomyces cerevisiae'': 0.05 μg/ml – 1.6 μg/ml
* ''Neoscytalidium dimidiatum'' is an Athlete's foot like infection resistant to most antifungals but is rather sensitive to cycloheximide, so, it should be cultured in a medium free of cycloheximide.

See also

* Acetoxycycloheximide

References

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Secondary alcohols
Protein synthesis inhibitors
Fungicides
Glutarimides
Ketones