''Trebouxia'' is a unicellular

green alga The green algae (singular: green alga) are a group consisting of the Prasinodermophyta and its unnamed sister which contains the Chlorophyta and Charophyta/Streptophyta. The land plants ( Embryophytes) have emerged deep in the Charophyte alga a ...

.Silverside, A. J. (2009). Retrieved from http://www.bioref.lastdragon.org/Chlorophyta/''Trebouxia''.html It is a

photosynthetic Photosynthesis is a process used by plants and other organisms to convert light energy into chemical energy that, through cellular respiration, can later be released to fuel the organism's activities. Some of this chemical energy is stored in ...

organism that can exist in almost all habitats found in polar,

tropical The tropics are the regions of Earth surrounding the Equator. They are defined in latitude by the Tropic of Cancer in the Northern Hemisphere at N and the Tropic of Capricorn in the Southern Hemisphere at S. The tropics are also referred to ...

, and

temperate In geography, the temperate climates of Earth occur in the middle latitudes (23.5° to 66.5° N/S of Equator), which span between the tropics and the polar regions of Earth. These zones generally have wider temperature ranges throughout t ...

regions.Erokhina, L. G., Shatilovich, A. V., Kaminskaya, O. P., & Gilichinskii, D. A. (2004). Spectral Properties of the Green Alga ''Trebouxia'', a Phycobiont of Cryptoendolithic Lichens in the Antarctic Dry Valley. Microbiology,73(4), 420-424. doi:10.1023/b:mici.0000036987.18559Lukesova, A., & Frouz, J. (2007). Soil and Freshwater Micro-Algae as a Food Source for Invertebrates in Extreme Environments. Cellular Origin, Life in Extreme Habitats and Astrobiology Algae and Cyanobacteria in Extreme Environments,265-284. doi:10.1007/978-1-4020-6112-7_14Seckbach, J. (2007). Algae and cyanobacteria in extreme environments. Dordrecht: Springer. doi:https://doi.org/10.1007/978-1-4020-6112-7Seckbach, J. (2002). Symbiosis: Mechanisms and model systems. Dordrecht: Kluwer Academic.John, D. M., Whitton, B. A., & Brook, A. J. (2002). The freshwater algal flora of the British Isles: An identification guide to freshwater and terrestrial algae. Cambridge: Cambridge University Press. It can either exist in a symbiotic relationship with fungi in the form of lichen or it can survive independently as a free-living organism alone or in colonies.Bubrick, P., Galun, M., & Frensdorff, A. (1984). Observations On Free-Living ''Trebouxia'' De Puymalyand ''Pseudotrebouxia'' Archibald, And Evidence That Both Symbionts From Xanthoria Parietina (L.) Th. Fr. Can Be Found Free-Living In Nature. New Phytologist,97(3), 455-462. doi:10.1111/j.1469-8137.1984.tb03611.x ''Trebouxia'' is the most common photobiont in extant

lichens A lichen ( , ) is a composite organism that arises from algae or cyanobacteria living among filaments of multiple fungi species in a mutualistic relationship.Honegger, R. (2018). Fossil lichens from the Lower Devonian and their bacterial and fungal epi- and endobionts. In Biodiversity and Ecology of Fungi, Lichens, and Mosses.(Vol. 34, Biosystematics and Ecology Series, pp. 547-563). Vienna: Austrian Academy of Sciences. Retrieved March 2, 2019, from https://www.researchgate.net/publication/326113052_Fossil_lichens_from_the_Lower_Devonian_and_their_bacterial_and_fungal_epi-and_endobionts It is a primary producer of marine, freshwater and terrestrial ecosystems. It uses

carotenoids Carotenoids (), also called tetraterpenoids, are yellow, orange, and red organic pigments that are produced by plants and algae, as well as several bacteria, and fungi. Carotenoids give the characteristic color to pumpkins, carrots, parsnips, co ...

and

chlorophyll Chlorophyll (also chlorophyl) is any of several related green pigments found in cyanobacteria and in the chloroplasts of algae and plants. Its name is derived from the Greek words , ("pale green") and , ("leaf"). Chlorophyll allow plants to ...

a and b to harvest energy from the sun and provide nutrients to various animals and insects. ''Trebouxia'' may have introduced photosynthesis into the terrestrial habitats approximately 450 million years ago.Horwath, W. R. (2017). The Role of the Soil Microbial Biomass in Cycling Nutrients. In T. K. Russel (Author), Microbial Biomass: A Paradigm Shift In Terrestrial Biogeochemistry(pp. 1-348). World Scientific. doi:https://doi.org/10.1142/q0038 It is also a

bioindicator A bioindicator is any species (an indicator species) or group of species whose function, population, or status can reveal the qualitative status of the environment. The most common indicator species are animals. For example, copepods and other sma ...

of habitat disturbances, freshwater quality, air pollution, carbon dioxide concentration, and climate change.Ismail, A., Marzuki, S., Yusof, N. M., Buyong, F., Said, M. M., Sigh, H., & Zulkifli, A. (2017). Epiphytic Terrestrial Algae (''Trebouxia'' sp.) as a Biomarker Using the Free-Air-Carbon Dioxide-Enrichment (FACE) System. Biology,6(4), 19. doi:10.3390/biology6010019Taylor, T. N., Krings, M., & Taylor, E. L. (2015). Lichens. Fossil Fungi,201-220. doi:10.1016/b978-0-12-387731-4.00010-4 Furthermore, its life cycle is complex and much research needs to be done to characterize it more completely.Ahmadjian, V. (1960). Some New and Interesting Species of ''Trebouxia'', a Genus of Lichenized Algae. American Journal of Botany,47(8), 677. doi:10.2307/2439519Friedl, T. (1993). New Aspects of the Reproduction by Autospores in the Lichen Alga ''Trebouxia'' (Microthamniales, Chlorophyta). Archiv Für Protistenkunde,143(1-3), 153-161. doi:10.1016/s0003-9365(11)80284-8Melkonian, M., & Peveling, E. (1987). Zoospore ultrastructure in species of''Trebouxia'' and''Pseudotrebouxia'' (Chlorophyta). Plant Systematics and Evolution,158(2-4), 183-210. doi:10.1007/bf00936344Sanders, W. B. (2005). Observing microscopic phases of lichen life cycles on transparent substrata placed in situ. The Lichenologist,37(05), 373-382. doi:10.1017/s0024282905015070 For decades, the presence of sexual reproduction was unknown.Kroken, S., & Taylor, J. W. (2000). Phylogenetic Species, Reproductive Mode, and Specificity of the Green Alga''Trebouxia''Forming Lichens with the Fungal GenusLetharia. The Bryologist,103(4), 645-660. doi:10.1639/0007-2745(2000)103 645:psrmas.0.co;2 However, recent molecular evidence of recombination and the observation of sexual fusions of

gametes A gamete (; , ultimately ) is a haploid cell that fuses with another haploid cell during fertilization in organisms that reproduce sexually. Gametes are an organism's reproductive cells, also referred to as sex cells. In species that produce t ...

to form zygotes suggest that sexual reproduction occurs. ''Trebouxia'' is a

paraphyletic In taxonomy, a group is paraphyletic if it consists of the group's last common ancestor and most of its descendants, excluding a few monophyletic subgroups. The group is said to be paraphyletic ''with respect to'' the excluded subgroups. In co ...

group.Friedl, T., & Zeltner, C. (1994). Assessing The Relationships Of Some Coccoid Green Lichen Algae And The Microthamniales (Chlorophyta) With 18S Ribosomal Rna Gene Sequence Comparisons1. Journal of Phycology,30(3), 500-506. doi:10.1111/j.0022-3646.1994.00500.x The

horizontal gene transfer Horizontal gene transfer (HGT) or lateral gene transfer (LGT) is the movement of genetic material between unicellular and/or multicellular organisms other than by the ("vertical") transmission of DNA from parent to offspring (reproduction). HG ...

of protein encoding genes occurred between fungi and ''Trebouxia''.Beck, A., Divakar, P. K., Zhang, N., Molina, M. C., & Struwe, L. (2014). Evidence of ancient horizontal gene transfer between fungi and the terrestrial alga ''Trebouxia''. Organisms Diversity & Evolution,15(2), 235-248. doi:10.1007/s13127-014-0199-x There is also evidence of

intron An intron is any nucleotide sequence within a gene that is not expressed or operative in the final RNA product. The word ''intron'' is derived from the term ''intragenic region'', i.e. a region inside a gene."The notion of the cistron .e., gene. ...

among different strains of ''Trebouxia'' in lichen thalli. The presence of

globose A sphere () is a geometrical object that is a three-dimensional analogue to a two-dimensional circle. A sphere is the set of points that are all at the same distance from a given point in three-dimensional space.. That given point is the ...

cells in fossil lichens from the Lower Devonian period (415 million years ago) that look similar to ''Trebouxia'' indicate the significance of ''Trebouxia'' fungal

symbiosis Symbiosis (from Greek , , "living together", from , , "together", and , bíōsis, "living") is any type of a close and long-term biological interaction between two different biological organisms, be it mutualistic, commensalistic, or parasi ...

throughout the history of Earth.

History of knowledge

The genus ''Trebouxia'' was initially described by Puymaly in 1924.Puymaly, A. D. (1924). Le Chlorococcum humicola (Nag.) Rabenh. Revue Algologique, 1, 107-114 The genus name of ''Trebouxia'' is in honour of Octave Treboux (1876–ca. 1940), who was an Estonian botanist (Mykology and Algology),

plant physiologist Plant physiology is a subdiscipline of botany concerned with the functioning, or physiology, of plants. Closely related fields include plant morphology (structure of plants), plant ecology (interactions with the environment), phytochemistry (bio ...

, from the

National University of Kharkiv The Kharkiv University or Karazin University ( uk, Каразінський університет), or officially V. N. Karazin Kharkiv National University ( uk, Харківський національний університет імені ...

and Riga. The genus was divided into two genera ''Trebouxia'' and ''Pseudotrebouxia''.Nash, T. H., III. (1996). Lichen biology. Cambridge;New York, NY, USA;: Cambridge University Press Some recent studies imply that the differences between two groups are invalid and the genus should be divided in different ways such as splitting ''Trebouxia'' into two genera, ''Asterochloris'' (including photobionts of suborder Cladoniinae) and ''Trebouxia'' (including photoboints of suborder Lecanorineae). Trebouxia’s systematic location and taxonomy has been uncertain for decades. Initially, in 1995, the group was placed in the order Pleurastrales Hoek, C. v. d., Mann, D. G., & Jahns, H. M. (1995). Algae: An introduction to phycology. Cambridge;New York;: Cambridge University Press and then in Microthamniales. Later in 2002, it was part of the order Chlorococcales and now it is placed in the order Trebouxiales. It is unknown whether all photobionts described as “trebouxioid” belong to a single genus. Also, it is also unclear how many and which species should be accepted and recognized. Furthermore, in earlier years, classification and nomenclature of species was based on organism’s color, size, growth and shape of colonies, texture, and the lichen it was isolated from. It was believed that each algae species belonged to a specific lichen species. However, in recent years, each ''Trebouxia'' species has been treated independently from lichen species since the same species of ''Trebouxia'' can be associated with many lichens. Now, the classification and nomenclature of species is based on pyrenoid structure, electron dense vesicles, chloroplast and grana shape, vegetative cell size and thickness of cell wall.Casano, L. M., Campo, E. M., García-Breijo, F. J., Reig-Armiñana, J., Gasulla, F., Hoyo, A. D., . . . Barreno, E. (2010). Two ''Trebouxia'' algae with different physiological performances are ever-present in lichen thalli of Ramalina farinacea. Coexistence versus Competition? Environmental Microbiology,13(3), 806-818. doi:10.1111/j.1462-2920.2010.02386.x

Habitat and ecology

''Trebouxia'' is a photosynthetic autotrophic genus that can exist in almost every environmental condition in nature. It can be found in the tropics, Arctic, Antarctic, boreal forest, fresh water, marine, bare rocks, wood debris, tree bark, sandstone, soil, hot and semi-arid deserts.Anderson, O. R. (2014). Microbial Communities Associated with Tree Bark Foliose Lichens: A Perspective on their Microecology. Journal of Eukaryotic Microbiology,61(4), 364-370. doi:10.1111/jeu.12116Rascio, N., & Rocca, N. L. (2013). Biological Nitrogen Fixation. Reference Module in Earth Systems and Environmental Sciences. doi:10.1016/b978-0-12-409548-9.00685-0 Some species can live in extreme conditions such as dry valleys of Antarctica with less than 5% soil moisture or habitats that are rich in iron and metals.Fell, J. W., Scorzetti, G., Connell, L., & Craig, S. (2006). Biodiversity of micro-eukaryotes in Antarctic Dry Valley soils with. Soil Biology and Biochemistry,38(10), 3107-3119. doi:10.1016/j.soilbio.2006.01.014 It can tolerate a wide range of temperatures and prolonged periods of desiccation;.Determeyer-Wiedmann, N., Sadowsky, A., Convey, P., & Ott, S. (2018). Physiological life history strategies of photobionts of lichen species from Antarctic and moderate European habitats in response to stressful conditions. Polar Biology,42(2), 395-405. doi:10.1007/s00300-018-2430-2Sadowsky, A., Mettler-Altmann, T., & Ott, S. (2016). Metabolic response to desiccation stress in strains of green algal photobionts (''Trebouxia'') from two Antarctic lichens of southern habitats. Phycologia,55(6), 703-714. doi:10.2216/15-127.1 Carotenoids such as xanthophyll astaxanthin allow ''Trebouxia'' to tolerate high irradiance.Bidigare, R. R., Ondrusek, M. E., Kennicutt, M. C., Iturriaga, R., Harvey, H. R., Hoham, R. W., & Macko, S. A. (1993). Evidence A Photoprotective For Secondary Carotenoids Of Snow Algae1. Journal of Phycology,29(4), 427-434. doi:10.1111/j.1529-8817.1993.tb00143.x Furthermore, ''Trebouxia'' can exist in its free-living form or in a lichen thallus as a photobiont partner with its fungi mycobiont. The release or escape of alga zoospores from intact lichens is a source of free-living algae colonies or single free-living cells. Moreover, the same ''Trebouxia'' species can be associated with many mycobiont species or many ''Trebouxia'' strains can inhibit single lichen.Catalá, S., Campo, E. M., Barreno, E., García-Breijo, F. J., Reig-Armiñana, J., & Casano, L. M. (2016). Coordinated ultrastructural and phylogenomic analyses shed light on the hidden phycobiont diversity of ''Trebouxia'' microalgae in Ramalina fraxinea. Molecular Phylogenetics and Evolution,94, 765-777. doi:10.1016/j.ympev.2015.10.021 However, the maturation of the lichen could lead to the elimination of all ''Trebouxia'' strains except one. Also, ''Trebouxia'' species are not selective towards their fungal symbionts while fungal species are very selective regarding their algae partners. In areas where algae species are scarce, fungi are less selective and forms a symbiotic relationship with any ''Trebouxia'' species and later on switch to a more suitable algae species. Some ''Trebouxia'' species are highly dependent on their fungal partners and cannot exist as independent organisms.Rikkinen, J. (1995). Whats behind the pretty colours? A study on the photobiology of lichens. Helsinki: Finish Biological SocietyRikkinen, J. (2002). Cyanolichens: An Evolutionary Overview.Dordrecht: Springer. doi:https://doi.org/10.1007/0-306-48005-0_4 Fungi obtain nutrients through self parasitism or selectively harvesting old ''Trebouxia'' cells. ''Trebouxia'', on the other hand, provides 90% of its photosynthetic products to the mycoboint. Pyrenoglobuli (lipid rich stores in the pyrenoid of ''Trebouxia'') are used by the mycoboint for energy and water. ''Trebouxia'' acts as an important primary producer in freshwater, marine, and terrestrial ecosystems. ''Trebouxia'' uses carotenoids and chlorophyll a and b to harvest energy from the sun and synthesize organic compounds that serve as a substantial food source for a wide range of heterotrophs including animals, invertebrates and insects.

Description of the organism

Morphology

''Trebouxia'' is a unicellular spherical green alga that contains a star-like (stellate) or aggregated chloroplast with a single pyrenoid (aggregation of enzymes) at the centre. The size of cells can range from 8- 21 μm in length.Škaloud, P. (2008). ''Trebouxia'' potteri. Retrieved from https://botany.natur.cuni.cz/skaloud/Treb/Trepot.htm ''Trebouxia'' is divided into two groups based on shape of vegetative cells and nature of chromatophore. In the first group, chromatophores are located in the parietal position during the cell division and are deeply incised with irregular, narrow processes that extend to the cell wall and compress against it. The shape of vegetative cells is ellipsoidal in group 1. In group 2, chromatophores are smooth-margined structures located in a central position during the cell division and are not compressed against the cell wall. The vegetative cells are spherical in group 2. All ''Trebouxia'' associated with lichen possess lipid-rich globules in their pyrenoids known as pyrenoglobuli.McCoy, A. G. (1977). Nutritional, morphological, and physiological characteristics of Trentepohlia (I.U. 1227) in axenic culture on defined media. Retrieved from https://ir.library.oregonstate.edu/xmlui/handle/1957/44218 Pyrenoglobuli are used by fungi in the lichen thallus for energy and as a water source. ''Trebouxia'' phycobionts possess different amounts and types of pigments such as chlorophylls and carotenoids in different environmental conditions. For instance, Antarctic ''Trebouxia'' contains low chlorophyll a, high chlorophyll b, and diverse carotenoids compared to Mid-European ''Trebouxia'' phycobionts due to the low-temperature fluorescence spectra in Antarctica.

Reproduction and life cycle

Reproduction in ''Trebouxia'' is mediated by autospores and zoospores. Autospores are non-motile spores that have the same shape as their parent cells. They are produced inside the parent cells. Zoospores are motile spores that are produced inside the lichen thalli and released. They are similar in structure and size in all ''Trebouxia'' taxa. They are 4-6 μm in size and do not possess a cell wall. This allows them to change shape and fit into the fungal network. Later in development, they round up to form walls and become vegetative cells. The zoospores are flattened cells that contain a cup- shaped green chromatophore and two flagella of equal length arising from the basal body and extending beyond the length of body. They contain one contractile vacuole, nucleus, dictyosome, chloroplast, and single mitochondrial reticulum or branched mitochondria linked to microbody. Some species have a stigma (eyespots) which helps orient zoospores towards high light intensity. There are two types of endoplasmic reticulum cisternae. One type connects to two basal bodies and one nucleus. The other type attaches to left and right plasma membrane at cell surfaces. The cell division of ''Trebouxia'' occurs by the cleavage of the chromatophore into two equal halves followed by the pyrenoid division. The pyrenoid can either divide by simple constriction or it can disappear during the division of the chromatophore as observed during zoosporogenesis. In some cells, the nucleus divides before the second division of chromatophore halves whereas in other cells it divides after the second division of the chromatophore by migrating to centre of cell between chromatophore halves. The detail of cell division is understudied and more research needs to be conducted. ''Trebouxia'' has a complex life cycle. The details of the life cycle are not properly understood and more research is required. Reproduction in ''Trebouxia'' can occur by zoospores or autospores. Zoospores are flagellated motile stages within lichens that migrate and settle near fungal spores when liquid water is present.Archibald, P. A. (1975). ''Trebouxia'' de Pulmaly (Chlorophyceae, Chlorococcales) and ''Pseudotrebouxia'' gen. nov. (Chlorophyceae, Chlorosarcinales). Phycologia,14(3), 125-137. doi:10.2216/i0031-8884-14-3-125.1Tschermak-Woess, E. (1989). Developmental studies in trebouxioid algae and taxonomical consequences. Plant Systematics and Evolution,164(1-4), 161-195. doi:10.1007/bf00940436 The clustering of zoospores around fungal spores can lead to the secretion of an attractant that induces zoospores settlement.Ahmadjian, V. (1967). A Guide to the Algae Occurring as Lichen Symbionts: Isolation, Culture, Cultural Physiology, and Identification. Phycologia,6(2-3), 127-160. doi:10.2216/i0031-8884-6-2-127.1 Once the zoospores settle, they change shape and round up to fit into the fungal network. The first cell division after zoospore settlement can either result in the formation of zoosporangium/ autosporagium with 4 to 32 adhering autospore packages (tetrads) or into differentiated vegetative cells. These differentiated vegetative cells are later transformed into zoosporangium/ autosporangia with numerous small autospores, but without adhering packages or tetrad formation. The formation of autospores can occur in two ways. The first way is in which species with permanent aplanosporic (autospore) state arrest the development of zoospores. The division of the chloroplast is accompanied by rounding off and developing a cell wall. In the second way, polygonal- like divisions of the chloroplast form reproductive daughter cells with the cell walls independent of parent cells. The production of aplanospores (autospores) in the second way leads to the development of 16-32 spores in the sporangium. For many years, no sexual structures or observation of sexual reproduction in ''Trebouxia'' were observed.Friedl, T., & Rokitta, C. (1997). Species relationships in the lichen alga ''Trebouxia'' (Chlorophyta, Trebouxiophyceae): molecular phylogenetic analyses of nuclear-encoded large subunit rRNA gene sequences. Symbiosis, Philadelphia, Pa.(USA) However, in recent years, through molecular methods, evidence of recombination and sexual fusions of gamates of the same size suggests the occurrence of sexual reproduction. The zygotes, quite distinct from zoospores, are 6.6 μm in diameter and smooth walled with two round chloroplasts. First, the gametes pair up and fuse with each other, leading to the formation of zygotes. Then, the flagella disappear and the zygote develops in a normal vegetative pattern. Initially, it was thought that the fungus suppresses sexual reproduction in ''Trebouxia'' to inhibit the formation of novel genotypes that could be less suitable for symbiosis. However, it has recently been proposed that ''Trebouxia'' are more likely to reproduce sexually in lichen thallus. Furthermore, gametes from different ''Trebouxia'' species can escape the thallus and fuse to form hybrids or divide asexually to form micro colonies that can later be lichenized by fungi spores. Additional evidence of viable fungi spores and ''Trebouxia'' spores in fecal matter of lichen eating mites provides insight into short and long distance dispersal modes.Meier, F. A., Scherrer, S., & Honegger, R. (2002). Faecal pellets of lichenivorous mites contain viable cells of the lichen-forming ascomycete Xanthoria parietina and its green algal photobiont, ''Trebouxia'' arboricola. Biological Journal of the Linnean Society,76(2), 259-268. doi:10.1046/j.1095-8312.2002.00065.x

Genetics

The

between ''Trebouxia'' and fungi resulted in three

events of protein encoding genes from the fungus genome to the ''Trebouxia'' genome. Within lichen, horizontal gene transfer can also occur among photobionts. The horizontal gene transfer events of

introns An intron is any nucleotide sequence within a gene that is not expressed or operative in the final RNA product. The word ''intron'' is derived from the term ''intragenic region'', i.e. a region inside a gene."The notion of the cistron .e., gene. ...

among different ''Trebouxia'' species have been documented. Many introns can self splice or reverse the splicing reaction or gain motility at DNA or

RNA Ribonucleic acid (RNA) is a polymeric molecule essential in various biological roles in coding, decoding, regulation and expression of genes. RNA and deoxyribonucleic acid ( DNA) are nucleic acids. Along with lipids, proteins, and carbohyd ...

levels that can mediate the transfer process. It is suggested that intron horizontal gene transfer occurs upon the direct cell to cell contact of different ''Trebouxia'' strains in immature lichen thallus. When a lichen thallus matures, only one strain of ''Trebouxia'' remains while others are eliminated. Viruses that infect ''Trebouxia'' and fungi also aid in horizontal gene transfer among different phycobiont species. Furthermore, different techniques have been developed to identify different ''Trebouxia'' strains within lichen thalli. Its rDNA (internal transcribed spacer regions recombinant DNA) sequence comparisons with the aid of

Polymerase chain reaction The polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies (complete or partial) of a specific DNA sample, allowing scientists to take a very small sample of DNA and amplify it (or a part of it) ...

(PCR) are easy and fast ways to identify different ''Trebouxia'' species that inhabit a thallus.

Fossil history

It is known that the most widespread photobiont in extant lichens is ''Trebouxia''. The fossil lichens from the Lower Devonian (415 million years ago) are composed of algae or cyanobacteria and fungi layers. Through the analysis of scanning

electron microscopy An electron microscope is a microscope that uses a beam of accelerated electrons as a source of illumination. As the wavelength of an electron can be up to 100,000 times shorter than that of visible light photons, electron microscopes have a hi ...

, the globose structure of photobionts in ''Chlorolichenomycites salopensis'' lichen species during the Lower Devonian looks similar to ''Trebouxia'' species.

Practical importance

One of the first organisms to colonize terrestrial habitats were lichens. Lichens, along with few other organisms, introduced nitrogen fixation and photosynthesis into terrestrial environments approximately 450 million years ago. They played a significant role in making the harsh terrestrial environment suitable for the colonization of other organisms such as land plants, animals, and insects. Even today lichens make many unsuitable extreme habitats more suitable for species to colonize and survive. Lichens increase the amount of organic matter and organic nitrogen in the soil by producing organic acid that increases rock weathering. Furthermore, lichens are a critical bioindicators of habitat disturbances, freshwater quality, air pollution, carbon dioxide measures, and long term ecological continuity of undisturbed forests. Lichens are also used to date the divergence times of many extinct or extant species. Moreover, lichens are critical in climate change and global warming studies to understand the effects of increasing green house gasses such as carbon dioxide in natural environments. The position of ''Trebouxia'' at the base of the food chain as a primary producer is critical for the maintenance of freshwater, marine, and terrestrial ecosystems. Xanthoria_parietina_(06_03_31)

References

External links

*
''Trebouxia'' (images of British biodiversity)
{{Taxonbar, from=Q653846 Trebouxiophyceae genera Trebouxiophyceae Trebouxiales