Random chimeragenesis on transient templates (RACHITT) is a method to perform molecular mutagenesis at a high recombination rate. For example, RACHITT can be used to generate increased rate and extent of biodesulfurization of diesel by modification of dibenzothiophene mono-oxygenase.

DNA shuffling DNA shuffling, also known as molecular breeding, is an in vitro random recombination method to generate mutant genes for directed evolution and to enable a rapid increase in DNA library size. Three procedures for accomplishing DNA shuffling are mo ...

is a similar but less powerful method used in directed evolution experiments.

References

Chimerism {{molecular-biology-stub