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Tibicos, or water kefir, is a traditional
fermented drink This is a list of fermented foods, which are foods produced or preserved by the action of microorganisms. In this context, Fermentation in food processing, fermentation typically refers to the fermentation of sugar to ethanol, alcohol using yeas ...
made with water and a
symbiotic Symbiosis (from Greek , , "living together", from , , "together", and , bíōsis, "living") is any type of a close and long-term biological interaction between two different biological organisms, be it mutualistic, commensalistic, or parasit ...
culture Culture () is an umbrella term which encompasses the social behavior, institutions, and norms found in human societies, as well as the knowledge, beliefs, arts, laws, customs, capabilities, and habits of the individuals in these groups.Tyl ...
of
bacteria Bacteria (; singular: bacterium) are ubiquitous, mostly free-living organisms often consisting of one biological cell. They constitute a large domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria were among ...
and
yeast Yeasts are eukaryotic, single-celled microorganisms classified as members of the fungus kingdom. The first yeast originated hundreds of millions of years ago, and at least 1,500 species are currently recognized. They are estimated to constitut ...
s (
SCOBY Symbiotic culture of bacteria and yeast (SCOBY) is a culinary symbiotic fermentation culture ( starter) consisting of lactic acid bacteria (LAB), acetic acid bacteria (AAB), and yeast which arises in the preparation of sour foods and beverages s ...
) held in a
polysaccharide Polysaccharides (), or polycarbohydrates, are the most abundant carbohydrates found in food. They are long chain polymeric carbohydrates composed of monosaccharide units bound together by glycosidic linkages. This carbohydrate can react with wa ...
biofilm A biofilm comprises any syntrophic consortium of microorganisms in which cells stick to each other and often also to a surface. These adherent cells become embedded within a slimy extracellular matrix that is composed of extracellular ...
matrix Matrix most commonly refers to: * ''The Matrix'' (franchise), an American media franchise ** ''The Matrix'', a 1999 science-fiction action film ** "The Matrix", a fictional setting, a virtual reality environment, within ''The Matrix'' (franchis ...
created by the bacteria. It is sometimes consumed as an alternative to milk-based
probiotic Probiotics are live microorganisms promoted with claims that they provide health benefits when consumed, generally by improving or restoring the gut microbiota. Probiotics are considered generally safe to consume, but may cause bacteria-host i ...
drinks or tea-cultured products such as
kombucha Kombucha (also tea mushroom, tea fungus, or Manchurian mushroom when referring to the culture; Latin name ''Medusomyces gisevii'') is a fermented, lightly effervescent, sweetened black tea drink commonly consumed for its purported health ben ...
. Water kefir is typically made as a probiotic homebrew beverage. The finished product, if bottled, will produce a carbonated beverage.


Cultures

Tibicos cultures are found around the world, with no two being exactly the same; but typical tibicos have a mix of ''
Lactobacillus ''Lactobacillus'' is a genus of Gram-positive, aerotolerant anaerobes or microaerophilic, rod-shaped, non-spore-forming bacteria. Until 2020, the genus ''Lactobacillus'' comprised over 260 phylogenetically, ecologically, and metabolically diver ...
'', ''
Streptococcus ''Streptococcus'' is a genus of gram-positive ' (plural ) or spherical bacteria that belongs to the family Streptococcaceae, within the order Lactobacillales (lactic acid bacteria), in the phylum Bacillota. Cell division in streptococci occurs ...
'', ''
Pediococcus ''Pediococcus'' is a genus of gram-positive lactic acid bacteria, placed within the family of Lactobacillaceae. They usually occur in pairs or tetrads, and divide along two planes of symmetry, as do the other lactic acid cocci genera ''Aerococcu ...
'' and ''
Leuconostoc ''Leuconostoc'' is a genus of gram-positive bacteria, placed within the family of Lactobacillaceae. They are generally ovoid cocci often forming chains. ''Leuconostoc'' spp. are intrinsically resistant to vancomycin and are catalase-negative (wh ...
'' bacteria, with yeasts from ''
Saccharomyces ''Saccharomyces'' is a genus of fungi that includes many species of yeasts. ''Saccharomyces'' is from Greek σάκχαρον (sugar) and μύκης (fungus) and means ''sugar fungus''. Many members of this genus are considered very important in f ...
'', '' Candida'', ''
Kloeckera ''Hanseniaspora'' is a genus of yeasts. The name ''Kloeckera'' is applied to its anamorph form. They are typically apiculate (lemon-shaped) in shape and often found in grape musts pre-fermentation. The genus name ''Hanseniaspora'' honours Emil ...
'' and possibly others. ''
Lactobacillus brevis ''Levilactobacillus brevis'' is a gram-positive, rod shaped species of lactic acid bacteria which is heterofermentative, creating CO2, lactic acid and acetic acid or ethanol during fermentation. ''L. brevis'' is the type species of the genus ''Le ...
'' bacteria has been identified as the species responsible for the production of the
dextran Dextran is a complex branched glucan (polysaccharide derived from the condensation of glucose), originally derived from wine. IUPAC defines dextrans as "Branched poly-α-d-glucosides of microbial origin having glycosidic bonds predominantly C-1 ...
polysaccharide Polysaccharides (), or polycarbohydrates, are the most abundant carbohydrates found in food. They are long chain polymeric carbohydrates composed of monosaccharide units bound together by glycosidic linkages. This carbohydrate can react with wa ...
that forms the "grains." As with milk kefir "grains", the
microbes A microorganism, or microbe,, ''mikros'', "small") and ''organism'' from the el, ὀργανισμός, ''organismós'', "organism"). It is usually written as a single word but is sometimes hyphenated (''micro-organism''), especially in olde ...
present in tibicos act in
symbiosis Symbiosis (from Greek , , "living together", from , , "together", and , bíōsis, "living") is any type of a close and long-term biological interaction between two different biological organisms, be it mutualistic, commensalistic, or parasit ...
to maintain a stable culture. Tibicos can do this in many different sugary liquids, feeding off the sugar to produce
lactic acid Lactic acid is an organic acid. It has a molecular formula . It is white in the solid state and it is miscible with water. When in the dissolved state, it forms a colorless solution. Production includes both artificial synthesis as well as natu ...
, alcohol (
ethanol Ethanol (abbr. EtOH; also called ethyl alcohol, grain alcohol, drinking alcohol, or simply alcohol) is an organic compound. It is an Alcohol (chemistry), alcohol with the chemical formula . Its formula can be also written as or (an ethyl ...
), and
carbon dioxide Carbon dioxide (chemical formula ) is a chemical compound made up of molecules that each have one carbon atom covalently double bonded to two oxygen atoms. It is found in the gas state at room temperature. In the air, carbon dioxide is transpar ...
gas, which
carbonates A carbonate is a salt of carbonic acid (H2CO3), characterized by the presence of the carbonate ion, a polyatomic ion with the formula . The word ''carbonate'' may also refer to a carbonate ester, an organic compound containing the carbonate g ...
the drink.


Origin

The origin of tibicos grains is unknown. Tibicos grains form as hard granules on the pads of the ''
Opuntia ''Opuntia'', commonly called prickly pear or pear cactus, is a genus of flowering plants in the cactus family Cactaceae. Prickly pears are also known as ''tuna'' (fruit), ''sabra'', ''nopal'' (paddle, plural ''nopales'') from the Nahuatl word f ...
'' cactus found in Mexico. These granules then could be reconstituted in a sugar-water solution for propagating the tibicos grains. Another study found a similar tibicos culture made from bacteria cultured from known stocks with similar properties. Tibicos are also known as tibi, water kefir grains, sugar kefir grains, Japanese water crystals and California bees, and in older literature as ''bébées'', African bees, Australian bees, ginger bees, vinegar bees, bees, Japanese beer seeds, beer seeds, beer plant, ale nuts, eternity grains, and
Balm of Gilead Balm of Gilead was a rare perfume used medicinally, that was mentioned in the Hebrew Bible, and named for the region of Gilead, where it was produced. The expression stems from William Tyndale's language in the King James Bible of 1611, and has c ...
. Pidoux in 1898 also identified the sugary kefir grains with the ginger beer plant. Different ingredients or hygienic conditions might also change the bacteriological composition possibly leading to the different names found in scientific literature. Tibicos are used to brew a variety of
tepache Tepache is a fermented beverage made from the peel and the rind of pineapples, and is sweetened either with ''piloncillo'' or brown sugar, seasoned with powdered cinnamon, and served cold. Though tepache is fermented for several days, the resul ...
known as ''tepache de tibicos''. The '' ginger beer plant'' is also a form of tibicos. Kebler attests that they were used in Kentucky circa 1859 to brew a "home drink" and were referred to as "Japanese beer seeds."


Preparation

The basic preparation method is for tibicos to be added to a sugary liquid and
fermented Fermentation is a metabolic process that produces chemical changes in organic substrates through the action of enzymes. In biochemistry, it is narrowly defined as the extraction of energy from carbohydrates in the absence of oxygen. In food ...
24 to 48 hours. The water is kept at a room temperature range of . If the temperature is towards the upper end of this range, the fermentation period is shortened. A typical recipe might contain the tibicos culture, a citrus fruit, and water. Some ingredients will inhibit fermentation, such as
chlorine Chlorine is a chemical element with the Symbol (chemistry), symbol Cl and atomic number 17. The second-lightest of the halogens, it appears between fluorine and bromine in the periodic table and its properties are mostly intermediate betwee ...
in tap water or preservatives in dried fruit (sulfites). The fruits used are changed and mixed to create different flavors. Additional precautions are taken to keep the cultures healthy. The use of reactive metals such as aluminium, copper, or zinc are minimized, since the acidity of the solution will draw these metals out, damaging the culture. Instead, plastic, lead-free ceramic, or glass containers are commonly used. It is recommended to culture grains in a glass jar and use clean plastic or wooden utensils when handling the grains.


See also

*
Kefir Kefir ( ; also spelled as kephir or kefier; ; ; ) is a fermented milk drink similar to a thin yogurt or ayran that is made from kefir grains, a specific type of mesophilic symbiotic culture. The drink originated in the North Caucasus, in pa ...
*
Kombucha Kombucha (also tea mushroom, tea fungus, or Manchurian mushroom when referring to the culture; Latin name ''Medusomyces gisevii'') is a fermented, lightly effervescent, sweetened black tea drink commonly consumed for its purported health ben ...


External links


Water Kefir: A Review of its Microbiological Profile, Antioxidant Potential and Sensory Quality

An update on water kefir: Microbiology, composition and productionDevelopment of new non-dairy beverages from Mediterranean fruit juices fermented with water kefir microorganisms

Characterization of kefir-like beverages produced from vegetable juiceFunctional Exploitation of Carob, Oat Flour, and Whey Permeate as Substrates for a Novel Kefir-Like Fermented Beverage: An Optimized FormulationDevelopment, Characterization, and Bioactivity of Non-Dairy Kefir-Like Fermented Beverage Based on Flaxseed Oil Cake

5 Scientific Benefits of Water KefirAntioxidant potency of water kefirWater Kefir and Derived Pasteurized Beverages Modulate Gut Microbiota, Intestinal Permeability and Cytokine Production In VitroStudy of anti-inflammatory activity of Tibetan mushroom, a symbiotic culture of bacteria and fungi encapsulated into a polysaccharide matrixProbiotic Potential of Lactobacillus paracasei CT12 Isolated from Water Kefir Grains (Tibicos)Water kefir: Factors affecting grain growth and health-promoting properties of the fermented beverageAnti-Inflammatory and Cicatrizing Activities of a Carbohydrate Fraction Isolated from Sugary KefirAntimicrobial Activity of Broth Fermented with Kefir GrainsMicrobial Species Diversity, Community Dynamics, and Metabolite Kinetics of Water Kefir FermentationThe Buffer Capacity and Calcium Concentration of Water Influence the Microbial Species Diversity, Grain Growth, and Metabolite Production During Water Kefir FermentationPollution Abatement of Heavy Metals in Different Conditions by Water Kefir Grains as a Protective Tool against ToxicityQuantitative insights on the interaction between metal ions and water kefir grains: kinetics studies and EPR investigations


References

{{Reflist, 30em Fermented foods Fermented drinks