Karyotype of the T47D breast cancer cell line

T-47D is a human breast cancer

cell line An immortalised cell line is a population of cells from a multicellular organism which would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division. The cell ...

commonly used in biomedical research involving the hormonal expression of cancer cells.

Characteristics

The cells were derived from the pleural effusion of a

ductal carcinoma Ductal carcinoma is a type of tumor that primarily presents in the ducts of a gland. Types include: * Mammary **Ductal carcinoma in situ **Invasive ductal carcinoma * Pancreatic ductal carcinoma Pancreatic cancer arises when cells in the ...

found in the mammary gland of an elderly human patient. T-47D cells are distinct from other human breast cancer cells in that their

progesterone receptors Progesterone (P4) is an endogenous steroid and progestogen sex hormone involved in the menstrual cycle, pregnancy, and embryogenesis of humans and other species. It belongs to a group of steroid hormones called the progestogens and is the major ...

are not regulated by estradiol, a hormone that is abundant within the cells themselves.

Usage

T-47D cells have been employed in studies of the effects of

progesterone Progesterone (P4) is an endogenous steroid and progestogen sex hormone involved in the menstrual cycle, pregnancy, and embryogenesis of humans and other species. It belongs to a group of steroid hormones called the progestogens and is the m ...

on breast cancer and the corresponding

transcriptional Transcription is the process of copying a segment of DNA into RNA. The segments of DNA transcribed into RNA molecules that can encode proteins are said to produce messenger RNA (mRNA). Other segments of DNA are copied into RNA molecules calle ...

regulation caused by introduced drugs. T-47D cells can be implanted into immunodeficient mice, forming

xenografts Xenotransplantation (''xenos-'' from the Greek meaning "foreign" or strange), or heterologous transplant, is the transplantation of living cells, tissues or organs from one species to another. Such cells, tissues or organs are called xenograft ...

that can undergo drug testing with corresponding receptor status and

angiogenesis Angiogenesis is the physiological process through which new blood vessels form from pre-existing vessels, formed in the earlier stage of vasculogenesis. Angiogenesis continues the growth of the vasculature by processes of sprouting and splitting ...

changes. The cells have been noted to be extremely resistant to estrogens and antiestrogens, possibly due to inhibition by 5-bromodeoxyuridine and sodium butyrate.

References

Human cell lines

External links

Cellosaurus entry for T-47D
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