Somatic Cell Count
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A somatic cell count (SCC) is a
cell count Cell counting is any of various methods for the counting or similar quantification of cells in the life sciences, including medical diagnosis and treatment. It is an important subset of cytometry, with applications in research and clinical prac ...
of
somatic cell A somatic cell (from Ancient Greek σῶμα ''sôma'', meaning "body"), or vegetal cell, is any biological cell forming the body of a multicellular organism other than a gamete, germ cell, gametocyte or undifferentiated stem cell. Such cells compo ...
s in a fluid
specimen Specimen may refer to: Science and technology * Sample (material), a limited quantity of something which is intended to be similar to and represent a larger amount * Biological specimen or biospecimen, an organic specimen held by a biorepository ...
, usually
milk Milk is a white liquid food produced by the mammary glands of mammals. It is the primary source of nutrition for young mammals (including breastfed human infants) before they are able to digestion, digest solid food. Immune factors and immune ...
. In
dairy A dairy is a business enterprise established for the harvesting or processing (or both) of animal milk – mostly from cows or buffaloes, but also from goats, sheep, horses, or camels – for human consumption. A dairy is typically located on ...
ing, the SCC is an indicator of the
quality Quality may refer to: Concepts *Quality (business), the ''non-inferiority'' or ''superiority'' of something *Quality (philosophy), an attribute or a property *Quality (physics), in response theory *Energy quality, used in various science discipli ...
of milk—specifically, its low likeliness to contain harmful
bacteria Bacteria (; singular: bacterium) are ubiquitous, mostly free-living organisms often consisting of one biological cell. They constitute a large domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria were among ...
, and thus its high
food safety Food safety (or food hygiene) is used as a scientific method/discipline describing handling, preparation, and storage of food in ways that prevent food-borne illness. The occurrence of two or more cases of a similar illness resulting from t ...
.
White blood cell White blood cells, also called leukocytes or leucocytes, are the cell (biology), cells of the immune system that are involved in protecting the body against both infectious disease and foreign invaders. All white blood cells are produced and de ...
s (leukocytes) constitute the majority of somatic cells in question. The number of
somatic cell A somatic cell (from Ancient Greek σῶμα ''sôma'', meaning "body"), or vegetal cell, is any biological cell forming the body of a multicellular organism other than a gamete, germ cell, gametocyte or undifferentiated stem cell. Such cells compo ...
s increases in response to
pathogen In biology, a pathogen ( el, πάθος, "suffering", "passion" and , "producer of") in the oldest and broadest sense, is any organism or agent that can produce disease. A pathogen may also be referred to as an infectious agent, or simply a germ ...
ic bacteria like ''
Staphylococcus aureus ''Staphylococcus aureus'' is a Gram-positive spherically shaped bacterium, a member of the Bacillota, and is a usual member of the microbiota of the body, frequently found in the upper respiratory tract and on the skin. It is often positive ...
'', a cause of
mastitis Mastitis is inflammation of the breast or udder, usually associated with breastfeeding. Symptoms typically include local pain and redness. There is often an associated fever and general soreness. Onset is typically fairly rapid and usually occurs ...
. The SCC is quantified as cells per
milliliter The litre (international spelling) or liter (American English spelling) (SI symbols L and l, other symbol used: ℓ) is a metric unit of volume. It is equal to 1 cubic decimetre (dm3), 1000 cubic centimetres (cm3) or 0.001 cubic metre (m3). ...
. General agreement rests on a
reference range In medicine and health-related fields, a reference range or reference interval is the range or the interval of values that is deemed normal for a physiological measurement in healthy persons (for example, the amount of creatinine in the blood, or ...
of less than 100,000 cells/mL for uninfected
cow Cattle (''Bos taurus'') are large, domesticated, cloven-hooved, herbivores. They are a prominent modern member of the subfamily Bovinae and the most widespread species of the genus ''Bos''. Adult females are referred to as cows and adult ma ...
s and greater than 250,000 for cows infected with significant pathogen levels. Several tests like the PortaSCC milk test and The
California mastitis test The California mastitis test (CMT) is a simple cow-side indicator of the somatic cell count of milk. It operates by disrupting the cell membrane of any cells present in the milk sample, allowing the DNA in those cells to react with the test reagen ...
provide a cow-side measure of somatic cell count. The somatic cell count in the milk also increases after calving when
colostrum Colostrum, also known as beestings or first milk, is the first form of milk produced by the mammary glands of mammals (including humans) immediately following delivery of the newborn. Colostrum powder is rich in high protein and low in sugar and ...
is produced.


Bacteria plate count

The methods of determining Grade A milk quality are well established, and are based on the somatic cell count and the ''bacteria plate count''. Generally a lower somatic cell count indicates better animal health, while the bacteria plate count indicates improved equipment sanitation. Somatic cells originate only from inside the animal's udder, while the bacteria are usually from external contaminations, such as insufficient cleaning of the milk transport equipment or insufficient external cleansing of the cow's udder and teats prior to milking. Milking equipment can also be accidentally knocked or kicked off an animal onto the floor, and contaminants on the barn floor can be sucked into the milk line by the system vacuum. A filter sock or filter disk in the pipeline prevents large particulate contaminants from entering the milk bulk tank, but cannot remove bacterial contamination once it has occurred. For example, as defined by the State of Indiana administrative code, grade A milk shall meet the following standards:
*The bacterial estimate classification shall be "acceptable". *The bacteria count using the standard plate count, direct microscopic count, or plate loop count methods shall be not more than one million (1,000,000) bacteria per milliliter. *The somatic cell count shall be not more than one million (1,000,000) cells per milliliter. *The milk shall not contain drug residues. Milk not meeting these standards shall be designated as undergrade. Undergrade milk may not be sold for human consumption or processing into products for human consumption.
As established, these measurements are taken daily from the milk
bulk tank {{no footnotes, date=June 2013 In dairy farming a bulk milk cooling tank is a large storage tank for cooling and holding milk at a cold temperature until it can be picked up by a milk hauler. The bulk milk cooling tank is an important piece of dai ...
and not from individual cows. This is because testing of individual animals at each milking would be expensive, but it also means that milk from a sick cow is diluted and averaged down by the healthy animals. Recently technological advances have allowed the dairy producer to test animals individually for SCC at every milking. The huge bulk tanks at large farms are accommodating of more sick animals in the herd, without the sick animals affecting the overall milk quality rating. However many different state and governmental agencies (including FDA) inspect each load of milk delivered to the processing facility as well as the processing facilities themselves to ensure that all milk processed through those facilities is safe for all consumers. As discussed in the paper ''Guidelines for Using the DHI Somatic Cell Count Program'':
*The results of many studies suggest that cows with SCC of less than 200,000 are not likely to be infected with major mastitis pathogens, but cows with SCC above 300,000 are probably infected (Smith, 1996). *Herds with bulk tank SCC above 200,000 will have varying degrees of subclinical
mastitis Mastitis is inflammation of the breast or udder, usually associated with breastfeeding. Symptoms typically include local pain and redness. There is often an associated fever and general soreness. Onset is typically fairly rapid and usually occurs ...
present. Data from the National Mastitis Council (1987) show that 6% of the dderquarters in a herd could be expected to be infected in a herd with a bulk tank SCC of 200,000. *At 500,000 SCC, 16% of the quarters may be infected with a 6% reduction in milk production compared to a SCC of 200,000.
In Canada, European Union, Australia, New Zealand, Switzerland, and some US states (e.g., Washington) the somatic cell count shall be not more than 400,000 cells per milliliter. The somatic cell count limit is 750,000 in the majority of the USA and 1,000,000 in Brazil.Determining U.S. Milk Quality Using Bulk-tank Somatic Cell Counts
- Centers for Epidemiology and Animal Health September 2012 Bacteria in milk can come from sources other than the animal. Over time the
milking pipeline A milking pipeline or milk pipeline is a component of a dairy farm animal-milking operation which is used to transfer milk from the animals to a cooling and storage bulk tank. Setup In small dairy farms with less than 100 cows, goats or sheep, t ...
and equipment can become coated with residues such as ''milkstone'' which are not removed by standard detergents and require periodic flushing of equipment with high strength corrosives. Automatic washing equipment for the
bulk tank {{no footnotes, date=June 2013 In dairy farming a bulk milk cooling tank is a large storage tank for cooling and holding milk at a cold temperature until it can be picked up by a milk hauler. The bulk milk cooling tank is an important piece of dai ...
may not effectively clean all interior surfaces, and does not clean the exterior of the bulk tank at all. Milk processors and co-ops purchasing milk routinely award farmers for having the lowest possible SCC counts via "quality bonuses" added to each milk payment to the dairyman.


See also

*
Mastitis in dairy cattle Bovine mastitis is the persistent, inflammatory reaction of the udder tissue due to physical trauma or microorganisms infections. Mastitis, a potentially fatal mammary gland infection, is the most common disease in dairy cattle in the United Stat ...
*
Breed method Breed method is a laboratory technique used for counting microorganisms in milk. It was introduced in 1910 by American biologists Samuel Cate Prescott and Robert Stanley Breed. Purpose It is a method for somatic cell count, to know the number ...


References

{{DEFAULTSORT:Somatic Cell Count Dairy farming Cattle