Rappaport Vassiliadis soya peptone broth
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Rappaport-Vassiliadis soya peptone broth (RVS broth) is used as an enrichment growth medium for the isolation of '' Salmonella'' species. It is not recommended for the enrichment of ''Salmonella'' Typhi or Paratyphi, which is inhibited due to the malachite green in RVS broth. It is an alternative to selenite broth. It is not associated with potential
teratogenicity Teratology is the study of abnormalities of physiological development in organisms during their life span. It is a sub-discipline in medical genetics which focuses on the classification of congenital abnormalities in dysmorphology. The related ...
problems seen with the use of selenite broth. It enriches salmonellae because they are better able to survive the high osmotic pressure in the medium and because they can multiply at relatively lower pH and higher temperatures compared with other gut bacteria. RVS broth has a pH around 5.2.


Components

A liter of RVS broth contains: *4.5g Soya peptone *7.2g Sodium chloride *1.26g Potassium dihydrogen phosphate *0.18g
Dipotassium phosphate Dipotassium phosphate (K2HPO4) (also dipotassium hydrogen orthophosphate; potassium phosphate dibasic) is the inorganic compound with the formula K2HPO4.(H2O)x (x = 0, 3, 6). Together with monopotassium phosphate (KH2PO4.(H2O)x), it is often used ...
*13.58g
Magnesium chloride Magnesium chloride is the family of inorganic compounds with the formula , where x can range from 0 to 12. These salts are colorless or white solids that are highly soluble in water. These compounds and their solutions, both of which occur in natu ...
(anhydrous) * 0.036g
Malachite green Malachite green is an organic compound that is used as a dyestuff and controversially as an antimicrobial in aquaculture. Malachite green is traditionally used as a dye for materials such as silk, leather, and paper. Despite its name the dye is ...


References

{{Reflist Microbiological media Bacteriology