Transcription factor II E (TF
IIE) is one of several
general transcription factor
General transcription factors (GTFs), also known as basal transcriptional factors, are a class of protein transcription factors that bind to specific sites ( promoter) on DNA to activate transcription of genetic information from DNA to messenger ...
s that make up the
RNA polymerase II
RNA polymerase II (RNAP II and Pol II) is a multiprotein complex that transcribes DNA into precursors of messenger RNA (mRNA) and most small nuclear RNA (snRNA) and microRNA. It is one of the three RNAP enzymes found in the nucleus of euka ...
preinitiation complex.
It is a tetramer of two alpha and two beta chains and interacts with TAF6/TAFII80, ATF7IP, and varicella-zoster virus IE63 protein.
TF
IIE recruits
TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TF
IIH. Both TF
IIH and TF
IIE are required for promoter clearance by RNA polymerase.
Transcription factor II E is encoded by the
GTF2E1 and
GTF2E2 genes.
TF
IIE is thought to be involved in
DNA melting
Nucleic acid thermodynamics is the study of how temperature affects the nucleic acid structure of double-stranded DNA (dsDNA). The melting temperature (''Tm'') is defined as the temperature at which half of the DNA strands are in the random coil o ...
at the
promoter: it contains a zinc ribbon motif that can bind single stranded DNA.
See also
*
TFIIH
*
TFIIB
*
TFIID
References
External links
*
Molecular genetics
Proteins
Gene expression
Transcription factors
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