The Simulated Fluorescence Process (SFP) is a computing
algorithm
In mathematics and computer science, an algorithm () is a finite sequence of rigorous instructions, typically used to solve a class of specific problems or to perform a computation. Algorithms are used as specifications for performing ...
used for
scientific visualization
Scientific visualization ( also spelled scientific visualisation) is an interdisciplinary branch of science concerned with the visualization of scientific phenomena. Michael Friendly (2008)"Milestones in the history of thematic cartography, s ...
of
3D data from, for example,
fluorescence microscope
A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or absorption, to study the properties of organic or inorganic substances. "Fluorescence microsco ...
s. By
modeling
A model is an informative representation of an object, person or system. The term originally denoted the plans of a building in late 16th-century English, and derived via French and Italian ultimately from Latin ''modulus'', a measure.
Models c ...
a physical light/matter interaction process, an image can be computed which shows the data as it would have appeared in reality when viewed under these conditions.
Principle
The algorithm considers a virtual light source producing excitation light that illuminates the object. This casts shadows either on parts of the object itself or on other objects below it. The interaction between the excitation light and the object provokes the emission light, which also interacts with the object before it finally reaches the eye of the viewer.
See also
*
Computer graphics lighting
Computer graphics lighting is the collection of techniques used to simulate light in computer graphics scenes. While lighting techniques offer flexibility in the level of detail and functionality available, they also operate at different levels of ...
*
Rendering (computer graphics)
Rendering or image synthesis is the process of generating a photorealistic or non-photorealistic image from a 2D or 3D model by means of a computer program. The resulting image is referred to as the render. Multiple models can be defined ...
References
H. T. M. van der Voort, G. J. Brakenhoff and M. W. Baarslag.
Three-dimensional visualization methods for confocal microscopy, Journal of Microscopy, Vol. 153, Pt 2, February 1989, pp. 123–132.
Noordmans, Herke Jan, Hans TM van der Voort, and Arnold WM Smeulders.
Spectral volume rendering" IEEE transactions on visualization and computer graphics 6.3 (2000): 196–207.
External links
Freeware SFP renderer
Computational science
Computer graphics algorithms
Visualization (graphics)
Microscopes
Microscopy
Fluorescence
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