The CyaR RNA (formerly known as RyeE RNA)
non-coding RNA
A non-coding RNA (ncRNA) is a functional RNA molecule that is not translated into a protein. The DNA sequence from which a functional non-coding RNA is transcribed is often called an RNA gene. Abundant and functionally important types of non-c ...
was identified in a large scale screen of ''
Escherichia coli
''Escherichia coli'' (),Wells, J. C. (2000) Longman Pronunciation Dictionary. Harlow ngland Pearson Education Ltd. also known as ''E. coli'' (), is a Gram-negative, facultative anaerobic, rod-shaped, coliform bacterium of the genus ''Escher ...
'' and was called candidate 14.
The exact 5′ and 3′ ends of this RNA are uncertain. This gene lies between yegQ and orgK in ''E. coli''. This small RNA was shown to be bound by the
Hfq protein
The Hfq protein (also known as HF-I protein) encoded by the ''hfq'' gene was discovered in 1968 as an ''Escherichia coli'' host factor that was essential for replication of the bacteriophage Qβ. It is now clear that Hfq is an abundant bacterial RN ...
. This RNA has been renamed as CyaR for (cyclic AMP-activated
RNA). It has been shown that the CyaR RNA acts as a repressor of the
porin OmpX.
It has also been shown that ''cyaR'' expression is tightly controlled by the cyclic AMP receptor protein,
CRP.
A
comparative genomics
Comparative genomics is a field of biological research in which the genomic features of different organisms are compared. The genomic features may include the DNA sequence, genes, gene order, regulatory sequences, and other genomic structural lan ...
approach was able to detect and verify the additional targets ''ptsI'', ''sdhA'' and ''yobF''.
See also
*
RydB RNA
*
RyhB RNA
RyhB RNA is a 90 nucleotide RNA that down-regulates a set of iron-storage and iron-using proteins when iron is limiting; it is itself negatively regulated by the ferric uptake repressor protein, Fur ( Ferric uptake regulator).
Discovery
The gene ...
*
RyeB RNA
References
External links
*
Non-coding RNA
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