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In
genetic engineering Genetic engineering, also called genetic modification or genetic manipulation, is the modification and manipulation of an organism's genes using technology. It is a set of technologies used to change the genetic makeup of cells, including t ...
, transposon tagging is a process where
transposon A transposable element (TE, transposon, or jumping gene) is a nucleic acid sequence in DNA that can change its position within a genome, sometimes creating or reversing mutations and altering the cell's genetic identity and genome size. Transpo ...
s (transposable elements) are amplified inside a biological cell by a tagging technique. Transposon tagging has been used with several
species In biology, a species is the basic unit of classification and a taxonomic rank of an organism, as well as a unit of biodiversity. A species is often defined as the largest group of organisms in which any two individuals of the appropriate s ...
to isolate
gene In biology, the word gene (from , ; "...Wilhelm Johannsen coined the word gene to describe the Mendelian units of heredity..." meaning ''generation'' or ''birth'' or ''gender'') can have several different meanings. The Mendelian gene is a ba ...
s. Even without knowing the nature of the specific genes, the process can still be used.


In plants

By molecular separation of
transposon A transposable element (TE, transposon, or jumping gene) is a nucleic acid sequence in DNA that can change its position within a genome, sometimes creating or reversing mutations and altering the cell's genetic identity and genome size. Transpo ...
s, from a cell nucleus, the
cloning Cloning is the process of producing individual organisms with identical or virtually identical DNA, either by natural or artificial means. In nature, some organisms produce clones through asexual reproduction. In the field of biotechnology, cl ...
is enabled for genes which contain the transposons. By using transposon tagging, researchers have been able to add genetic elements from
maize Maize ( ; ''Zea mays'' subsp. ''mays'', from es, maĆ­z after tnq, mahiz), also known as corn (North American and Australian English), is a cereal grain first domesticated by indigenous peoples in southern Mexico about 10,000 years ago. Th ...
(corn) and
Antirrhinum ''Antirrhinum'' is a genus of plants commonly known as dragon flowers, snapdragons and dog flower because of the flowers' fancied resemblance to the face of a dragon that opens and closes its mouth when laterally squeezed. They are native t ...
into some other species (such as
tobacco Tobacco is the common name of several plants in the genus '' Nicotiana'' of the family Solanaceae, and the general term for any product prepared from the cured leaves of these plants. More than 70 species of tobacco are known, but the ...
, aspen and others). A gene responsible for a particular
phenotype In genetics, the phenotype () is the set of observable characteristics or traits of an organism. The term covers the organism's morphology or physical form and structure, its developmental processes, its biochemical and physiological proper ...
can be cloned within a given species, when movement is accompanied by the presence of a mutant phenotype.


References


Further reading


''Transposon Tagging''
North Dakota State University *A.M. Settles
''Transposon Tagging and Reverse Genetics''
University of Florida Biochemistry detection methods Cloning Genetic engineering Genetics techniques {{genetics-stub