''Solibacillus'' is a genus of

Gram positive In bacteriology, gram-positive bacteria are bacteria that give a positive result in the Gram stain test, which is traditionally used to quickly classify bacteria into two broad categories according to their type of cell wall. Gram-positive bact ...

, rod shaped, spore-forming bacteria. The first member of ''Solibacillus'' was first isolated in 1999, and was originally called ''Bacillus silvestris''. However, further studies on ''B. silvestris'' found that the organism belonged in a separate genus. ''Solibacillus silvestris'' was proposed, referring to its original designation as a member of ''

Bacillus ''Bacillus'' (Latin "stick") is a genus of Gram-positive, rod-shaped bacteria, a member of the phylum '' Bacillota'', with 266 named species. The term is also used to describe the shape (rod) of other so-shaped bacteria; and the plural ''Bacill ...

'', and the fact that the species was first isolated from soil. ''Bacillus isronensis'' was later reclassified as ''Solibacillus isronensis''. ''S. isronensis'' was isolated from cryotubes that were used to collect air samples from high altitudes, and ''S. kalamii'' was found in an air filter from the

International Space Station The International Space Station (ISS) is the largest modular space station currently in low Earth orbit. It is a multinational collaborative project involving five participating space agencies: NASA (United States), Roscosmos (Russia), JAXA ( ...

.Sielaff AC, Kumar RM, Pal D, Mayilraj S, Venkateswaran K. Solibacillus kalamii sp. nov., isolated from a high-efficiency particulate arrestance filter system used in the International Space Station. International Journal of Systematic and Evolutionary Microbiology. 2017 May 5;67(4):896-901. In 2020, the family ''Planococcaceae'' was merged with the family ''

Caryophanaceae The ''Caryophanaceae'' is a family of Gram-positive bacteria. In 2020, the now defunct family '' Planococcaceae'' was merged into ''Caryophanaceae'' to rectify a nomenclature anomaly. The type genus of this family is ''Caryophanon.'' The family ...

'' due to nomenclature rules under the

International Code of Nomenclature of Prokaryotes The International Code of Nomenclature of Prokaryotes (ICNP) formerly the International Code of Nomenclature of Bacteria (ICNB) or Bacteriological Code (BC) governs the scientific names for Bacteria and Archaea.P. H. A. Sneath, 2003. A short hist ...

. In addition, the family ''

'' underwent several taxonomic changes to account for the large number of phylogenetically unrelated species that were misclassified. Using comparative genomic analyses, the study identified several unique molecular markers (specifically conserved signature indels) could serve as a reliable means to distinguish genera such as ''Solibacillus'' within the family.

Molecular Signatures

conserved signature indels Conserved signature inserts and deletions (CSIs) in protein sequences provide an important category of molecular markers for understanding phylogenetic relationships. CSIs, brought about by rare genetic changes, provide useful phylogenetic markers ...

(CSIs) were identified through genomic analyses for this genus in the following proteins: flagellar hook–basal body protein,

aminodeoxychorismate lyase 4-amino-4-deoxychorismate lyase () is an enzyme that participates in folate biosynthesis by catalyzing the production of PABA by the following reaction :4-amino-4-deoxychorismate \rightleftharpoons 4-aminobenzoate + pyruvate This enzyme belongs ...

, VOC family protein,

DNA topoisomerase IV Topoisomerase IV is one of two Type II topoisomerases in bacteria, the other being DNA gyrase. Like gyrase, topoisomerase IV is able to pass one double-strand of DNA through another double-strand of DNA, thereby changing the linking number of DNA b ...

subunit A, DegV family protein, helicase–exonuclease AddAB subunit AddB, multidrug resistance efflux transporter family protein, heme-dependent

peroxidase Peroxidases or peroxide reductases ( EC numberbr>1.11.1.x are a large group of enzymes which play a role in various biological processes. They are named after the fact that they commonly break up peroxides. Functionality Peroxidases typically ca ...

, methionine ABC transporter ATP-binding protein, tRNA 4-thiouridine(8) synthase ThiI, and AAA family ATPase. These CSIs provide a reliable molecular method for distinguishing ''Solibacillus'' species from other genera within the family ''Caryophanaceae'' and all other bacteria.

References

{{Taxonbar, from=Q26286028 Gram-positive bacteria Bacteria genera