Rhodococcus

''Rhodococcus'' is a genus of aerobic, nonsporulating, nonmotile Gram-positive bacteria closely related to ''Mycobacterium'' and ''Corynebacterium''. While a few species are pathogenic, most are benign, and have been found to thrive in a broad range of environments, including soil, water, and eukaryotic cells. Some species have large genomes, including the 9.7 megabasepair genome (67% G/C) of ''Rhodococcus'' sp. RHA1.

Strains of ''Rhodococcus'' are important owing to their ability to catabolize a wide range of compounds and produce bioactive steroids, acrylamide, and acrylic acid, and their involvement in fossil fuel biodesulfurization. This genetic and catabolic diversity is not only due to the large bacterial chromosome, but also to the presence of three large linear plasmids. ''Rhodococcus'' is also an experimentally advantageous system owing to a relatively fast growth rate and simple developmental cycle, but is not well characterized.

Another important application of ''Rhodococcus'' comes from bioconversion, using biological systems to convert cheap starting material into more valuable compounds, such as its ability to metabolize harmful environmental pollutants, including toluene, naphthalene, herbicides, and PCBs. ''Rhodococcus'' species typically metabolize aromatic substrates by first oxygenating the aromatic ring to form a diol (two alcohol groups). Then, the ring is cleaved with intra/extradiol mechanisms, opening the ring and exposing the substrate to further metabolism. Since the chemistry is very stereospecific, the diols are created with predictable chirality. While controlling the chirality of chemical reaction presents a significant challenge for synthetic chemists, biological processes can be used instead to faithfully produce chiral molecules in cases where direct chemical synthesis is not feasible or efficient. An example of this is the use of ''Rhodococcus'' to produce chiral indandiol derivatives which serve as synthetic intermediates for indinavir, a protease inhibitor used in the treatment of HIV/AIDS.

Biodegradation of organic pollutants

''Rhodococcus'' has been greatly researched as a potential agent for the bioremediation of pollutants as it is commonly found in the natural environment, and they possess certain characteristics that allow them to thrive under a variety of conditions, and they have the capability to metabolize many hydrocarbons.

Rhodococci possess many properties that makes them suitable for bioremediation under a range of environments. Their ability to undergo microaerophilic respiration allows them to survive in environments containing low oxygen concentrations, and their ability to undergo aerobic respiration also allows them to survive in oxygenated environments. They also undergo nitrogen fixation, which allows them to generate their own nutrients in environments with low nutrients.

Rhodococci also contain characteristics that enhances their ability to degrade organic pollutants. Their hydrophobic surface allows for adhesion to hydrocarbons, which enhances its ability to degrade these pollutants. They have a wide variety of catabolic pathways and many unique enzyme functions. This gives them the ability to degrade many recalcitrant, toxic hydrocarbons. For example, Rhodococci expresses dioxygenases, which can be used to degrade benzotrifluoride, a recalcitrant pollutant. ''Rhodococcus'' sp. strain Q1, a strain naturally found in soil and paper mill sludge, contains the ability to degrade quinoline, various pyridine derivatives, catechol, benzoate, and protocatechuic acid. Rhodococci are also capable of accumulating heavy metal ions, such as radioactive caesium, allowing for easier removal from the environment. Other pollutants, such as azo dyes, pesticides and polychlorinated biphenyls can also be degraded by Rhodococci.

Pathogenic ''Rhodococcus''

The genus ''Rhodococcus'' has two pathogenic species: '' R. fascians'' and '' R. equi''. The former, a plant pathogen, causes leafy gall disease in both angiosperm and gymnosperm plants. ''R. equi'' is the causative agent of foal pneumonia (rattles) and mainly infects foals up to three months in age. However, it has a wide host range, sporadically infecting pigs, cattle, and immunocompromised humans, in particular AIDS patients and those undergoing immunosuppressive therapy. Both pathogens rely on a conjugative virulence plasmid to cause disease. In case of ''R. fascians'', this is a linear plasmid, whereas ''R. equi'' harbors a circular plasmid. Both pathogens are economically significant. ''R. fascians'' is a major pathogen of tobacco plants. ''R. equi'', one of the most important foal pathogens, is endemic on many stud farms around the world.

In molecular biology

''Rhodococcus'' has also been identified as a contaminant of DNA extraction kit reagents and ultrapure water systems, which may lead to its erroneous appearance in microbiota or metagenomic datasets.

Species

''Rhodococcus'' comprises the following species:

* '' R. aerolatus'' Hwang et al. 2015
* '' R. aetherivorans'' Goodfellow et al. 2004
* '' R. agglutinans'' Guo et al. 2015

* '' R. antrifimi'' Ko et al. 2015
* '' R. artemisiae'' Zhao et al. 2012

* "'' R. australis''" Hiddema et al. 1985

* "'' R. boritolerans''" Lin et al. 2012

* '' R. canchipurensis'' Nimaichand et al. 2013
* '' R. cavernicola'' Lee et al. 2020
* '' R. cerastii'' Kämpfer et al. 2013
* '' R. cercidiphylli'' Li et al. 2012

* '' R. chubuensis'' Tsukamura 1983
* '' R. coprophilus'' Rowbotham and Cross 1979 (Approved Lists 1980)

* '' R. corynebacterioides'' (Serrano et al. 1972) Yassin and Schaal 2005
* "'' R. daqingensis''" Wang et al. 2019
* '' R. defluvii'' Kämpfer et al. 2014

* '' R. electrodiphilus'' Ramaprasad et al. 2018

* '' R. equi'' (Magnusson 1923) Goodfellow and Alderson 1977 (Approved Lists 1980)
* '' R. erythropolis'' (Gray and Thornton 1928) Goodfellow and Alderson 1979 (Approved Lists 1980)
* '' R. fascians'' (Tilford 1936) Goodfellow 1984
* '' R. gannanensis'' Ma et al. 2017
* '' R. globerulus'' Goodfellow et al. 1985
* '' R. gordoniae'' Jones et al. 2004

* '' R. humicola'' Nguyen and Kim 2016

* '' R. jostii'' Takeuchi et al. 2002
* '' R. koreensis'' Yoon et al. 2000
* "'' R. kronopolitis''" Liu et al. 2014
* '' R. kroppenstedtii'' Mayilraj et al. 2006

* '' R. kyotonensis'' Li et al. 2007
* '' R. lactis'' Singh et al. 2015

* '' R. maanshanensis'' Zhang et al. 2002
* '' R. marinonascens'' Helmke and Weyland 1984

* '' R. nanhaiensis'' Li et al. 2012
* '' R. obuensis'' Tsukamura 1983
* '' R. olei'' Chaudhary and Kim 2018
* '' R. opacus'' Klatte et al. 1995
* '' R. oryzae'' Li et al. 2020
* '' R. pedocola'' Nguyen and Kim 2016

* '' R. phenolicus'' Rehfuss and Urban 2006
* "'' R. psychrotolerans''" Silva et al. 2018
* '' R. pyridinivorans'' Yoon et al. 2000

* '' R. rhodnii'' Goodfellow and Alderson 1979 (Approved Lists 1980)
* '' R. rhodochrous'' (Zopf 1891) Tsukamura 1974 (Approved Lists 1980)

* '' R. ruber'' (Kruse 1896) Goodfellow and Alderson 1977 (Approved Lists 1980)

* '' R. soli'' Li et al. 2015
* '' R. sovatensis'' Táncsics et al. 2017
* '' R. spelaei'' Lee and Kim 2021
* '' R. spongiicola'' Zhang et al. 2021

* '' R. subtropicus'' Lee et al. 2019

* '' R. triatomae'' Yassin 2005
* '' R. trifolii'' Kämpfer et al. 2013
* '' R. tukisamuensis'' Matsuyama et al. 2003
* '' R. wratislaviensis'' (Goodfellow et al. 1995) Goodfellow et al. 2002
* '' R. xishaensis'' Zhang et al. 2021
* '' R. yunnanensis'' Zhang et al. 2005
* '' R. zopfii'' Stoecker et al. 1994

Notes

References

{{Taxonbar, from=Q288311

Mycobacteriales
Bacteria genera