Zonula occludens-1 ZO-1, also known as Tight junction protein-1 is a 220-kD
peripheral membrane protein
Peripheral membrane proteins, or extrinsic membrane proteins, are membrane proteins that adhere only temporarily to the biological membrane with which they are associated. These proteins attach to integral membrane proteins, or penetrate the periph ...
that is encoded by the ''TJP1''
gene
In biology, the word gene (from , ; "...Wilhelm Johannsen coined the word gene to describe the Mendelian units of heredity..." meaning ''generation'' or ''birth'' or ''gender'') can have several different meanings. The Mendelian gene is a ba ...
in humans.
It belongs to the family of ''zonula occludens proteins'' (ZO-1, ZO-2, and ZO-3), which are
tight junction
Tight junctions, also known as occluding junctions or ''zonulae occludentes'' (singular, ''zonula occludens''), are multiprotein junctional complexes whose canonical function is to prevent leakage of solutes and water and seals between the epith ...
-associated
protein
Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues. Proteins perform a vast array of functions within organisms, including catalysing metabolic reactions, DNA replication, respo ...
s and of which, ZO-1 is the first to be cloned. It was first isolated in 1986 by Stevenson and Goodenough using a monoclonal antibody raised in rodent liver to recognise a 225-kD polypeptide in whole liver homogenates and in tight junction-enriched membrane fractions. It has a role as a
scaffold protein
In biology, scaffold proteins are crucial regulators of many key signalling pathways. Although scaffolds are not strictly defined in function, they are known to interact and/or bind with multiple members of a signalling pathway, tethering them in ...
which cross-links and anchors Tight Junction (TJ) strand proteins, which are fibril-like structures within the lipid bilayer, to the actin cytoskeleton.
Function
This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell–cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene.
Interactions
Tight junction protein 1 has been shown to
interact
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with:
*
F11 receptor
Junctional adhesion molecule A is a protein that in humans is encoded by the ''F11R'' gene. It has also been designated as CD321 (cluster of differentiation 321).
Function
Tight junctions represent one mode of cell-to-cell adhesion in epithelia ...
,
*
GJA3
Gap junction alpha-3 protein is a protein that in humans is encoded by the ''GJA3'' gene.
Interactions
GJA3 has been shown to interact
Advocates for Informed Choice, doing business as, dba interACT or interACT Advocates for Intersex Youth, is ...
,
*
GJA8
Gap junction alpha-8 protein is a protein that in humans is encoded by the ''GJA8'' gene. It is also known as connexin 50.
Related gene problems
*1q21.1 deletion syndrome
*1q21.1 duplication syndrome
*microphthalmia and other vision pathologie ...
,
[
* ]Gap junction protein, alpha 1
Gap junction alpha-1 protein (GJA1), also known as connexin 43 (Cx43), is a protein that in humans is encoded by the ''GJA1'' gene on chromosome 6. As a connexin, GJA1 is a component of gap junctions, which allow for gap junction intercellular co ...
,KIRREL
Kin of IRRE-like protein 1, also known as NEPH1, is a protein that in humans is encoded by the ''KIRREL'' gene.
Function
NEPH1 is a member of the NEPH protein family, which includes NEPH2 ( KIRREL3, MIMbr>607761 and NEPH3 ( KIRREL2, MIMbr>6077 ...
,MLLT4
Afadin is a protein that in humans is encoded by the ''AFDN'' gene.
Function
Afadin is a Ras (see HRAS; MIM 190020) target that regulates cell–cell adhesions downstream of Ras activation. It is fused with MLL (MIM 159555) in leukemias caused ...
,Occludin
Occludin is an enzyme ( EC 1.6) that oxidizes NADH. It was first identified in epithelial cells as a 65 kDa integral plasma-membrane protein localized at the tight junctions. Together with Claudins, and zonula occludens-1 (ZO-1), occludin has bee ...
,TJP3
Tight junction protein ZO-3 is a protein that in humans is encoded by the ''TJP3'' gene
In biology, the word gene (from , ; "... Wilhelm Johannsen coined the word gene to describe the Mendelian units of heredity..." meaning ''generation'' ...
,Tight junction protein 2
Tight junction protein ZO-2 is a protein that in humans is encoded by the ''TJP2'' gene.
Tight junction proteins (TJPs) belong to a family of membrane-associated guanylate kinase (MAGUK) homologs that are involved in the organization of epithelia ...
.
See also
* Tight junction
Tight junctions, also known as occluding junctions or ''zonulae occludentes'' (singular, ''zonula occludens''), are multiprotein junctional complexes whose canonical function is to prevent leakage of solutes and water and seals between the epith ...
References
Further reading
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External links
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{{PDB Gallery, geneid=7082