A Roux culture bottle, or simply Roux bottle, is a type of

laboratory glassware Laboratory glassware refers to a variety of equipment used in scientific work, and traditionally made of glass. Glass can be blown, bent, cut, molded, and formed into many sizes and shapes, and is therefore common in chemistry, biology, and anal ...

used in

biology Biology is the scientific study of life. It is a natural science with a broad scope but has several unifying themes that tie it together as a single, coherent field. For instance, all organisms are made up of cells that process hereditary i ...

and related sciences to grow

microorganisms A microorganism, or microbe,, ''mikros'', "small") and ''organism'' from the el, ὀργανισμός, ''organismós'', "organism"). It is usually written as a single word but is sometimes hyphenated (''micro-organism''), especially in olde ...

or tissue cells. It consists of a bottle of transparent glass or plastic with two closely spaced flat, rectangular, parallel faces and a short neck; of such a design that the bottle can be laid down sideways, on one of those two faces, even when unstoppered and partially filled with a

culture medium A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation or small plants like the moss ''Physcomitrella patens''. Different ...

. This goal is achieved by having the neck narrowed, offset, partially blocked, or canted (tilted). The item is also generically called cell culture bottle or tissue culture (TC) bottle, and flask may be used instead of "bottle". Pile of culture flask (385575937)

A Roux bottle provides a large surface for the cells or microorganisms to grow, whether on the top of, floating in, or at the bottom of the medium. The flat upper face then allows inspection of the culture and even illumination for

photosynthetic Photosynthesis is a process used by plants and other organisms to convert light energy into chemical energy that, through cellular respiration, can later be released to fuel the organism's activities. Some of this chemical energy is stored in c ...

organisms. Organisms growing at the bottom of the medium can also be inspected from below, with an

inverted microscope An inverted microscope is a microscope with its light source and condenser on the top, above the stage pointing down, while the objectives and turret are below the stage pointing up. It was invented in 1850 by J. Lawrence Smith, a faculty membe ...

. The bottle is typically closed with a plug or cap that prevents contamination of the culture by spores from the outside, while still allowing exchange of gases generated or consumed by the organisms inside. The bottle's invention has been attributed to French physician and

Pasteur Louis Pasteur (, ; 27 December 1822 – 28 September 1895) was a French chemist and microbiologist renowned for his discoveries of the principles of vaccination, microbial fermentation and pasteurization, the latter of which was named after ...

's collaborator

Pierre Roux Pierre is a masculine given name. It is a French language, French form of the name Peter (given name), Peter. Pierre originally meant "rock" or "stone" in French (derived from the Greek word πέτρος (''petros'') meaning "stone, rock", via ...

(1853-1933).

Variations

The "DeLong" type has a horizontal neck with a low barrier at the base. There is a cylindrical version that is meant to be slowly tuned on rollers to keep the contents well mixed.

References

Erhard F. Kaleta and Catherine P. A. Rülke (2009): "The beginning and spread of Fowl Plague (H7 High Pathogenicity Avian Influenza) across Europe and Asia (1878-1955)". In David E. Swayne (ed),
Avian Influenza
'. pages 145-189. Erhard F. Kaleta (2006):
A Brief History, Modes of Spread and Impact of Fowl Plague Viruses
. ''Asia-Pacific Biotech News'', volume 10, issue 14, pages 717-725. Christopher G. Morris, Christopher W. Morris ():
Academic Press Dictionary of Science and Technology
'. 2432 pages. Orioner, Malaysia (2020):
Pigeons Type Cell Culture Bottle by Sichuan Shubo
. Online catalog item 1481-145. Accessed on 2020-02-21. Sarstedt Co. (2020):
TC Flask T175,Standard
. Online catalog item 83.3912. Accessed on 2020-02-21. Chemglass Co. (2020):
BOTTLES, ROUX, 1200ML
. Online catalog item CLS-1195-1200. Accessed on 2020-02-21. Kisker Biotech GmbH (2020):
Roux flask 38 mm 1200 mL long neck
. Online catalog item 202940. Accessed on 2020-02-21. Sigma-Aldrich (2020):
Pyrex Roux culture flask, off-set neck
Online catalog entry SLW1350/04M. Accessed on 2020-02-21. Carl Roth GmbH (2015):
Cell culture bottles Filter screw cap
. Online catalog item CE48.1. Accessed on 2020-02-21. Carl Roth GmbH (2015):
Cell culture bottles CELLMASTER Plastic roller bottles Filter screw cap
. Online catalog item CE59.1. Accessed on 2020-02-21. Bellco Glass (2020):
DeLong Roux Culture Bottle 1200mL Schott Blank
. Online catalog item, SKU 5630-12000. Accessed on 2020-02-21. Marjory Stephenson and Margaret Dampier Whetham (1922): "Studies in the Fat Metabolism of the Timothy Grass Bacillus". ''Proceedings of the Royal Society of London, Series B - Biology'', volume 93, pages 262-280. Sophie A. Leliévre (2015):
Cell Culture Basics 11: Adding medium to a culture flask with attached cells
. Lab technique training video produced by Purdue University, uploaded to YouTube.com on 2015-09-23. Accessed on 2020-02-21. Abnova (2011):
Cell Culture (Attached Cell)
. Technical instruction video, uploaded to YouTube on 2011-01-13. Accessed on 2020-02-21. Blaine Carter and Michael Huesemann (2008):
Hydrogen production by the cyanobacterium Plectonema boryanum: Effects of initial nitrate concentration, light intensity, and inhibitionof photosystem II by DCMU
. U.S. Department of Energy, ''Journal of Undergraduate Research'', OSTI article 1052083, volume 8, pages 23-32. John W. Eyre (1913): ''Bacteriological Techniques: A Laboratory Guide''. Reprinted as Volume 39 of ''Historical Science'' series, Books on Demand, 2010; 556 pages. {{isbn, 9783867412568 Laboratory glassware

Variations

See also

References