Midline-2 is a

protein Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues. Proteins perform a vast array of functions within organisms, including catalysing metabolic reactions, DNA replication, respo ...

that in humans is encoded by the ''MID2''

gene In biology, the word gene (from , ; "...Wilhelm Johannsen coined the word gene to describe the Mendelian units of heredity..." meaning ''generation'' or ''birth'' or ''gender'') can have several different meanings. The Mendelian gene is a ba ...

Function

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Its function has not been identified. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Recent reports indicate the involvement of MID2 in cytokinesis .MID2 (TRIM1) ubiquitinates

Sperm-associated antigen 5 Sperm-associated antigen 5 is a protein that in humans is encoded by the ''SPAG5'' gene In biology, the word gene (from , ; "...Wilhelm Johannsen coined the word gene to describe the Mendelian units of heredity..." meaning ''generation'' o ...

(Astrin) on K409, further promoting its degradation and proper cytokinesis. In contrary, depletion of MID2 (TRIM1) stabilizes

(Astrin) whose inappropriate accumulation at the midbody triggers cytokinetic arrest, multinucleated cells, and cell death.

Interactions

MID2 has been shown to

interact Advocates for Informed Choice, dba interACT or interACT Advocates for Intersex Youth, is a 501(c)(3) nonprofit organization using innovative strategies to advocate for the legal and human rights of children with intersex traits. The organizati ...

with

MID1 MID1 is a protein that belongs to the Tripartite motif family (TRIM) and is also known as TRIM18. The ''MID1'' gene is located on the short arm of the X chromosome and loss-of-function mutations in this gene are causative of the X-linked form of a ...

. MID2 (TRIM1) interacts with

Leucine-rich repeat kinase 2 Leucine-rich repeat kinase 2 (LRRK2), also known as dardarin (from the Basque word "dardara" which means trembling) and PARK8 (from early identified association with Parkinson's disease), is a large, multifunctional kinase enzyme that in humans is ...

(LRRK2), which is often subject to missense mutations in familial Parkinson's disease (PD). MID2 (TRIM1) specifically binds to the flexible regulatory loop of LRRK2_853–981. MID2 (TRIM1) recruits

LRRK2 Leucine-rich repeat kinase 2 (LRRK2), also known as dardarin (from the Basque word "dardara" which means trembling) and PARK8 (from early identified association with Parkinson's disease), is a large, multifunctional kinase enzyme that in humans i ...

to the microtubule cytoskeleton where MID2 (TRIM1) ubiquitinates LRRK2 targeting it for proteasomal degradation.

Function

Interactions

References

Further reading