USP6

Ubiquitin carboxyl-terminal hydrolase 6 (USB6), also termed TRE17 and Tre-2, is a deubiquitinating enzyme that in humans is encoded by the hominid (i.e. found only in primates) ''USP6'' gene located at band 13.2 on the short (i.e. "p") arm of chromosome 17 (notated as 17p13.2). Deubiquitinating enzymes (DUBs) are enzymes that act within cells to remove ubiquitins from various functionally important proteins. Ubiquitin enzymes add ubiquitin to these proteins and thereby regulate their cellular location, alter their activity, and/or promote their degradation. By deubiquitinating these proteins, DUBs counter the effects of the ubiquinating enzymes and contribute to regulating the actions of the targeted proteins. In normal adult tissues, USP6 is highly expressed in testicle tissue, modestly expressed in ovarian tissue, and absent or minimally expressed in other tissues. It is also highly expressed in fetal brain tissue. The specific functions of USP6 are poorly defined primarily because its presence is restricted to primates: there are no available animal models to determine the effects of its deletion, although some studies suggest that UPSP6 contributes to normal brain development. In all events, USP6 has gained wide interest because of its abnormally increased expression by the neoplastic cells in various tumors derived from mesenchymal tissue.

The ''USP6'' gene associated with tumors is part of a fusion gene. Fusion genes are abnormal and potentially tumor-inducing genes formed by mergers between parts of two different genes as a result of large scale gene mutations such as chromosomal translocations, interstitial deletions, or inversions. For example, the ''USP6-COL1A1'' fusion gene is formed by a translocation between part of the ''USP6'' gene located at band 13.2 on the p arm of chromosome 17 and the ''COL1A1'' gene located at band 21.33 on the q arm of this same chromosome. The ''USP6'' gene has been documented to fuse with any one of scores of other genes and in doing so (as tested in many cases) create a fusion gene that is overproduced and contains high levels of deubiquitinating activity. Studies suggest that ''USP6''-containing fusion genes cause or at least contribute to tumor development by inappropriately activating multiple cell signaling pathways including the Wnt signaling pathway, one of the JAK-STAT signaling pathways (i.e. the Jak1-STAT3 pathway), the c-Jun signaling pathway, and the NF-κB signaling pathway. All of these pathways, when inappropriately activated, have been implicated in promoting the development of tumors and cancers. The World Health Organization, 2021, classification of Tumors of Soft Tissue suggests that ''USP6''-containing fusion protein-associated tumors are typically benign and usually self-limited in their growth. Furthermore, high levels of USP6 activity may act to suppress rather than promote tumor development in Ewing sarcoma, a tumor which has ''USP''-containing fusion genes in ~1/3 of cases.

Tumor types that are associated with ''USP6''-containing fusion genes and appear to promote their development and/or growth include:
* Aneurysmal bone cysts: Found in 59% to 75%
* Fibroma of tendon sheaths: Found in 6 out of 9 (67%) tested cases. of cases.
* Giant cell reparative granuloma (GCRG): GCRG are regarded as variants of aneurysmal bone cysts that occur in sites other than the jaw. An ''USP6''-containing fusion gene was found in 8 of 9 (89%) GCRG tumors that were located in the hands or feet.
* Nodular fasciitis: Found in close to 90% of all cases.
* Myositis ossificans and fibro-osseous pseudotumor of digits: The World Health Organization, 2021, classified these two tumors as a single entity in the family of Fibroblastic and myofibroblastic tumors. ''USP6''-containing fusion genes has been found in 8 of 9 (89%) tested cases of these tumors.

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{{Posttranslational modification