Turbidimetry (the name being derived from ''
turbidity'') is the process of measuring the loss of intensity of transmitted
light due to the
scattering
Scattering is a term used in physics to describe a wide range of physical processes where moving particles or radiation of some form, such as light or sound, are forced to deviate from a straight trajectory by localized non-uniformities (including ...
effect of particles suspended in it. Light is passed through a filter creating a light of known
wavelength which is then passed through a
cuvette containing a solution. A
photoelectric cell collects the light which passes through the cuvette. A measurement is then given for the amount of absorbed light.
Turbidimetry can be used in
biology to find the
number of cells in a suspension.
Turbidity-is an expression of optical look of a suspension caused by radiation to the scattered and absorbed wavelength. Scattering of light is elastic so both incident and scattered radiation have same wavelength.
A turbidometer measures the amount of radiation that passes through a fluid in forward direction, analogous to absorption spectrphotoometry.
Standard for turbidimetry is prepared by dissolving 5g of hydrazinium (2+) sulfate(N2H4H2SO4) and 50g of hexamethylenetertramine in 1liter of distilled water is defined as 4000 nephlometric Turbidity Unit(NTU)
Application
Determination of water
Clarity of pharma products and drinks
Immunoassay in lab
Turbidimetry offers little advantage than nephelometry in measurement of sensitivity in low level antigen a antibody immunoassay.
Antigen excess and matrix effects are limitations encountered
Immunoturbidimetry
Immunoturbidimetry is an important tool in the broad diagnostic field of clinical chemistry. It is used to determine serum proteins not detectable with classical clinical chemistry methods. Immunoturbidimetry uses the classical antigen-antibody reaction. The antigen-antibody complexes aggregate to form particles that can be optically detected by a photometer.
See also
*
Colorimetry
*
Turbidity
References
Measurement
Chemical tests
Immunologic tests
Scattering
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