THP-1 is a human monocytic cell line derived from an

acute monocytic leukemia Acute monocytic leukemia (AMoL, or AML-M5) is a type of acute myeloid leukemia. In AML-M5 >80% of the leukemic cells are of monocytic lineage. This cancer is characterized by a dominance of monocytes in the bone marrow. There is an overproduction o ...

patient. It is used to test

leukemia Leukemia ( also spelled leukaemia and pronounced ) is a group of blood cancers that usually begin in the bone marrow and result in high numbers of abnormal blood cells. These blood cells are not fully developed and are called ''blasts'' or ...

cell lines in immunocytochemical analysis of protein-protein interactions, and immunohistochemistry.

Characteristics

Although THP-1 cells are of the same lineage, mutations can cause differences as the progeny proliferates. In general, THP-1 cells exhibit a large, round, single-cell morphology. The cells were derived from the peripheral blood of a 1-year-old human male with acute monocytic leukemia. Some of their characteristics are: * Expression of Fc receptor and C3b receptors while lacking surface and cytoplasmic immunoglobulins. * Production of IL-1. * Positive detection of alpha-naphthyl butyrate esterase and

lysozyme Lysozyme (EC 3.2.1.17, muramidase, ''N''-acetylmuramide glycanhydrolase; systematic name peptidoglycan ''N''-acetylmuramoylhydrolase) is an antimicrobial enzyme produced by animals that forms part of the innate immune system. It is a glycoside ...

s *

Phagocytic Phagocytosis () is the process by which a cell uses its plasma membrane to engulf a large particle (≥ 0.5 μm), giving rise to an internal compartment called the phagosome. It is one type of endocytosis. A cell that performs phagocytosis is c ...

physiology (both for latex beads and sensitized erythrocytes). * Restoration of the response of purified

T lymphocytes A T cell is a type of lymphocyte. T cells are one of the important white blood cells of the immune system and play a central role in the adaptive immune response. T cells can be distinguished from other lymphocytes by the presence of a T-cell rec ...

to Con A. * Increased CO₂ production on phagocytosis and differentiation into macrophage-like cells * Polarization into the M1 phenotype by incubation with IFN-γ and LPS, or to the M2 phenotype by incubation with interleukin 4 and interleukin 13 * Differentiation into immature dendritic cells, using recombinant human interleukin 4 (rhIL-4) and recombinant human granulocyte macrophage colony-stimulating factor (rhGM-CSF), and mature dendritic cells using rhIL-4, rhGM-CSF, recombinant human tumour necrotic factor α (rhTNF-α) and Ionomycin. * The

HLA type The human leukocyte antigen (HLA) system or complex is a complex of genes on chromosome 6 in humans which encode cell-surface proteins responsible for the regulation of the immune system. The HLA system is also known as the human version of th ...

for THP-1 is HLA-A*02:01; A*24:02; B*15:11; B*35:01; C*03:03; DRB1*01:01; DRB1*15:01; DQB1*05:01; DQB1*06:02; DPB1*02:01; DPB1*04:02 (in the German Collection of Microorganisms and Cell Cultures (DSMZ) cell bank). This HLA type can change depending on the reference biorepository, due to loss of heterozygosity in multiple chromosomal regions, as THP-1 from the American Type Culture Collection (ATCC) do not express the HLA-A*24:02 and B*35:01 alleles.

Growth Information

THP-1 can provide continuous culture when grown in suspension; RPMI 1640 + 10% FBS + 2mM L-Glutamine. The average doubling time is 19 to 50 hours. 1 mM sodium pyruvate, penicillin (100 units/ml) and streptomycin (100 μg/ml) are also commonly added to inhibit bacterial contamination. Cultures should be maintained at cell densities in the range 2-9x10⁵ cells/ml at 37 °C, 5% CO₂. Cells are non-adherent.

Hazards

THP-1 cells are of human origin, and no evidence has been found for the presence of infectious viruses or toxic products. The ATCC Biosafety recommendation is level 1.

Research applications

THP-1 cells are used as ''

in vitro ''In vitro'' (meaning in glass, or ''in the glass'') studies are performed with microorganisms, cells, or biological molecules outside their normal biological context. Colloquially called " test-tube experiments", these studies in biology ...

'' cancer cell models, as well as a models to study the monocyte-macrophage differentiation process, and as a model to examine some macrophage-related physiological processes, for example the macrophage cholesterol efflux.

References

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External links

Cellosaurus entry for THP-1
Hematology Human cell lines