TDBzcholine is a
diazirine analog of
acetylcholine
Acetylcholine (ACh) is an organic chemical that functions in the brain and body of many types of animals (including humans) as a neurotransmitter. Its name is derived from its chemical structure: it is an ester of acetic acid and choline. Part ...
that can be used to label the
nicotinic acetylcholine receptor.
Mechanism of action
TDBzcholine is able to bind to the nicotinic acetylcholine receptor. Once TDBzcholine is bound to the receptor, TDBzcholine can be activated by exposing the sample to
UV light. This led to formation of a highly reactive
carbene
In organic chemistry, a carbene is a molecule containing a neutral carbon atom with a valence of two and two unshared valence electrons. The general formula is or where the R represents substituents or hydrogen atoms.
The term "carbene" ma ...
radical that can react with amino acid residues in the receptor and become covalently bound to the receptor.
See also
*
Photoaffinity labeling
References
Diazirines
Choline esters
Benzoate esters
Trifluoromethyl compounds
Nicotinic acetylcholine receptors
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