Stable-isotope probing (SIP) is a technique in microbial ecology for tracing uptake of

nutrient A nutrient is a substance used by an organism to survive, grow, and reproduce. The requirement for dietary nutrient intake applies to animals, plants, fungi, and protists. Nutrients can be incorporated into cells for metabolic purposes or excret ...

s in

biogeochemical cycling A biogeochemical cycle (or more generally a cycle of matter) is the pathway by which a chemical substance cycles (is turned over or moves through) the biotic and the abiotic compartments of Earth. The biotic compartment is the biosphere and the ...

by microorganisms. A substrate is enriched with a heavier stable isotope that is consumed by the organisms to be studied. Biomarkers with the heavier isotopes incorporated into them can be separated from biomarkers containing the more naturally abundant lighter isotope by isopycnic centrifugation. For example, ¹³ CO₂ can be used to find out which organisms are actively photosynthesizing or consuming new photosynthate. As the biomarker, DNA with ¹³C is then separated from DNA with ¹²C by centrifugation.

Sequencing In genetics and biochemistry, sequencing means to determine the primary structure (sometimes incorrectly called the primary sequence) of an unbranched biopolymer. Sequencing results in a symbolic linear depiction known as a sequence which succ ...

the DNA identifies which organisms were consuming existing

carbohydrates In organic chemistry, a carbohydrate () is a biomolecule consisting of carbon (C), hydrogen (H) and oxygen (O) atoms, usually with a hydrogen–oxygen atom ratio of 2:1 (as in water) and thus with the empirical formula (where ''m'' may or may ...

and which were using carbohydrates more recently produced from photosynthesis. SIP with ¹⁸O-labeled water can be used to find out which organisms are actively growing, because oxygen from water is incorporated into DNA (and RNA) during synthesis. When DNA is the biomarker, SIP can be performed using isotopically labeled C, H, O, or N, though ¹³C is used most often. The density shift is proportional to the change in density in the DNA, which depends on the difference in mass between the rare and common isotopes for a given element, and on the abundance of elements in the DNA. For example, the difference in mass between ¹⁸O and ¹⁶O (two atomic mass units) is twice that between ¹³C and ¹²C (one atomic mass unit), so incorporation of ¹⁸O into DNA will cause a larger per atom density shift than will incorporation of ¹³C. Conversely, DNA contains nearly twice as many carbon atoms (11.25 per base, on average) as oxygen atoms (6 per base), so at equivalent labeling (e.g., 50 atom percent ¹³C or ¹⁸O), DNA labeled with ¹⁸O will be only slightly more dense than DNA fully labeled with ¹³C. Similarly, nitrogen is less abundant in DNA (3.75 atoms per base, on average), so a weaker DNA buoyant density shift is observed with ¹⁵N- versus ¹³C-labeled or ¹⁸O-labeled substrates. Larger buoyant density shifts are observed when multiple isotope tracers are used. Because density shifts as a predictable function of the change in mass caused by isotope assimilation, stable isotope probing can be modeled to estimate the amount of isotope incorporation, an approach called quantitative stable isotope probing (qSIP), which has been applied to microbial communities in soils, marine sediments, and decomposing leaves to compare rates of growth and substrate assimilation among different microbial taxa.

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