tRNA (cytidine³⁴-2'-O)-methyltransferase (, ''yibK (gene)'', ''methyltransferase yibK'', ''TrmL'', ''tRNA methyltransferase L'', ''tRNA (cytidine^34/5-carboxymethylaminomethyluridine³⁴-2'-O)-methyltransferase'') is an

enzyme Enzymes () are proteins that act as biological catalysts by accelerating chemical reactions. The molecules upon which enzymes may act are called substrates, and the enzyme converts the substrates into different molecules known as products ...

with

systematic name A systematic name is a name given in a systematic way to one unique group, organism, object or chemical substance, out of a specific population or collection. Systematic names are usually part of a nomenclature. A semisystematic name or semitrivial ...

''S-adenosyl-L-methionine:tRNA (cytidine^34/5-carboxymethylaminomethyluridine³⁴-2'-O)-methyltransferase''. This enzyme catalyses the following

chemical reaction A chemical reaction is a process that leads to the IUPAC nomenclature for organic transformations, chemical transformation of one set of chemical substances to another. Classically, chemical reactions encompass changes that only involve the pos ...

: (1)

S-adenosyl-L-methionine ''S''-Adenosyl methionine (SAM), also known under the commercial names of SAMe, SAM-e, or AdoMet, is a common cosubstrate involved in methyl group transfers, transsulfuration, and aminopropylation. Although these anabolic reactions occur through ...

cytidine Cytidine (symbol C or Cyd) is a nucleoside molecule that is formed when cytosine is attached to a ribose ring (also known as a ribofuranose) via a β-N1- glycosidic bond. Cytidine is a component of RNA. It is a white water-soluble solid. which ...

³⁴ in

tRNA Transfer RNA (abbreviated tRNA and formerly referred to as sRNA, for soluble RNA) is an adaptor molecule composed of RNA, typically 76 to 90 nucleotides in length (in eukaryotes), that serves as the physical link between the mRNA and the amino ...

\rightleftharpoons

S-adenosyl-L-homocysteine + 2'-O-methylcytidine³⁴ in tRNA : (2) S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine³⁴ in tRNALeu

\rightleftharpoons

S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine³⁴ in tRNALeu The enzyme from ''

Escherichia coli ''Escherichia coli'' (),Wells, J. C. (2000) Longman Pronunciation Dictionary. Harlow ngland Pearson Education Ltd. also known as ''E. coli'' (), is a Gram-negative, facultative anaerobic, rod-shaped, coliform bacterium of the genus '' Esc ...

'' catalyses the 2'-O-methylation of cytidine or 5-carboxymethylaminomethyluridine at the wobble position at

nucleotide Nucleotides are organic molecules consisting of a nucleoside and a phosphate. They serve as monomeric units of the nucleic acid polymers – deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), both of which are essential biomolecule ...

34 in tRNALeuCmAA and tRNALeucmnm5UmAA.

References

External links

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