V8 Medium
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V8 medium is a kind of
agar Agar ( or ), or agar-agar, is a jelly-like substance consisting of polysaccharides obtained from the cell walls of some species of red algae, primarily from ogonori (''Gracilaria'') and "tengusa" (''Gelidiaceae''). As found in nature, agar is ...
medium Medium may refer to: Science and technology Aviation *Medium bomber, a class of war plane * Tecma Medium, a French hang glider design Communication * Media (communication), tools used to store and deliver information or data * Medium of ...
which is used to cultivate the spore of
Phytophthora sojae ''Phytophthora sojae'' is an oomycete and a soil-borne plant pathogen that causes stem and root rot of soybean. This is a prevalent disease in most soybean growing regions, and a major cause of crop loss. In wet conditions the pathogen produces z ...
. Phytophthora sojae causes
soybean The soybean, soy bean, or soya bean (''Glycine max'') is a species of legume native to East Asia, widely grown for its edible bean, which has numerous uses. Traditional unfermented food uses of soybeans include soy milk, from which tofu an ...
blight Blight refers to a specific symptom affecting plants in response to infection by a pathogenic organism. Description Blight is a rapid and complete chlorosis, browning, then death of plant tissues such as leaves, branches, twigs, or floral organ ...
(Phytophthora root rot of soybeans). Soybean blight can affect the output and quality of soybeans seriously. The spore of phytophthora sojae is difficult to culture in
potato dextrose agar Potato dextrose agar (BAM Media M127
from the U.S.
; it is generally cultured by V8 medium and
lima bean agar Lima bean agar (LBA) is an agar medium which is used to cultivate the spores of ''Phytophthora sojae''. ''Phytophthora sojae'' causes soybean blight (Phytophthora root rot of soybeans). Soybean blight can affect the output and quality of soybeans ...
at home and abroad. V8 medium can cultivate, separate, reproduce and conserve many kinds spore of phytophthora sojae, but it is not suitable for cultivating the pathogen of potato late blight.


References

* Sinclair, J.B.; Dhingra, O.D. (1995). Basic Plant Pathology Methods. Taylor & Francis. p. 36. . * Santamaria, L. (2007). Evaluation of Lima Bean (Phaseolus Lunatus) Germplasm for Resistance to Downy Mildew, and Epidemiological and Biological Studies of Its Causal Agent, Phytophthora Phaseoli. University of Delaware. pp. 39–40. {{ISBN, 978-0-549-39600-0. Cell culture media