The selector technique is a method to amplify and

multiplex Multiplex may refer to: * Multiplex (automobile), a former American car make * Multiplex (comics), a DC comic book supervillain * Multiplex (company), a global contracting and development company * Multiplex (assay), a biological assay which measur ...

genomic DNA.

Process

Genomic DNA is digested with

restriction enzymes A restriction enzyme, restriction endonuclease, REase, ENase or'' restrictase '' is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. Restriction enzymes are one class o ...

, circularized by hybridisation to selectors and subsequently attached to a

vector Vector most often refers to: *Euclidean vector, a quantity with a magnitude and a direction *Vector (epidemiology), an agent that carries and transmits an infectious pathogen into another living organism Vector may also refer to: Mathematic ...

sequence by

ligation Ligation may refer to: * Ligation (molecular biology), the covalent linking of two ends of DNA or RNA molecules * In medicine, the making of a ligature (tie) * Chemical ligation, the production of peptides from amino acids * Tubal ligation, a meth ...

. The procedure results in circular DNA

molecules A molecule is a group of two or more atoms held together by attractive forces known as chemical bonds; depending on context, the term may or may not include ions which satisfy this criterion. In quantum physics, organic chemistry, and bioche ...

with an included general

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pair motif that can be used for amplification by PCR or

RCA The RCA Corporation was a major American electronics company, which was founded as the Radio Corporation of America in 1919. It was initially a patent trust owned by General Electric (GE), Westinghouse, AT&T Corporation and United Fruit Comp ...

Selector construct

A selector consists of two oligonucleotides, one Vector oligonucleotide and one Selector probe. Together they form one Selector with target specific ends on each side of a general primer motif.

Selection mechanisms

# A selector probe hybridizes with both ends of the selected target. # A selector probe hybridizes with one end to the 3’ end of the target and the other end to an internal sequence of the target. The protruding 5' end is cleaved off using

Taq polymerase ''Taq'' polymerase is a thermostable DNA polymerase I named after the thermophilic eubacterial microorganism ''Thermus aquaticus,'' from which it was originally isolated by Chien et al. in 1976. Its name is often abbreviated to ''Taq'' or ''Ta ...

Publications

Demonstration of the selector methodThe PieceMaker software for designing selector experiments
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