Transcription factor TF
IIA is a
nuclear
Nuclear may refer to:
Physics
Relating to the nucleus of the atom:
*Nuclear engineering
*Nuclear physics
*Nuclear power
*Nuclear reactor
*Nuclear weapon
*Nuclear medicine
*Radiation therapy
*Nuclear warfare
Mathematics
*Nuclear space
*Nuclear ...
protein
Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues. Proteins perform a vast array of functions within organisms, including catalysing metabolic reactions, DNA replication, res ...
involved in the
RNA polymerase II
RNA polymerase II (RNAP II and Pol II) is a multiprotein complex that transcribes DNA into precursors of messenger RNA (mRNA) and most small nuclear RNA (snRNA) and microRNA. It is one of the three RNAP enzymes found in the nucleus of euka ...
-dependent
transcription of
DNA.
TF
IIA is one of several general (basal)
transcription factor
In molecular biology, a transcription factor (TF) (or sequence-specific DNA-binding factor) is a protein that controls the rate of transcription of genetic information from DNA to messenger RNA, by binding to a specific DNA sequence. The f ...
s (
GTFs) that are required for all transcription events that use RNA polymerase II. Other GTFs include
TFIID, a complex composed of the
TATA binding protein
The TATA-binding protein (TBP) is a general transcription factor that binds specifically to a DNA sequence called the TATA box. This DNA sequence is found about 30 base pairs upstream of the transcription start site in some eukaryotic gene pr ...
TBP and TBP-associated factors (TAFs), as well as the factors
TFIIB,
TFIIE,
TFIIF, and
TFIIH. Together, these factors are responsible for
promoter recognition and the formation of a transcription
preinitiation complex (PIC) capable of initiating
RNA synthesis from a DNA template.
Functions
TF
IIA interacts with the
TBP subunit of
TFIID and aids in the binding of TBP to
TATA-box containing
promoter DNA.
Interaction of TF
IIA with TBP facilitates formation of and stabilizes the
preinitiation complex. Interaction of TF
IIA with TBP also results in the exclusion of negative (repressive) factors that might otherwise bind to TBP and interfere with PIC formation. TF
IIA also acts as a
coactivator for some transcriptional
activators, assisting with their ability to increase, or activate, transcription. The requirement for TF
IIA ''in vitro'' transcription systems has been variable, and it can be considered either as a GTF and/or a loosely associated TAF-like coactivator. Genetic analysis in
yeast
Yeasts are eukaryotic, single-celled microorganisms classified as members of the fungus kingdom. The first yeast originated hundreds of millions of years ago, and at least 1,500 species are currently recognized. They are estimated to constit ...
has shown that TF
IIA is essential for viability.
Structure
TF
IIA is a heterodimer with two
subunits: one large unprocessed (subunit 1, or alpha/beta; gene name ) and one small (subunit 2, or gamma; gene name ).
It was originally believed to be a heterotrimer of an alpha (p35), a beta (p19) and a gamma subunit (p12). In humans, the sizes of the encoded proteins are approximately 55
kD and 12 kD. Both genes are present in species ranging from humans to yeast, and their protein products interact to form a
complex composed of a
beta barrel
In protein structures, a beta barrel is a beta sheet composed of tandem repeats that twists and coils to form a closed toroidal structure in which the first strand is bonded to the last strand (hydrogen bond). Beta-strands in many beta-barrels are ...
domain
Domain may refer to:
Mathematics
*Domain of a function, the set of input values for which the (total) function is defined
** Domain of definition of a partial function
** Natural domain of a partial function
**Domain of holomorphy of a function
* ...
and an
alpha helical bundle domain. It is the
N-terminal
The N-terminus (also known as the amino-terminus, NH2-terminus, N-terminal end or amine-terminus) is the start of a protein or polypeptide, referring to the free amine group (-NH2) located at the end of a polypeptide. Within a peptide, the ami ...
and
C-terminal
The C-terminus (also known as the carboxyl-terminus, carboxy-terminus, C-terminal tail, C-terminal end, or COOH-terminus) is the end of an amino acid chain (protein or polypeptide), terminated by a free carboxyl group (-COOH). When the protein is ...
regions of the large subunit that participate in interactions with the small subunit. These regions are separated by another domain whose sequence is always present in large subunits from various species but whose size varies and whose sequence is poorly
conserved. A second gene encoding a large TF
IIA subunit has been found in some higher
eukaryote
Eukaryotes () are organisms whose cells have a nucleus. All animals, plants, fungi, and many unicellular organisms, are Eukaryotes. They belong to the group of organisms Eukaryota or Eukarya, which is one of the three domains of life. Bacter ...
s. This gene, ALF/TFIIAtau (gene name ) is expressed only in
oocyte
An oocyte (, ), oöcyte, or ovocyte is a female gametocyte or germ cell involved in reproduction. In other words, it is an immature ovum, or egg cell. An oocyte is produced in a female fetus in the ovary during female gametogenesis. The female ...
s and
spermatocytes, suggesting it has a TF
IIA-like regulatory role for gene expression only in
germ cell
Germ or germs may refer to:
Science
* Germ (microorganism), an informal word for a pathogen
* Germ cell, cell that gives rise to the gametes of an organism that reproduces sexually
* Germ layer, a primary layer of cells that forms during embr ...
s.
References
External links
*
{{Transcription factors, g2
Gene expression
Transcription factors